Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SHEWANA3_RS02225 Genome accession   NC_008577
Coordinates   476825..478534 (-) Length   569 a.a.
NCBI ID   WP_011715633.1    Uniprot ID   A0KS93
Organism   Shewanella sp. ANA-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471825..483534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWANA3_RS02195 (Shewana3_0413) mutT 472252..472650 (-) 399 WP_011715628.1 8-oxo-dGTP diphosphatase MutT -
  SHEWANA3_RS02200 (Shewana3_0414) yacG 472685..472894 (-) 210 WP_011621218.1 DNA gyrase inhibitor YacG -
  SHEWANA3_RS02205 (Shewana3_0415) zapD 473002..473736 (-) 735 WP_011715629.1 cell division protein ZapD -
  SHEWANA3_RS02210 (Shewana3_0416) coaE 473729..474346 (-) 618 WP_011715630.1 dephospho-CoA kinase -
  SHEWANA3_RS02215 (Shewana3_0417) pilD 474440..475366 (-) 927 WP_041412917.1 A24 family peptidase Machinery gene
  SHEWANA3_RS02220 (Shewana3_0418) pilC 475431..476702 (-) 1272 WP_011715632.1 type II secretion system F family protein Machinery gene
  SHEWANA3_RS02225 (Shewana3_0419) pilB 476825..478534 (-) 1710 WP_011715633.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SHEWANA3_RS23550 - 479352..480278 (+) 927 WP_166485342.1 O-antigen ligase family protein -
  SHEWANA3_RS02235 (Shewana3_0421) - 480325..480753 (-) 429 WP_011715635.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SHEWANA3_RS02240 (Shewana3_0422) nadC 481358..482239 (-) 882 WP_011715636.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SHEWANA3_RS02245 (Shewana3_0423) ampD 482516..483061 (+) 546 WP_011715637.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62776.15 Da        Isoelectric Point: 5.7404

>NTDB_id=26867 SHEWANA3_RS02225 WP_011715633.1 476825..478534(-) (pilB) [Shewanella sp. ANA-3]
MPSAGLHLGLSTLFIRKGLLNEEQMASAITKSRQNKQTLVTTLVQTKLVSARSIAELCYEEYGTPLLDLAEFDVSAIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEEFQFSAGLHAEAILVEEDKLAKALEKVLEEDITGLDLSGMDEAA
LAGIEVTDTDKRQEENAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECKQAEQIPEHELQRLGF
TDEQIAQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIADQAKAQGMRDLRQSGLLKVLQGVTSI
AEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=26867 SHEWANA3_RS02225 WP_011715633.1 476825..478534(-) (pilB) [Shewanella sp. ANA-3]
ATGCCATCCGCTGGCCTTCACCTGGGATTATCCACCCTTTTCATCCGTAAAGGTCTGCTTAATGAAGAGCAGATGGCGAG
TGCCATCACTAAATCCCGCCAAAATAAACAAACCTTAGTCACTACCTTAGTGCAGACTAAGCTAGTGTCGGCTCGCAGTA
TTGCCGAGCTTTGCTATGAGGAATACGGCACCCCGCTGCTGGATTTAGCCGAATTTGATGTTAGTGCCATTCCCGAAGAG
TTTCTGAATAAAAAACTGATTGAAAAGCACCGCTGCTTACCGCTATTTAAGCGCGGTAATCGCCTCTATATCGCCACCTC
TGACCCCACCAATATTGCCGCCCTTGAGGAATTCCAATTTAGTGCAGGCCTACATGCCGAAGCGATTTTGGTGGAAGAAG
ATAAGCTCGCTAAAGCCCTTGAAAAGGTACTAGAAGAAGATATTACTGGCCTTGATTTAAGCGGCATGGATGAAGCAGCA
CTTGCCGGTATCGAAGTTACCGATACCGATAAGCGCCAAGAAGAAAACGCAGGAGAAGCCAGCGATGACGCCCCTATTGT
TATTTATATCAATAAGATATTAACCGATGCCATTCGTAAGGGCGCCTCTGACTTACATTTTGAGCCCTATGAAAAACGCT
ACCGTATCCGTTTTCGTATCGATGGTATTTTGCATGAGGTCTCCGAACCGCCCATTAGTTTAGCAGGGCGACTCTCGGCG
CGCTTAAAGGTAATGTCCAAGCTGGATATTGCCGAGCGCCGCGTCCCACAAGATGGGCGGATTAAAATGAAGCTATCCCG
CACTAAGTCTATCGACTTTCGGGTAAGTACCCTGCCGACCCTGTGGGGCGAGAAGATTGTAATGCGGATATTAGATTCTT
CTTCCGCCCAGCTTGGTATCGAAAAACTCGGTTACGAACCCGATCAAGAAAAGCTTTACCTTGAAATGCTAGCCAAACCT
CAGGGCATGATATTAGTGACAGGCCCAACGGGTTCGGGTAAAACCGTTTCGCTCTACACAGGCTTAAATATCCTCAATAC
CGCCGAGCGTAATATCTCCACTGCCGAAGATCCGGTGGAAATTAACCTTGAGGGGGTAAACCAAGTCCATATTAATTTAA
AAGCGGGTTTAACCTTTGCTTCAGCACTACGTTCGTTTTTGCGGCAAGACCCCGATGTGGTGATGGTCGGGGAAATCCGT
GACCTTGAAACCGCCGAAATTGCCATTAAAGCCGCGCAAACTGGCCACTTAGTATTATCAACCCTGCATACTAACTCGGC
GGCAGAAACGCTCACCCGTTTAATTAATATGGGCGTACCCGGCTATAATATTGCCAGCTCAGTTAATTTAATTATTGCTC
AGCGCCTTGCACGGCGGCTTTGCACTGAATGCAAACAAGCCGAACAGATACCCGAACATGAGCTACAACGCTTAGGCTTT
ACCGATGAGCAAATCGCTCAAGGGTTTACCACCTTTAAACCTGTGGGTTGTGAACATTGTTCTGGTGGCTATAAAGGACG
GGTAGGTATTTACGAAGTGATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGTGGCAATTCATTGCAGATAG
CAGATCAAGCCAAGGCTCAAGGCATGCGCGACCTGCGGCAATCGGGCCTCCTAAAAGTCCTTCAAGGGGTAACTAGCATT
GCAGAAATCAATAGGGTGACAAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0KS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.25

98.594

0.594
  pilB Acinetobacter baumannii D1279779

59.266

95.782

0.568
  pilB Legionella pneumophila strain ERS1305867

56.228

98.77

0.555
  pilB Vibrio cholerae strain A1552

53.514

97.54

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

51.313

100

0.515
  pilB Vibrio campbellii strain DS40M4

51.237

99.473

0.51
  pilF Neisseria gonorrhoeae MS11

49.117

99.473

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.606

98.067

0.388
  pilF Thermus thermophilus HB27

39.037

98.594

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

41.235

88.225

0.364

Multiple sequence alignment