Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K03_RS02060 Genome accession   NZ_CP027754
Coordinates   467332..468657 (+) Length   441 a.a.
NCBI ID   WP_124380059.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain 30B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462332..473657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K03_RS02040 (C4K03_0406) - 462395..464860 (-) 2466 WP_124375954.1 penicillin-binding protein 1A -
  C4K03_RS02045 (C4K03_6296) pilM 465091..465746 (+) 656 Protein_393 type IV pilus assembly protein PilM -
  C4K03_RS02050 (C4K03_0408) - 465743..466294 (+) 552 WP_124375955.1 PilN domain-containing protein -
  C4K03_RS02055 (C4K03_0409) - 466291..467262 (+) 972 WP_124375956.1 pilus assembly protein PilP -
  C4K03_RS02060 (C4K03_0410) pilQ 467332..468657 (+) 1326 WP_124380059.1 type IV pilus secretin PilQ family protein Machinery gene
  C4K03_RS02065 (C4K03_0411) aroK 468662..469180 (+) 519 WP_003187914.1 shikimate kinase AroK -
  C4K03_RS02070 (C4K03_0412) aroB 469306..470406 (+) 1101 WP_124375957.1 3-dehydroquinate synthase -
  C4K03_RS02075 (C4K03_0413) - 470417..471988 (+) 1572 WP_124375958.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48090.05 Da        Isoelectric Point: 5.0313

>NTDB_id=280114 C4K03_RS02060 WP_124380059.1 467332..468657(+) (pilQ) [Pseudomonas synxantha strain 30B]
MAAAVPNRPDLIHLPPPDRALSVSYTGEKLTLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLVLESRKQMADLAPLRRELLQVNYAKAADLAKLFQSVTGFEGSTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGISKPLADGAEPPQ
KPPSSPFVDMGSLTGTSGLGIAYITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRNVLSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=280114 C4K03_RS02060 WP_124380059.1 467332..468657(+) (pilQ) [Pseudomonas synxantha strain 30B]
ATGGCTGCCGCTGTGCCCAATCGCCCGGACCTGATCCACCTGCCGCCGCCTGACCGTGCCTTGTCGGTCAGCTATACGGG
CGAAAAATTGACCCTCAATTTCCAGGACATCGACCTGCGGGCGGTATTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGCGACGAGGTGCAGGGCTCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGACCAGGCGCTGGACCTGGTG
CTGCAAACCAAGGGCCTGGACAAGCGGGTGAAGGCCGGCGTGTTGCTGGTGGCGCCCGCCGATGAATTGGCCGCTCGCGA
ACTGCTGGTGCTGGAGTCGCGCAAGCAGATGGCCGACCTGGCACCGTTGCGTCGGGAGTTATTGCAGGTCAATTACGCCA
AGGCTGCGGACCTGGCCAAGCTGTTCCAGTCGGTGACCGGTTTTGAGGGCTCCACGGATGAACGCGGCTCGGTGGCGGTG
GATGACCGCACCAACAACATCATCGCCTACCAGACCGGCGAACGCCTCGAAGAGCTGCGGCGCATCGTGGCGCAGTTGGA
TATCCCGGTACGTCAGGTGATGATCGAGGCGCGAATCGTCGAGGCCAATGTCGACTACGACAAAAGCCTTGGCGTGCGCT
GGGGTGGGCGGCTCAGCCGCGGCAACTGGGGCGCCGGAGGTATTTCCAAGCCTCTGGCGGACGGCGCCGAGCCCCCGCAA
AAACCGCCCAGTTCACCCTTCGTCGACATGGGTTCGCTGACCGGCACGTCGGGGCTGGGTATCGCCTACATCACCGACAA
CCTGTTGCTGGATTTAGAACTGACGGCGATGGAGAAAAGCGGCAACGGCGAAATCGTTTCGCAGCCCAAGGTGGTCACGT
CCGATAAAGAGACTGCACGCATACTCAAAGGCACCGAGATTCCCTACCAGGAGTCCACCTCCCAGGGCGCTACCTCGGTA
TCGTTCAAGGAAGCGTCCCTTTCACTGGAGGTCACGCCGCAAATCACTCCCGATGACCATGTGATCATGGCGGTCAAGGT
CACCAAGGACGAGCCCGACTACCTGAACCGGCTCAATGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGC
TGGTTCGGGATGGCGAGACTATTGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTACCGTATCTTGGCCGCCTTTTCCGGCGCAATGTGCTGTCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Secretin and TonB N terminus short domain (STN)

(54-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.615

99.546

0.703
  comQ Acinetobacter baylyi ADP1

43.142

100

0.442
  pilQ Acinetobacter baumannii strain A118

40.645

100

0.429
  pilQ Acinetobacter baumannii D1279779

40.645

100

0.429
  pilQ Legionella pneumophila strain Lp02

44.762

95.238

0.426
  pilQ Vibrio campbellii strain DS40M4

43.418

98.186

0.426
  pilQ Legionella pneumophila strain ERS1305867

44.286

95.238

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.459

97.732

0.415
  pilQ Vibrio cholerae strain A1552

42.459

97.732

0.415

Multiple sequence alignment