Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   SHEWANA3_RS02220 Genome accession   NC_008577
Coordinates   475431..476702 (-) Length   423 a.a.
NCBI ID   WP_011715632.1    Uniprot ID   A0KS92
Organism   Shewanella sp. ANA-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470431..481702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWANA3_RS02185 (Shewana3_0411) - 470567..471493 (+) 927 WP_011715626.1 exopolyphosphatase -
  SHEWANA3_RS02190 (Shewana3_0412) - 471568..472104 (-) 537 WP_011715627.1 thioredoxin family protein -
  SHEWANA3_RS02195 (Shewana3_0413) mutT 472252..472650 (-) 399 WP_011715628.1 8-oxo-dGTP diphosphatase MutT -
  SHEWANA3_RS02200 (Shewana3_0414) yacG 472685..472894 (-) 210 WP_011621218.1 DNA gyrase inhibitor YacG -
  SHEWANA3_RS02205 (Shewana3_0415) zapD 473002..473736 (-) 735 WP_011715629.1 cell division protein ZapD -
  SHEWANA3_RS02210 (Shewana3_0416) coaE 473729..474346 (-) 618 WP_011715630.1 dephospho-CoA kinase -
  SHEWANA3_RS02215 (Shewana3_0417) pilD 474440..475366 (-) 927 WP_041412917.1 A24 family peptidase Machinery gene
  SHEWANA3_RS02220 (Shewana3_0418) pilC 475431..476702 (-) 1272 WP_011715632.1 type II secretion system F family protein Machinery gene
  SHEWANA3_RS02225 (Shewana3_0419) pilB 476825..478534 (-) 1710 WP_011715633.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SHEWANA3_RS23550 - 479352..480278 (+) 927 WP_166485342.1 O-antigen ligase family protein -
  SHEWANA3_RS02235 (Shewana3_0421) - 480325..480753 (-) 429 WP_011715635.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 46014.19 Da        Isoelectric Point: 10.2290

>NTDB_id=26866 SHEWANA3_RS02220 WP_011715632.1 475431..476702(-) (pilC) [Shewanella sp. ANA-3]
MATATTVKKPRAKKSEQKSQPKIYTFEWKGLNRDGQKTSGELRGASAAEIRSQLKSQGVNPKTVRKQSAALFKLGDAKIS
PMDIAMITRQIATMLAAGVPLVTTIELLGRGHEKAKMRELLGSILSEIQSGIPLSDSLRPHRRYFDDLYVDLVAAGEHSG
SLDVVFDRIATYREKSEALKSKIKKAMFYPAAVVVVAILVTTLLLLFVVPQFEEIFKGFGAELPAFTQLVLHISRGLQSS
WYIFLGAIVAGVFLFVRAHRNSQMVRDRVDEAVLKIPAIGPILHKGAMARFARTLATTFAAGVPLIDGLESAAGASGNAV
YRKAILKIRQEVMAGMQMNVAMRTTGLFPDMLIQMVMIGEESGSLDNMLNKVANIYEMQVDDAVDGLSSLIEPIMMVVIG
TVVGGLIVAMYLPIFQMGKVVGG

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=26866 SHEWANA3_RS02220 WP_011715632.1 475431..476702(-) (pilC) [Shewanella sp. ANA-3]
ATGGCTACGGCAACTACTGTAAAAAAGCCTCGTGCCAAAAAGAGCGAGCAAAAAAGCCAACCCAAAATCTATACCTTCGA
ATGGAAAGGCCTTAATCGCGACGGTCAGAAAACCAGTGGTGAACTGAGGGGGGCCTCTGCGGCCGAAATTCGCAGCCAAC
TCAAATCCCAAGGTGTAAACCCTAAAACCGTACGCAAGCAATCAGCGGCCCTATTTAAGCTAGGTGACGCAAAAATTAGT
CCAATGGATATTGCCATGATTACACGGCAAATTGCGACAATGCTTGCTGCTGGCGTTCCCTTAGTCACGACTATTGAGTT
ACTCGGTCGCGGTCATGAAAAAGCAAAAATGCGTGAGCTATTAGGCTCCATTCTTTCCGAAATCCAATCGGGTATTCCAC
TATCAGACTCACTACGCCCCCATAGACGCTATTTCGATGATCTCTATGTTGATTTAGTAGCTGCGGGTGAACACTCGGGA
TCTTTGGACGTAGTATTCGACCGTATTGCGACATACCGTGAAAAGTCTGAAGCATTGAAATCTAAGATAAAAAAAGCCAT
GTTTTATCCGGCTGCAGTAGTCGTCGTAGCCATTTTAGTAACAACTCTACTGTTACTCTTTGTTGTCCCGCAGTTTGAAG
AGATTTTTAAAGGATTTGGCGCGGAATTACCTGCTTTTACTCAGTTGGTGCTTCATATATCCAGAGGCTTACAATCCTCT
TGGTATATTTTTTTGGGAGCTATCGTAGCTGGAGTATTTTTGTTTGTTCGCGCCCATAGAAACTCGCAAATGGTTAGAGA
TCGAGTGGATGAAGCCGTATTAAAAATTCCTGCAATTGGACCTATCTTGCATAAAGGTGCAATGGCGCGTTTTGCTCGAA
CCTTAGCCACGACTTTTGCCGCAGGGGTTCCGTTAATCGATGGTTTAGAATCTGCAGCTGGTGCATCAGGAAACGCTGTA
TATCGAAAAGCCATTTTGAAAATCCGTCAAGAGGTGATGGCCGGGATGCAAATGAACGTCGCCATGCGTACCACAGGGCT
TTTCCCCGATATGTTGATCCAAATGGTGATGATCGGTGAAGAATCAGGTTCACTCGATAATATGCTAAACAAAGTAGCCA
ATATTTATGAGATGCAGGTCGATGATGCGGTTGATGGCTTATCAAGCCTTATCGAGCCCATTATGATGGTGGTTATTGGC
ACGGTTGTTGGCGGCCTCATTGTCGCTATGTACTTACCAATCTTCCAAATGGGTAAAGTTGTAGGTGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(88-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KS92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.556

93.617

0.52
  pilC Legionella pneumophila strain ERS1305867

50

95.508

0.478
  pilC Acinetobacter baylyi ADP1

50.63

93.853

0.475
  pilC Acinetobacter baumannii D1279779

49.749

94.09

0.468
  pilC Vibrio cholerae strain A1552

47.619

94.326

0.449
  pilC Vibrio campbellii strain DS40M4

45.185

95.745

0.433
  pilG Neisseria meningitidis 44/76-A

41.542

95.035

0.395
  pilG Neisseria gonorrhoeae MS11

41.542

95.035

0.395

Multiple sequence alignment