Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   SPYM_RS00670 Genome accession   NC_009332
Coordinates   106750..107184 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes str. Manfredo     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101750..112184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM_RS00640 (SpyM50083) - 103419..103784 (+) 366 WP_002986560.1 DUF1033 family protein -
  SPYM_RS00645 (SpyM50084) comYA 103877..104815 (+) 939 WP_011888534.1 competence type IV pilus ATPase ComGA Machinery gene
  SPYM_RS00650 (SpyM50085) comYB 104751..105785 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPYM_RS00655 (SpyM50086) comYC 105787..106113 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  SPYM_RS00660 (SpyM50087) comGD 106088..106516 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SPYM_RS00665 (SpyM50088) comGE 106473..106757 (+) 285 WP_041174183.1 competence type IV pilus minor pilin ComGE -
  SPYM_RS00670 (SpyM50089) comYF 106750..107184 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPYM_RS00675 (SpyM50090) comGG 107168..107494 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  SPYM_RS00680 (SpyM50091) comYH 107592..108545 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  SPYM_RS00685 (SpyM50092) - 108604..109800 (+) 1197 WP_011888536.1 acetate kinase -
  SPYM_RS00690 (SpyM50093) - 109987..110295 (+) 309 WP_011017251.1 hypothetical protein -
  SPYM_RS00695 (SpyM50094) proC 110378..111148 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=27837 SPYM_RS00670 WP_002986542.1 106750..107184(+) (comYF) [Streptococcus pyogenes str. Manfredo]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=27837 SPYM_RS00670 WP_002986542.1 106750..107184(+) (comYF) [Streptococcus pyogenes str. Manfredo]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment