Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AM474_RS20735 Genome accession   NZ_CP027538
Coordinates   4290327..4291544 (-) Length   405 a.a.
NCBI ID   WP_003116250.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_0095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4285327..4296544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM474_RS20690 (AM474_20690) - 4285509..4285958 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  AM474_RS20695 (AM474_20695) - 4285955..4286584 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  AM474_RS20700 (AM474_20700) - 4286717..4287142 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  AM474_RS20705 (AM474_20705) - 4287139..4287609 (+) 471 WP_003094664.1 hypothetical protein -
  AM474_RS20710 (AM474_20710) - 4287606..4288295 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  AM474_RS20715 (AM474_20715) - 4288401..4288610 (+) 210 WP_003094660.1 hypothetical protein -
  AM474_RS20720 (AM474_20720) yacG 4288647..4288847 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  AM474_RS20725 (AM474_20725) coaE 4288844..4289455 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  AM474_RS20730 (AM474_20730) pilD 4289452..4290324 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AM474_RS20735 (AM474_20735) pilC 4290327..4291544 (-) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  AM474_RS20740 (AM474_20740) pilB 4291548..4293251 (-) 1704 WP_034027601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AM474_RS20745 (AM474_20745) - 4293480..4294019 (+) 540 WP_033938465.1 pilin -
  AM474_RS20750 (AM474_20750) - 4294083..4294781 (+) 699 WP_227788634.1 hypothetical protein -
  AM474_RS20760 (AM474_20760) nadC 4294945..4295793 (-) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44051.94 Da        Isoelectric Point: 9.8417

>NTDB_id=277869 AM474_RS20735 WP_003116250.1 4290327..4291544(-) (pilC) [Pseudomonas aeruginosa strain AR_0095]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQEWWLIVLIGLFAAAFAFR
EVHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=277869 AM474_RS20735 WP_003116250.1 4290327..4291544(-) (pilC) [Pseudomonas aeruginosa strain AR_0095]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTAGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAGGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTGTCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGCGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATTCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGTTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment