Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4J96_RS03885 Genome accession   NZ_CP027725
Coordinates   886161..887369 (-) Length   402 a.a.
NCBI ID   WP_124425977.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain R2-66-08W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 881161..892369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J96_RS03845 (C4J96_0785) - 881171..882469 (-) 1299 WP_124425975.1 NAD(P)/FAD-dependent oxidoreductase -
  C4J96_RS03850 (C4J96_0786) - 882530..882703 (-) 174 WP_124356994.1 DUF3094 family protein -
  C4J96_RS03855 (C4J96_0787) - 882757..883386 (-) 630 WP_124356995.1 DUF1780 domain-containing protein -
  C4J96_RS03860 (C4J96_0788) - 883516..884205 (+) 690 WP_124356996.1 energy-coupling factor ABC transporter permease -
  C4J96_RS03865 (C4J96_0789) - 884269..884484 (+) 216 WP_003171680.1 hypothetical protein -
  C4J96_RS03870 (C4J96_0790) yacG 884481..884681 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  C4J96_RS03875 (C4J96_0791) coaE 884678..885301 (-) 624 WP_124425976.1 dephospho-CoA kinase -
  C4J96_RS03880 (C4J96_0792) pilD 885298..886164 (-) 867 WP_124356998.1 prepilin peptidase Machinery gene
  C4J96_RS03885 (C4J96_0793) pilC 886161..887369 (-) 1209 WP_124425977.1 type II secretion system F family protein Machinery gene
  C4J96_RS03890 (C4J96_0794) - 887568..887981 (+) 414 WP_124360463.1 pilin -
  C4J96_RS03900 (C4J96_0796) - 888325..888630 (+) 306 WP_124357000.1 DUF6388 family protein -
  C4J96_RS03905 (C4J96_0797) nadC 888751..889599 (-) 849 WP_124357001.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4J96_RS03910 (C4J96_0799) - 889840..891996 (+) 2157 WP_124425978.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43268.64 Da        Isoelectric Point: 9.7366

>NTDB_id=279426 C4J96_RS03885 WP_124425977.1 886161..887369(-) (pilC) [Pseudomonas orientalis strain R2-66-08W]
MNDSSTIYAWQGINRKGRRVCGKIAGHDLALVKAQLRAQGICPERVRRQSPGLPSLAAPIRPADIALFTRQLATLLKAGI
ALLQAFDIISEGVENHPLRELVKGLKQQVASGNSLASALRQHPRYFDDLYCHLVAAGEQAGALETLLERVAVHLEKSQQL
KTRIKKAMIYPLAVLVVASLVSAVLLMQVVPQFQNLFAGVDTPLPGFTLGVIALSDFLRQAWWLLALGLGAAIGGLRHAY
RVSAQFRYRLEAGLLKVPLAGKLSSKAAVARYARTLSTTFAAGVPLVQALDSVAGAVGNGPFRQAIEHMRHDVSTGMQLN
QSMATSGLFPGMAIQMTAIGEESGTLDYMLEKVANHYEADVETLVDNLTSLMEPLIMLVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=279426 C4J96_RS03885 WP_124425977.1 886161..887369(-) (pilC) [Pseudomonas orientalis strain R2-66-08W]
ATGAACGACAGCTCGACGATCTACGCCTGGCAAGGCATCAACCGCAAAGGTCGCAGGGTGTGTGGGAAAATCGCAGGGCA
TGACCTTGCGTTGGTCAAGGCACAACTGCGCGCGCAAGGGATCTGCCCCGAGCGTGTGCGCAGGCAATCGCCAGGACTGC
CAAGCCTGGCTGCGCCGATACGGCCAGCGGACATCGCCCTGTTCACCCGCCAGTTGGCGACGCTGCTGAAAGCGGGTATC
GCGCTGCTGCAAGCCTTCGACATCATCAGCGAGGGCGTTGAAAACCACCCGCTGCGCGAGCTGGTCAAGGGCTTGAAACA
ACAGGTCGCGTCCGGTAACAGCCTGGCCAGCGCGCTGCGCCAACACCCCCGTTACTTCGATGACCTGTACTGCCACCTGG
TTGCCGCCGGTGAACAGGCCGGCGCCCTGGAAACGCTGCTGGAGCGCGTGGCCGTTCATCTGGAAAAAAGCCAACAGCTC
AAGACCAGAATCAAGAAAGCCATGATCTACCCGCTGGCGGTGCTGGTGGTGGCCAGCCTGGTCAGCGCAGTGTTGTTGAT
GCAGGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGAGTCGACACCCCATTGCCCGGTTTTACCCTGGGGGTCATCGCCC
TCTCCGATTTCCTGCGACAGGCCTGGTGGCTACTGGCGCTCGGCCTGGGCGCAGCCATCGGCGGGCTGCGCCACGCCTAT
CGTGTTTCTGCGCAGTTTCGCTACCGGCTGGAGGCGGGTTTGTTGAAAGTCCCCCTGGCAGGCAAACTATCGAGCAAAGC
TGCCGTCGCCCGCTACGCCCGTACGCTTTCAACCACCTTTGCGGCAGGCGTTCCCCTGGTGCAGGCTCTGGATTCAGTGG
CGGGCGCCGTTGGTAATGGCCCTTTCAGACAGGCAATCGAACATATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCCATGGCCACCAGCGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCAATCGGCGAAGAATCCGGCACCCTGGA
CTACATGCTGGAAAAGGTCGCAAACCATTACGAGGCCGACGTCGAGACCCTCGTCGATAACCTCACCAGCTTGATGGAAC
CGCTGATCATGCTGGTGCTGGGGAGCATCGTCGGCGCGCTGGTGATTGCAATGTACCTGCCGGTGTTTCAACTGGGTTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.438

98.756

0.577
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Acinetobacter baumannii D1279779

49.62

98.259

0.488
  pilC Legionella pneumophila strain ERS1305867

47.342

98.259

0.465
  pilG Neisseria gonorrhoeae MS11

40.42

94.776

0.383
  pilC Vibrio campbellii strain DS40M4

38.25

99.502

0.381
  pilG Neisseria meningitidis 44/76-A

40.157

94.776

0.381
  pilC Thermus thermophilus HB27

38.095

99.254

0.378
  pilC Vibrio cholerae strain A1552

38.325

98.01

0.376

Multiple sequence alignment