Detailed information NTG0280115

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C4K03_RS04610
• Genome accession: NZ_CP027754
• Coordinates: 1001132..1001998 (-)
• Length: 288 a.a.
• NCBI ID: WP_124376300.1
• Uniprot ID: A0A3G7U3J5
• Organism: Pseudomonas synxantha strain 30B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 996132..1006998

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4K03_RS04575 (C4K03_0897)	-	996829..998127 (-)	1299	WP_046070857.1	NAD(P)/FAD-dependent oxidoreductase	-
C4K03_RS04580 (C4K03_0898)	-	998188..998361 (-)	174	WP_046070858.1	DUF3094 family protein	-
C4K03_RS04585 (C4K03_0899)	-	998450..999079 (-)	630	WP_043049309.1	DUF1780 domain-containing protein	-
C4K03_RS04590 (C4K03_0900)	-	999349..1000038 (+)	690	WP_124376298.1	energy-coupling factor ABC transporter permease	-
C4K03_RS04595 (C4K03_0901)	-	1000103..1000318 (+)	216	WP_003171680.1	hypothetical protein	-
C4K03_RS04600 (C4K03_0902)	yacG	1000315..1000515 (-)	201	WP_043049311.1	DNA gyrase inhibitor YacG	-
C4K03_RS04605 (C4K03_0903)	coaE	1000512..1001135 (-)	624	WP_124376299.1	dephospho-CoA kinase	-
C4K03_RS04610 (C4K03_0904)	pilD	1001132..1001998 (-)	867	WP_124376300.1	A24 family peptidase	Machinery gene
C4K03_RS04615 (C4K03_0905)	pilC	1001995..1003203 (-)	1209	WP_124376301.1	type II secretion system F family protein	Machinery gene
C4K03_RS04620 (C4K03_0906)	-	1003405..1003818 (+)	414	WP_057013848.1	pilin	-
C4K03_RS04630 (C4K03_0908)	-	1004106..1004411 (+)	306	WP_124376302.1	DUF6388 family protein	-
C4K03_RS04635 (C4K03_0909)	nadC	1004531..1005379 (-)	849	WP_124376303.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31817.25 Da
• Isoelectric Point: 8.1704

>NTDB_id=280115 C4K03_RS04610 WP_124376300.1 1001132..1001998(-) (pilD) [Pseudomonas synxantha strain 30B]
MILLLTERPWFFVAMALVLGLIVGSFLNVLVWRLPKMLEREWRAQAHEILGLPAVPAGPAYNLMRPNSCCTRCEQPIRPW
ENIPLLSYLLLRGRCAHCREPISLRYPLTELACALISATVAWHFGFGWHAGAVLLLSWGLLGLSLIDLDHQLLPDALVLP
LLWLGLILNSADLLAALPDALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMASLLGLI
AGLILLRLRKAPVSAPVPFGPCLAITGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=280115 C4K03_RS04610 WP_124376300.1 1001132..1001998(-) (pilD) [Pseudomonas synxantha strain 30B]
TTGATCCTGCTGCTGACCGAACGGCCCTGGTTTTTTGTTGCCATGGCACTGGTGCTGGGCCTGATCGTCGGCAGTTTCCT
CAATGTGCTGGTGTGGCGACTGCCAAAAATGCTCGAGCGAGAATGGCGTGCCCAGGCCCATGAGATCCTCGGCTTGCCTG
CCGTACCCGCCGGGCCGGCCTATAACCTGATGCGCCCGAACTCCTGCTGCACCCGTTGCGAACAGCCGATTCGGCCCTGG
GAAAATATTCCACTGCTCAGCTACCTGTTGCTCAGGGGGCGTTGTGCCCATTGCCGTGAGCCCATCAGCCTTCGTTACCC
CTTAACCGAGCTGGCCTGCGCGTTGATCTCGGCCACCGTTGCCTGGCACTTCGGTTTTGGCTGGCACGCCGGCGCGGTGT
TGTTGCTGAGCTGGGGCTTACTGGGCCTGAGCCTGATTGATCTCGACCATCAATTGTTACCGGATGCGCTGGTGCTGCCG
CTGCTATGGCTGGGACTCATCCTCAACAGCGCCGACCTGCTGGCGGCCTTGCCCGATGCGCTGTGGGGCGCGGTCATTGG
CTACATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTCACCGGTAAAGACGGCATGGGCCATGGCGACTTCAAGT
TATTGGCATTGTTGGGAGCCTGGGGCGGCTGGCAGATACTGCCGATGACATTGCTGATGGCCTCACTGCTGGGCTTGATT
GCCGGGCTGATTCTATTGCGTTTGCGCAAGGCCCCGGTGTCAGCACCAGTGCCATTTGGTCCCTGCCTGGCAATTACCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTACTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (135-243)

3D structure

• AlphaFold DB: A0A3G7U3J5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.237	96.528	0.514
pilD	Vibrio campbellii strain DS40M4	50.538	96.875	0.49
pilD	Acinetobacter baumannii D1279779	48.352	94.792	0.458
pilD	Acinetobacter nosocomialis M2	48.352	94.792	0.458
pilD	Neisseria gonorrhoeae MS11	50	90.278	0.451