Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AM474_RS20740 Genome accession   NZ_CP027538
Coordinates   4291548..4293251 (-) Length   567 a.a.
NCBI ID   WP_034027601.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_0095     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4286548..4298251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM474_RS20700 (AM474_20700) - 4286717..4287142 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  AM474_RS20705 (AM474_20705) - 4287139..4287609 (+) 471 WP_003094664.1 hypothetical protein -
  AM474_RS20710 (AM474_20710) - 4287606..4288295 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  AM474_RS20715 (AM474_20715) - 4288401..4288610 (+) 210 WP_003094660.1 hypothetical protein -
  AM474_RS20720 (AM474_20720) yacG 4288647..4288847 (-) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  AM474_RS20725 (AM474_20725) coaE 4288844..4289455 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  AM474_RS20730 (AM474_20730) pilD 4289452..4290324 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AM474_RS20735 (AM474_20735) pilC 4290327..4291544 (-) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  AM474_RS20740 (AM474_20740) pilB 4291548..4293251 (-) 1704 WP_034027601.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AM474_RS20745 (AM474_20745) - 4293480..4294019 (+) 540 WP_033938465.1 pilin -
  AM474_RS20750 (AM474_20750) - 4294083..4294781 (+) 699 WP_227788634.1 hypothetical protein -
  AM474_RS20760 (AM474_20760) nadC 4294945..4295793 (-) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62624.61 Da        Isoelectric Point: 5.7065

>NTDB_id=277870 AM474_RS20740 WP_034027601.1 4291548..4293251(-) (pilB) [Pseudomonas aeruginosa strain AR_0095]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPAKHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=277870 AM474_RS20740 WP_034027601.1 4291548..4293251(-) (pilB) [Pseudomonas aeruginosa strain AR_0095]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAAAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAAGCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Acinetobacter baylyi ADP1

57.092

99.471

0.568
  pilB Legionella pneumophila strain ERS1305867

55.282

100

0.554
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.763

95.062

0.492
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.647

95.414

0.483
  pilF Thermus thermophilus HB27

38.407

99.647

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

93.474

0.374

Multiple sequence alignment