Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C5F70_RS20410 Genome accession   NZ_CP026880
Coordinates   4418840..4420048 (+) Length   402 a.a.
NCBI ID   WP_017845469.1    Uniprot ID   A0A1D3JRP0
Organism   Pseudomonas sp. LH1G9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4413840..4425048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5F70_RS20385 nadC 4415334..4416182 (+) 849 WP_046384727.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C5F70_RS20390 - 4416226..4416531 (-) 306 WP_017845466.1 DUF6388 family protein -
  C5F70_RS20400 - 4416780..4418189 (-) 1410 WP_104903959.1 O-antigen ligase -
  C5F70_RS20405 - 4418225..4418638 (-) 414 WP_017845468.1 pilin -
  C5F70_RS20410 pilC 4418840..4420048 (+) 1209 WP_017845469.1 type II secretion system F family protein Machinery gene
  C5F70_RS20415 pilD 4420045..4420911 (+) 867 WP_017845470.1 A24 family peptidase Machinery gene
  C5F70_RS20420 coaE 4420908..4421531 (+) 624 WP_017845471.1 dephospho-CoA kinase -
  C5F70_RS20425 yacG 4421528..4421728 (+) 201 WP_017845472.1 DNA gyrase inhibitor YacG -
  C5F70_RS20430 - 4421725..4421940 (-) 216 WP_003171680.1 hypothetical protein -
  C5F70_RS20435 - 4422017..4422706 (-) 690 WP_017845473.1 energy-coupling factor ABC transporter permease -
  C5F70_RS20440 - 4422703..4423170 (-) 468 WP_104903960.1 FAD/FMN-containing dehydrogenase -
  C5F70_RS20445 - 4423270..4423899 (+) 630 WP_017845475.1 DUF1780 domain-containing protein -
  C5F70_RS20450 - 4423990..4424163 (+) 174 WP_016976295.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43430.68 Da        Isoelectric Point: 9.8307

>NTDB_id=273032 C5F70_RS20410 WP_017845469.1 4418840..4420048(+) (pilC) [Pseudomonas sp. LH1G9]
MTNAATTYAWEGINRQGRRVSGQTVSHDLARVKAQLRQQGICPGRVRKQSAGLPSLAAPIKPADIALFTRQLATLLKAGI
ALLQAFDVISEGFDNRHMYRLVKGLKQEIGAGNTLASALRKQPRYFDDLYCNLVAAGEQAGALETLLERVADHLEKSERL
KARIKKAMTYPIAVIVVASLVSSLLLIHVVPQFQNLFASVDSELPGFTLYVIALSEFLQQAWWMLALSLGAGGLGLRRAY
RVSPGFRHWLDAGLLKAPLAGTLLKKSAVARYARTLSTTFAAGVPLVQALDSVAAAAGNQLFKQAIEHMRHDVSTGMPLN
RAMATSGLFPGMAIQMTAIGEESGTLEHMLEKVASHYESDVDTLVDQLTSLMEPLIMVVLGGIVGALVIALYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=273032 C5F70_RS20410 WP_017845469.1 4418840..4420048(+) (pilC) [Pseudomonas sp. LH1G9]
ATGACCAACGCTGCCACGACATACGCTTGGGAAGGCATCAACCGCCAGGGACGCAGGGTGTCCGGGCAAACCGTCAGCCA
CGACCTCGCGCGGGTCAAGGCACAACTGCGCCAGCAAGGGATTTGCCCTGGCCGCGTGCGCAAACAATCCGCCGGCCTGC
CAAGCCTCGCTGCGCCGATCAAACCGGCGGACATTGCCCTGTTCACTCGCCAACTGGCGACACTGCTGAAGGCCGGCATT
GCCTTGCTGCAAGCCTTCGACGTGATCAGCGAAGGCTTCGACAACCGCCACATGTACAGGCTGGTAAAAGGCCTGAAACA
GGAGATCGGCGCCGGAAATACCCTGGCGTCGGCCCTGCGCAAACAGCCGCGCTACTTCGATGACCTGTACTGCAACCTGG
TGGCGGCGGGTGAGCAGGCCGGTGCCCTGGAAACCCTTCTGGAACGGGTGGCGGATCACCTGGAAAAGAGCGAACGGCTC
AAGGCCCGGATCAAGAAGGCCATGACCTACCCCATCGCGGTCATCGTGGTCGCCAGCCTTGTCAGCAGTCTCTTGCTGAT
CCATGTGGTGCCGCAATTCCAGAACCTGTTCGCCAGCGTGGACAGCGAGTTGCCTGGTTTTACTCTGTACGTGATTGCCT
TGTCCGAGTTCCTGCAACAAGCCTGGTGGATGCTGGCGCTCAGCCTGGGCGCGGGAGGTCTGGGGTTGCGCCGGGCCTAT
CGCGTATCGCCGGGTTTTCGTCATTGGCTGGATGCCGGTTTGTTGAAAGCGCCCCTGGCGGGCACACTGCTGAAAAAGTC
GGCGGTCGCCCGCTACGCACGCACGCTTTCAACTACGTTCGCGGCCGGAGTGCCGTTGGTGCAGGCCCTGGATTCAGTGG
CCGCCGCTGCCGGCAACCAGCTATTCAAACAGGCTATCGAACATATGCGTCACGATGTATCCACAGGCATGCCGTTGAAT
CGGGCCATGGCTACCAGCGGTCTGTTCCCTGGCATGGCGATCCAGATGACGGCGATTGGTGAAGAATCCGGCACCCTGGA
GCACATGCTGGAAAAAGTCGCGAGCCACTATGAGTCGGACGTCGACACGCTGGTCGACCAGCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTGCTCGGCGGGATTGTCGGCGCTTTGGTAATCGCCCTGTACCTGCCGGTCTTCCAACTCGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D3JRP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.848

98.507

0.59
  pilC Acinetobacter baylyi ADP1

50.873

99.751

0.507
  pilC Acinetobacter baumannii D1279779

49.242

98.507

0.485
  pilC Legionella pneumophila strain ERS1305867

47.98

98.507

0.473
  pilC Vibrio cholerae strain A1552

39.44

97.761

0.386
  pilC Thermus thermophilus HB27

37.94

99.005

0.376
  pilG Neisseria gonorrhoeae MS11

39.108

94.776

0.371
  pilC Vibrio campbellii strain DS40M4

37.913

97.761

0.371
  pilG Neisseria meningitidis 44/76-A

38.32

94.776

0.363

Multiple sequence alignment