Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4Q28_RS25325 Genome accession   NZ_CP026676
Coordinates   5442257..5443471 (-) Length   404 a.a.
NCBI ID   WP_371919419.1    Uniprot ID   -
Organism   Pseudomonas sp. SWI6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5437257..5448471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4Q28_RS25310 (C4Q28_25310) - 5438898..5440523 (-) 1626 WP_104882280.1 AAA family ATPase -
  C4Q28_RS25315 (C4Q28_25315) aroB 5440539..5441636 (-) 1098 WP_023378228.1 3-dehydroquinate synthase -
  C4Q28_RS25320 (C4Q28_25320) aroK 5441734..5442252 (-) 519 WP_023378227.1 shikimate kinase AroK -
  C4Q28_RS25325 (C4Q28_25325) pilQ 5442257..5443471 (-) 1215 WP_371919419.1 type IV pilus secretin PilQ Machinery gene
  C4Q28_RS25330 (C4Q28_25330) - 5443458..5444405 (-) 948 WP_238546532.1 pilus assembly protein PilP -
  C4Q28_RS25335 (C4Q28_25335) - 5444462..5444998 (-) 537 WP_023378224.1 PilN domain-containing protein -
  C4Q28_RS25340 (C4Q28_25340) pilM 5444986..5445876 (-) 891 WP_104882282.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43833.08 Da        Isoelectric Point: 4.8579

>NTDB_id=271632 C4Q28_RS25325 WP_371919419.1 5442257..5443471(-) (pilQ) [Pseudomonas sp. SWI6]
MAAPDRGEPLSLNFQDVPVRSVLQVMADYAGINLVASDAVQGNITLRLQDVPWDQALELVLRSKGLGRHQEGNVLLVAPA
AELAEQSRGERIGQELEAQLQPLSRALLPIHHAKASELVELLQATLADDGILTGRGSLAVDARTNTLIAHQPADRLAELR
RLVAELDVPLRQVAIEARIVEANVDYEKSLGVRWGGPLYGETAQLGRDLFVDLGVDRATSGVGLGLLRAEVLLDLELSAM
EKSGNGEIISQPKVVTGDKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDGKVIMAVRVTKDEPDYVNA
LNDVPPIRKNEVNARVRVADGETIVIGGVYSTTQNKVIDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAI
AVSR

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=271632 C4Q28_RS25325 WP_371919419.1 5442257..5443471(-) (pilQ) [Pseudomonas sp. SWI6]
ATGGCGGCGCCTGACCGGGGGGAGCCGCTGTCGCTGAATTTCCAGGATGTGCCCGTGCGCTCAGTGTTGCAGGTGATGGC
CGACTACGCGGGCATCAACCTGGTAGCCAGCGATGCCGTGCAAGGCAACATCACGTTGAGATTGCAGGATGTGCCGTGGG
ATCAGGCGCTGGAGCTGGTGTTGCGCAGCAAGGGGCTGGGCCGTCACCAAGAGGGCAATGTGTTGCTTGTGGCGCCGGCC
GCCGAGTTGGCCGAGCAGTCCCGGGGCGAGCGGATCGGGCAGGAGCTGGAGGCTCAGTTGCAGCCCCTGAGCCGGGCATT
GCTGCCCATCCACCATGCCAAGGCTTCTGAACTTGTCGAGTTGCTCCAGGCGACCCTGGCGGACGACGGTATTCTGACCG
GTCGCGGCAGCCTGGCGGTCGATGCGCGTACCAACACGCTGATCGCCCACCAGCCAGCCGATAGGCTGGCCGAATTGCGC
AGATTGGTGGCCGAACTGGACGTGCCGCTGCGGCAAGTGGCGATCGAGGCGCGCATCGTCGAGGCCAATGTCGACTACGA
AAAGAGCCTGGGCGTGCGCTGGGGTGGACCGCTGTATGGCGAAACGGCGCAGTTGGGTCGTGATTTGTTCGTTGACTTGG
GGGTAGACAGGGCGACCTCCGGGGTGGGGTTGGGCTTGCTGCGCGCGGAAGTGCTGCTGGATCTTGAGCTCAGTGCCATG
GAGAAAAGCGGCAACGGCGAAATCATCTCGCAGCCCAAGGTGGTGACGGGCGACAAGGAAACCGCCAGAATCCTCAAAGG
CACCGAAGTGCCATATCAGGAGACCAGCAAGAGCGGCGCCACGTCTGTCGCTTTCCGCGAGGCATCGTTGTCCCTCGAGG
TCACGCCGCAGATCACCCCAGACGGCAAGGTGATCATGGCAGTCAGAGTGACCAAGGACGAGCCGGACTACGTCAACGCC
TTGAACGACGTGCCTCCGATCCGCAAGAACGAGGTCAATGCCAGGGTTCGTGTAGCTGATGGCGAAACGATAGTGATCGG
TGGAGTCTATTCGACCACGCAAAACAAAGTGATCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGT
TTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATT
GCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(239-395)

Secretin and TonB N terminus short domain (STN)

(33-79)

Bacterial type II/III secretion system short domain (Secretin_N)

(107-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.447

100

0.639
  pilQ Acinetobacter baumannii D1279779

40.132

100

0.453
  pilQ Acinetobacter baumannii strain A118

40.132

100

0.453
  comQ Acinetobacter baylyi ADP1

40.94

100

0.453
  pilQ Legionella pneumophila strain Lp02

42.548

100

0.438
  pilQ Legionella pneumophila strain ERS1305867

42.548

100

0.438
  pilQ Vibrio campbellii strain DS40M4

39.954

100

0.428
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.964

100

0.421
  pilQ Vibrio cholerae strain A1552

40.964

100

0.421
  pilQ Neisseria gonorrhoeae strain FA1090

35.577

100

0.366

Multiple sequence alignment