Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4J94_RS04495 Genome accession   NZ_CP027727
Coordinates   1002465..1003673 (-) Length   402 a.a.
NCBI ID   WP_124385091.1    Uniprot ID   A0A3G7Y859
Organism   Pseudomonas sp. R5-89-07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 997465..1008673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J94_RS04460 (C4J94_0878) - 998768..998941 (-) 174 WP_124388919.1 DUF3094 family protein -
  C4J94_RS04465 (C4J94_0879) - 998994..999623 (-) 630 WP_016971624.1 DUF1780 domain-containing protein -
  C4J94_RS04470 (C4J94_0880) - 999820..1000509 (+) 690 WP_124385088.1 energy-coupling factor ABC transporter permease -
  C4J94_RS04475 (C4J94_0881) - 1000573..1000788 (+) 216 WP_003171680.1 hypothetical protein -
  C4J94_RS04480 (C4J94_0882) yacG 1000785..1000985 (-) 201 WP_065887991.1 DNA gyrase inhibitor YacG -
  C4J94_RS04485 (C4J94_0883) coaE 1000982..1001605 (-) 624 WP_124385089.1 dephospho-CoA kinase -
  C4J94_RS04490 (C4J94_0884) pilD 1001602..1002468 (-) 867 WP_124385090.1 A24 family peptidase Machinery gene
  C4J94_RS04495 (C4J94_0885) pilC 1002465..1003673 (-) 1209 WP_124385091.1 type II secretion system F family protein Machinery gene
  C4J94_RS04500 (C4J94_0886) - 1003872..1004285 (+) 414 WP_124388920.1 pilin -
  C4J94_RS04510 (C4J94_0888) - 1004586..1004891 (+) 306 WP_124385092.1 DUF6388 family protein -
  C4J94_RS04515 (C4J94_0889) nadC 1005010..1005858 (-) 849 WP_124385093.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4J94_RS04520 (C4J94_0890) - 1006105..1008261 (+) 2157 WP_124385094.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43363.83 Da        Isoelectric Point: 10.3218

>NTDB_id=279473 C4J94_RS04495 WP_124385091.1 1002465..1003673(-) (pilC) [Pseudomonas sp. R5-89-07]
MNDTSTIYTWQGINRKGRRVCGQTAGHDPALIKAQLRQRGICPERVRKKPSGRPSLAAPIQPADIALFTRQLATLLRAGI
PLLQAFDIISEGIENRQLRELVRGLKQHVAAGNSLAGTLRQFPRYFDDLYCNLIAAGEQAGALETLLERVAIHLEKSQRL
KTRIKKAMTYPLAVLVVASLVSAVLLIYVVPQFRHLFAGVGSQLPGFTLGVIALSDFLQRAWWILALGLGAALVGLRHAY
RVSPGFRHALEAGLLKAPLAGKLLNKAAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFRQAIEHMRHDVSTGMPLN
QSMATSGLFPGMAIQMTAIGEESGTLEHMLEKVANHYEADVETLVDNLTSLMEPLIMVVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=279473 C4J94_RS04495 WP_124385091.1 1002465..1003673(-) (pilC) [Pseudomonas sp. R5-89-07]
ATGAACGACACCTCAACGATCTATACCTGGCAAGGCATCAACCGCAAAGGCCGCAGGGTGTGCGGGCAAACCGCCGGCCA
CGACCCTGCGCTGATCAAGGCACAACTGCGCCAGCGAGGGATCTGCCCCGAGCGTGTGCGCAAGAAACCCTCAGGCCGGC
CAAGCCTGGCTGCGCCGATACAGCCTGCGGACATTGCTCTGTTCACCCGCCAGTTGGCGACGCTGCTGAGGGCGGGTATC
CCGCTGTTGCAAGCCTTCGATATCATCAGCGAGGGCATCGAAAACCGGCAACTGCGCGAGCTGGTCAGGGGCCTGAAACA
ACACGTCGCCGCTGGCAATAGCCTGGCCGGCACACTGCGCCAATTTCCACGGTATTTCGATGACCTGTACTGCAATCTGA
TTGCCGCTGGTGAGCAGGCAGGTGCTCTGGAAACGCTGCTTGAGCGAGTGGCCATTCACCTGGAAAAAAGCCAACGGCTC
AAGACCAGAATCAAGAAAGCCATGACCTACCCTCTCGCAGTGCTGGTGGTGGCCAGCCTGGTCAGCGCTGTGCTGTTAAT
ATATGTGGTGCCGCAGTTCCGGCACCTGTTTGCCGGGGTTGGCAGCCAATTGCCCGGTTTTACCCTAGGGGTGATCGCCC
TCTCCGACTTCCTTCAACGGGCCTGGTGGATCCTGGCCCTGGGCCTGGGCGCGGCATTAGTCGGGCTGCGTCATGCCTAC
CGCGTTTCGCCTGGCTTTCGCCACGCGCTGGAGGCAGGTTTGTTGAAAGCCCCCCTGGCAGGCAAACTGTTGAATAAAGC
GGCCGTCGCCCGCTACGCCCGCACGCTTTCAACCACGTTTGCGGCAGGCGTTCCCCTGGTACAGGCACTGGGCTCAGTGG
CCGGTGCCGTCGGTAATGGCCCCTTCAGACAGGCAATCGAACATATGCGCCACGATGTATCCACAGGCATGCCGTTGAAT
CAATCCATGGCCACCAGCGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCCGGCACGCTGGA
GCACATGCTGGAAAAGGTCGCGAACCATTACGAAGCTGACGTAGAGACCCTCGTCGATAACCTCACCAGCCTCATGGAAC
CGCTGATCATGGTCGTACTGGGGAGCATCGTCGGTGCGCTGGTGATCGCCATGTACCTGCCAGTGTTTCAATTGGGTTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G7Y859

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

98.507

0.565
  pilC Acinetobacter baylyi ADP1

51.515

98.507

0.507
  pilC Legionella pneumophila strain ERS1305867

48.615

98.756

0.48
  pilC Acinetobacter baumannii D1279779

48.608

98.259

0.478
  pilG Neisseria gonorrhoeae MS11

40.314

95.025

0.383
  pilG Neisseria meningitidis 44/76-A

39.791

95.025

0.378
  pilC Vibrio cholerae strain A1552

38.095

99.254

0.378
  pilC Thermus thermophilus HB27

37.343

99.254

0.371
  pilC Vibrio campbellii strain DS40M4

37.31

98.01

0.366

Multiple sequence alignment