Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4J95_RS04025 Genome accession   NZ_CP027726
Coordinates   919341..920549 (-) Length   402 a.a.
NCBI ID   WP_124356999.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain R4-35-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 914341..925549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J95_RS03985 (C4J95_0795) - 914351..915649 (-) 1299 WP_124356993.1 NAD(P)/FAD-dependent oxidoreductase -
  C4J95_RS03990 (C4J95_0796) - 915710..915883 (-) 174 WP_124356994.1 DUF3094 family protein -
  C4J95_RS03995 (C4J95_0797) - 915937..916566 (-) 630 WP_124356995.1 DUF1780 domain-containing protein -
  C4J95_RS04000 (C4J95_0798) - 916696..917385 (+) 690 WP_124356996.1 energy-coupling factor ABC transporter permease -
  C4J95_RS04005 (C4J95_0799) - 917449..917664 (+) 216 WP_003171680.1 hypothetical protein -
  C4J95_RS04010 (C4J95_0800) yacG 917661..917861 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  C4J95_RS04015 (C4J95_0801) coaE 917858..918481 (-) 624 WP_124356997.1 dephospho-CoA kinase -
  C4J95_RS04020 (C4J95_0802) pilD 918478..919344 (-) 867 WP_124356998.1 prepilin peptidase Machinery gene
  C4J95_RS04025 (C4J95_0803) pilC 919341..920549 (-) 1209 WP_124356999.1 type II secretion system F family protein Machinery gene
  C4J95_RS04030 (C4J95_0804) - 920748..921161 (+) 414 WP_124360463.1 pilin -
  C4J95_RS04040 (C4J95_0806) - 921490..921795 (+) 306 WP_124357000.1 DUF6388 family protein -
  C4J95_RS04045 (C4J95_0807) nadC 921916..922764 (-) 849 WP_124357001.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4J95_RS04050 (C4J95_0809) - 923005..925167 (+) 2163 WP_124357002.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43210.56 Da        Isoelectric Point: 9.7366

>NTDB_id=279450 C4J95_RS04025 WP_124356999.1 919341..920549(-) (pilC) [Pseudomonas orientalis strain R4-35-08]
MNDSSTIYAWQGINRKGRRVCGKIAGHDLALVKAQLRAQGICPERVRRQSPGLPSLAAPIRPADIALFTRQLATLLKAGI
ALLQAFDIISEGVENHPLRELVKGLKQQVASGNSLASALRQHPRYFDDLYCHLVAAGEQAGALETLLERVAVHLEKSQQL
KTRIKKAMIYPLAVLVVAGLVSAVLLMQVVPQFQNLFAGVDTPLPGFTLGVIALSDFLRQAWWLLALGLGAAIGGLRHAY
RVSAQFRYRLEAGLLKAPLAGKLSSKAAVARYARTLSTTFAAGVPLVQALDSVAGAVGNGPFRQAIEHMRHDVSTGMQLN
QSMATSGLFPGMAIQMTAIGEESGTLDYMLEKVANHYEADVETLVDNLTSLMEPLIMLVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=279450 C4J95_RS04025 WP_124356999.1 919341..920549(-) (pilC) [Pseudomonas orientalis strain R4-35-08]
ATGAACGACAGCTCGACGATCTACGCCTGGCAAGGCATCAACCGCAAAGGTCGCAGGGTGTGCGGGAAAATCGCAGGGCA
TGACCTTGCGTTGGTCAAGGCACAACTGCGCGCGCAAGGGATCTGCCCCGAGCGTGTGCGCAGGCAATCGCCAGGACTGC
CAAGCCTGGCTGCGCCGATACGGCCAGCGGACATCGCCCTGTTCACCCGCCAGTTGGCGACGCTGCTGAAAGCGGGTATC
GCGCTGCTGCAAGCCTTCGACATCATCAGCGAGGGCGTTGAAAACCACCCGCTGCGCGAGCTGGTCAAGGGCTTGAAACA
ACAGGTCGCGTCCGGTAACAGCCTGGCCAGCGCGCTGCGCCAACACCCCCGTTACTTCGATGACCTGTACTGCCACCTGG
TTGCCGCCGGTGAACAGGCCGGCGCCCTGGAAACGCTGCTGGAGCGCGTGGCCGTTCATCTGGAAAAAAGCCAACAGCTC
AAGACCAGAATCAAGAAAGCCATGATCTACCCGCTGGCGGTGCTGGTGGTGGCCGGCCTGGTCAGCGCAGTATTGTTGAT
GCAGGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGAGTCGACACCCCATTGCCCGGTTTTACCCTGGGGGTCATCGCCC
TCTCCGATTTCCTGCGACAGGCCTGGTGGCTACTGGCGCTCGGCCTGGGCGCAGCCATCGGCGGGCTGCGCCACGCCTAT
CGTGTTTCTGCGCAGTTTCGCTACCGGCTGGAGGCGGGTTTGTTGAAAGCCCCCCTGGCAGGCAAACTATCGAGCAAAGC
TGCCGTCGCCCGCTACGCCCGTACGCTTTCAACCACCTTTGCGGCAGGCGTTCCCCTGGTGCAGGCTCTGGATTCAGTGG
CGGGCGCCGTTGGTAATGGCCCTTTCAGACAGGCAATCGAACATATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCCATGGCCACCAGCGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCAATCGGCGAAGAATCCGGCACCCTGGA
CTACATGCTGGAAAAGGTCGCAAACCATTACGAGGCCGACGTCGAGACCCTCGTCGATAACCTCACCAGCTTGATGGAAC
CGCTGATCATGCTGGTGCTGGGGAGCATCGTCGGCGCGCTGGTGATTGCAATGTACCTGCCGGTGTTTCAACTGGGTTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.186

98.756

0.575
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Acinetobacter baumannii D1279779

49.62

98.259

0.488
  pilC Legionella pneumophila strain ERS1305867

47.342

98.259

0.465
  pilG Neisseria gonorrhoeae MS11

40.42

94.776

0.383
  pilG Neisseria meningitidis 44/76-A

40.157

94.776

0.381
  pilC Vibrio campbellii strain DS40M4

38

99.502

0.378
  pilC Thermus thermophilus HB27

38.095

99.254

0.378
  pilC Vibrio cholerae strain A1552

38.325

98.01

0.376

Multiple sequence alignment