Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   SPYM_RS00645 Genome accession   NC_009332
Coordinates   103877..104815 (+) Length   312 a.a.
NCBI ID   WP_011888534.1    Uniprot ID   -
Organism   Streptococcus pyogenes str. Manfredo     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98877..109815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM_RS00635 (SpyM50082) rpoC 99626..103267 (+) 3642 WP_011888533.1 DNA-directed RNA polymerase subunit beta' -
  SPYM_RS00640 (SpyM50083) - 103419..103784 (+) 366 WP_002986560.1 DUF1033 family protein -
  SPYM_RS00645 (SpyM50084) comYA 103877..104815 (+) 939 WP_011888534.1 competence type IV pilus ATPase ComGA Machinery gene
  SPYM_RS00650 (SpyM50085) comYB 104751..105785 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPYM_RS00655 (SpyM50086) comYC 105787..106113 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  SPYM_RS00660 (SpyM50087) comGD 106088..106516 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SPYM_RS00665 (SpyM50088) comGE 106473..106757 (+) 285 WP_041174183.1 competence type IV pilus minor pilin ComGE -
  SPYM_RS00670 (SpyM50089) comYF 106750..107184 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPYM_RS00675 (SpyM50090) comGG 107168..107494 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  SPYM_RS00680 (SpyM50091) comYH 107592..108545 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  SPYM_RS00685 (SpyM50092) - 108604..109800 (+) 1197 WP_011888536.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35101.38 Da        Isoelectric Point: 8.5799

>NTDB_id=27834 SPYM_RS00645 WP_011888534.1 103877..104815(+) (comYA) [Streptococcus pyogenes str. Manfredo]
MVQVLAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=27834 SPYM_RS00645 WP_011888534.1 103877..104815(+) (comYA) [Streptococcus pyogenes str. Manfredo]
ATGGTACAAGTATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTATCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.521

99.679

0.583
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

40

92.949

0.372

Multiple sequence alignment