Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4J98_RS03880 Genome accession   NZ_CP027723
Coordinates   873052..874260 (-) Length   402 a.a.
NCBI ID   WP_124431458.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain 8B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 868052..879260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J98_RS03840 (C4J98_0768) - 868062..869360 (-) 1299 WP_124431453.1 NAD(P)/FAD-dependent oxidoreductase -
  C4J98_RS03845 (C4J98_0769) - 869421..869594 (-) 174 WP_065887994.1 DUF3094 family protein -
  C4J98_RS03850 (C4J98_0770) - 869648..870277 (-) 630 WP_124431454.1 DUF1780 domain-containing protein -
  C4J98_RS03855 (C4J98_0771) - 870407..871096 (+) 690 WP_124431455.1 energy-coupling factor ABC transporter permease -
  C4J98_RS03860 (C4J98_0772) - 871160..871375 (+) 216 WP_003171680.1 hypothetical protein -
  C4J98_RS03865 (C4J98_0773) yacG 871372..871572 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  C4J98_RS03870 (C4J98_0774) coaE 871569..872192 (-) 624 WP_124431456.1 dephospho-CoA kinase -
  C4J98_RS03875 (C4J98_0775) pilD 872189..873055 (-) 867 WP_124431457.1 prepilin peptidase Machinery gene
  C4J98_RS03880 (C4J98_0776) pilC 873052..874260 (-) 1209 WP_124431458.1 type II secretion system F family protein Machinery gene
  C4J98_RS03885 (C4J98_0777) - 874459..874872 (+) 414 WP_124425575.1 pilin -
  C4J98_RS03895 (C4J98_0779) - 875174..875479 (+) 306 WP_124431459.1 DUF6388 family protein -
  C4J98_RS03900 (C4J98_0780) nadC 875600..876448 (-) 849 WP_124431460.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4J98_RS03905 (C4J98_0782) - 876692..878848 (+) 2157 WP_124431461.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43359.79 Da        Isoelectric Point: 9.8465

>NTDB_id=279378 C4J98_RS03880 WP_124431458.1 873052..874260(-) (pilC) [Pseudomonas orientalis strain 8B]
MNDSSTIYAWQGIDRKGRRVCGKIAGHDLALVKAQLREQGICPERVRRQSPGLPSLAAPIRPADIALFTRQLATLLKASI
ALLQAFDIISEGVENRPLRELVKGLKQQVASGNSLTSALRQYPRYFDELYCHLVAAGEQAGALETLLERVAVHLEKSQQL
KTRIKKAMIYPLAVLVVAGLVSAVLLMQVVPQFQNLFAGVDSPLPGFTLGVIALSDFLRQAWWLLALGLGVAIGGLRHAY
RVSAGFRYRLETGLLKVPLAGKLSNKAAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIEHMRHDVSTGMQLN
QSMAASGLFPRMAIQMTAIGEESGTLDHMLEKVANHYEADVETLVDNLTSLMEPLIMVVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=279378 C4J98_RS03880 WP_124431458.1 873052..874260(-) (pilC) [Pseudomonas orientalis strain 8B]
ATGAACGACAGCTCGACGATCTACGCCTGGCAAGGCATCGACCGCAAAGGCCGCAGGGTGTGCGGGAAAATCGCAGGGCA
TGACCTTGCGTTGGTCAAGGCACAACTGCGCGAGCAAGGGATCTGCCCCGAGCGTGTGCGCAGGCAATCGCCAGGCCTGC
CAAGCCTGGCTGCGCCGATACGGCCGGCGGACATCGCCCTGTTCACCCGCCAGCTGGCGACGCTGTTGAAGGCGAGTATC
GCGCTGCTGCAAGCCTTCGACATCATCAGCGAGGGCGTTGAAAACCGCCCGCTGCGCGAGCTGGTCAAGGGCTTGAAACA
ACAGGTCGCGTCCGGTAACAGCCTGACCAGCGCGCTGCGCCAATACCCTCGTTACTTCGACGAGCTGTACTGCCACCTGG
TTGCCGCCGGTGAACAGGCCGGCGCCCTGGAAACGCTGCTGGAGCGCGTAGCCGTTCATCTGGAAAAAAGCCAACAACTC
AAGACCAGAATCAAGAAAGCCATGATCTACCCGCTTGCGGTGCTGGTGGTGGCCGGTCTGGTCAGCGCAGTGTTGTTGAT
GCAGGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGAGTCGACAGCCCATTGCCCGGTTTTACCCTGGGGGTCATCGCCC
TCTCCGATTTTCTGCGACAGGCCTGGTGGCTACTGGCGCTCGGCCTGGGCGTGGCCATTGGCGGGCTGCGCCACGCCTAC
CGTGTTTCTGCGGGTTTTCGCTACCGACTGGAAACGGGTTTGTTGAAAGTGCCCCTGGCAGGCAAACTATCGAACAAAGC
TGCCGTCGCCCGCTACGCCCGTACGCTTTCAACCACCTTTGCGGCAGGCGTTCCCCTGGTGCAGGCGCTGGGCTCAGTGG
CCGGTGCCGTCGGTAACGGCCCTTTCAAACAGGCAATCGAACATATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCCATGGCCGCCAGCGGCCTGTTTCCACGCATGGCGATCCAGATGACGGCTATCGGCGAAGAATCCGGCACCCTGGA
CCACATGCTGGAAAAAGTTGCGAACCATTACGAGGCCGACGTAGAGACCCTCGTCGATAACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTGCTGGGGAGCATCGTCGGCGCGCTGGTGATTGCAATGTACCTGCCGGTGTTTCAACTGGGTTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.179

98.756

0.565
  pilC Acinetobacter baylyi ADP1

51.515

98.507

0.507
  pilC Acinetobacter baumannii D1279779

49.367

98.259

0.485
  pilC Legionella pneumophila strain ERS1305867

46.582

98.259

0.458
  pilC Thermus thermophilus HB27

38.155

99.751

0.381
  pilG Neisseria gonorrhoeae MS11

39.895

94.776

0.378
  pilC Vibrio cholerae strain A1552

38.131

98.507

0.376
  pilC Vibrio campbellii strain DS40M4

37.5

99.502

0.373
  pilG Neisseria meningitidis 44/76-A

39.108

94.776

0.371

Multiple sequence alignment