Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHAL_RS10205 Genome accession   NC_008789
Coordinates   2223153..2224313 (+) Length   386 a.a.
NCBI ID   WP_011814806.1    Uniprot ID   A1WYM2
Organism   Halorhodospira halophila SL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2218153..2229313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHAL_RS10180 (Hhal_2015) - 2218850..2220142 (+) 1293 WP_011814801.1 DEAD/DEAH box helicase -
  HHAL_RS10185 (Hhal_2016) - 2220231..2220665 (+) 435 WP_011814802.1 CBS domain-containing protein -
  HHAL_RS10190 (Hhal_2017) - 2220686..2221591 (+) 906 WP_011814803.1 MotA/TolQ/ExbB proton channel family protein -
  HHAL_RS10195 (Hhal_2018) - 2221607..2222335 (+) 729 WP_011814804.1 OmpA family protein -
  HHAL_RS10200 (Hhal_2019) - 2222398..2223078 (+) 681 WP_011814805.1 rhomboid family intramembrane serine protease -
  HHAL_RS10205 (Hhal_2020) pilB 2223153..2224313 (+) 1161 WP_011814806.1 GspE/PulE family protein Machinery gene
  HHAL_RS10210 (Hhal_2021) - 2224301..2225491 (+) 1191 WP_011814807.1 type II secretion system F family protein -
  HHAL_RS10215 (Hhal_2022) - 2225544..2226398 (+) 855 WP_049751649.1 A24 family peptidase -
  HHAL_RS10220 (Hhal_2023) coaE 2226395..2227027 (+) 633 WP_011814809.1 dephospho-CoA kinase -
  HHAL_RS10225 (Hhal_2024) zapD 2227039..2227824 (+) 786 WP_011814810.1 cell division protein ZapD -
  HHAL_RS10230 (Hhal_2025) - 2227836..2228804 (-) 969 WP_011814811.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 41420.18 Da        Isoelectric Point: 6.6940

>NTDB_id=27348 HHAL_RS10205 WP_011814806.1 2223153..2224313(+) (pilB) [Halorhodospira halophila SL1]
MDDDRATVRLVDRLLADAVRRRASDIHLQPEADRVRVRLRIDGLLREAEGPPPGLRGRVAARIKLLAGMDVAEQRLPQDG
RLEARDGDGQRVQFRVASCPGVHGEKLVLRLIEQDAPATLDALDLPGPARAALESALDRPDGLILVTGPTGSGKTATLHA
ALRRLNTPERNICAVEDPVEMDTPGVNQVAVNRRAGIDFAQALRAFLRQDPDVIMIGEIRDAETAAIAVKAAQTGHLVLS
TLHTRSAPGAVERLAQMGLPGYDLASSLSLVVAQRLVRRLCPACRETSSAAAQPAAAEPAGVYHPRGCPECQDGYRGRRG
VFQVMPMTDAVADAVLHGPSAREIEARARAAGMPDLHDAGWPLVETGETSAAELRRVTREAEPWPA

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=27348 HHAL_RS10205 WP_011814806.1 2223153..2224313(+) (pilB) [Halorhodospira halophila SL1]
ATGGACGACGATCGCGCCACGGTCCGCCTCGTGGACCGTCTTCTTGCCGACGCCGTGCGGCGTCGGGCCTCGGATATCCA
CCTGCAACCGGAGGCCGACCGGGTGCGCGTGCGCCTGCGCATCGACGGCCTGCTGCGCGAGGCCGAAGGGCCACCGCCGG
GCCTGCGCGGACGGGTTGCGGCACGCATCAAACTGCTGGCGGGGATGGACGTCGCCGAGCAACGCCTGCCCCAGGACGGC
CGGCTGGAGGCCCGGGACGGCGACGGGCAGCGGGTGCAGTTCCGCGTCGCCAGCTGCCCGGGCGTCCACGGCGAGAAACT
GGTCCTGCGCCTGATCGAGCAGGACGCCCCGGCCACCCTCGACGCCCTGGACCTCCCCGGCCCGGCCCGGGCGGCACTGG
AGTCGGCCCTCGACCGCCCCGACGGACTGATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCGCGACCCTGCACGCC
GCCCTGCGGCGGCTGAACACCCCCGAGCGCAACATCTGCGCCGTGGAGGATCCCGTGGAAATGGACACCCCCGGGGTCAA
CCAGGTGGCCGTGAACCGCCGCGCCGGTATCGACTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGA
TCATGATCGGCGAGATCCGCGACGCCGAGACCGCCGCCATCGCCGTCAAGGCGGCCCAGACCGGCCACTTGGTCCTCTCA
ACCCTGCATACACGCAGCGCGCCGGGCGCGGTGGAGCGCCTGGCGCAGATGGGTCTGCCCGGCTACGACCTGGCCTCGAG
CCTCTCCCTGGTGGTGGCGCAGCGCCTGGTCCGCCGCCTCTGCCCGGCCTGCCGCGAGACCAGCAGCGCCGCCGCTCAGC
CGGCGGCAGCGGAGCCGGCCGGCGTCTACCACCCCCGCGGGTGCCCGGAGTGCCAGGACGGCTATCGCGGTCGGCGGGGG
GTCTTTCAGGTCATGCCGATGACCGACGCCGTGGCCGATGCCGTCCTGCATGGCCCTTCGGCCCGCGAGATCGAAGCCCG
CGCCCGGGCGGCGGGCATGCCGGATCTCCACGACGCCGGCTGGCCCCTGGTGGAGACCGGCGAGACCAGCGCCGCCGAAC
TACGCCGCGTCACCCGCGAGGCCGAGCCGTGGCCCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1WYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

48.953

98.964

0.484
  pilB Vibrio campbellii strain DS40M4

48.031

98.705

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

46.719

98.705

0.461
  pilB Acinetobacter baumannii D1279779

45.501

100

0.459
  pilB Legionella pneumophila strain ERS1305867

45.337

100

0.453
  pilB Acinetobacter baylyi ADP1

44.845

100

0.451
  pilF Neisseria gonorrhoeae MS11

44.156

99.741

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

41.103

100

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.371

100

0.422
  pilF Thermus thermophilus HB27

42.037

99.223

0.417
  pilB Glaesserella parasuis strain SC1401

47.214

88.342

0.417
  pilB Haemophilus influenzae 86-028NP

41.534

97.927

0.407
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.211

98.446

0.386
  pilB Haemophilus influenzae Rd KW20

45.092

84.456

0.381

Multiple sequence alignment