Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ASA_RS18035 Genome accession   NC_009348
Coordinates   3922376..3923485 (+) Length   369 a.a.
NCBI ID   WP_005320465.1    Uniprot ID   A2T1B0
Organism   Aeromonas salmonicida subsp. salmonicida A449     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3917376..3928485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASA_RS18000 (ASA_3626) - 3917965..3918264 (+) 300 WP_011899099.1 hypothetical protein -
  ASA_RS18005 (ASA_3627) - 3918333..3918752 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  ASA_RS18010 (ASA_3628) yggU 3918771..3919070 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  ASA_RS18015 (ASA_3629) - 3919070..3919621 (-) 552 WP_005320477.1 YggT family protein -
  ASA_RS18020 (ASA_3630) proC 3919650..3920474 (-) 825 WP_005320475.1 pyrroline-5-carboxylate reductase -
  ASA_RS18025 (ASA_3631) - 3920565..3921266 (-) 702 WP_005320467.1 YggS family pyridoxal phosphate-dependent enzyme -
  ASA_RS18030 (ASA_3632) pilT 3921306..3922340 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ASA_RS18035 (ASA_3633) pilU 3922376..3923485 (+) 1110 WP_005320465.1 type IVa pilus ATPase TapU Machinery gene
  ASA_RS18040 (ASA_3634) yaaA 3923517..3924290 (+) 774 WP_005320464.1 peroxide stress protein YaaA -
  ASA_RS18045 (ASA_3635) srmB 3924479..3925702 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  ASA_RS18050 (ASA_3636) - 3925831..3926541 (+) 711 WP_011899100.1 methyltransferase -
  ASA_RS18055 (ASA_3637) brnQ 3926727..3928040 (+) 1314 WP_017412062.1 branched-chain amino acid transport system II carrier protein -
  ASA_RS18060 (ASA_3638) - 3928116..3928346 (-) 231 WP_005320453.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41039.07 Da        Isoelectric Point: 6.3717

>NTDB_id=27912 ASA_RS18035 WP_005320465.1 3922376..3923485(+) (pilU) [Aeromonas salmonicida subsp. salmonicida A449]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLVSLGSEALDKQSALTLVRETLSPEHFERYIRTREGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=27912 ASA_RS18035 WP_005320465.1 3922376..3923485(+) (pilU) [Aeromonas salmonicida subsp. salmonicida A449]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGGACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGGTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAGA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAGGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGTCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCCCCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGCCTAGCGACCCTTCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTGCCGCAGGAGAAACACCGTCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGCCGCCGCCGCTTCGCGGCGTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGCCTCAAAGAGGTGATGACCAAATCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T1B0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.511

99.729

0.593
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36

Multiple sequence alignment