Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AHA_RS18520 Genome accession   NC_008570
Coordinates   4096874..4097983 (+) Length   369 a.a.
NCBI ID   WP_011707390.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila ATCC 7966     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4091874..4102983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AHA_RS18485 (AHA_3659) - 4092492..4092803 (+) 312 WP_042064670.1 hypothetical protein -
  AHA_RS18490 (AHA_3660) - 4092858..4093277 (-) 420 WP_011707384.1 DUF4426 domain-containing protein -
  AHA_RS18495 (AHA_3661) yggU 4093296..4093595 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  AHA_RS18500 (AHA_3662) - 4093595..4094146 (-) 552 WP_011707386.1 YggT family protein -
  AHA_RS18505 (AHA_3663) proC 4094187..4095011 (-) 825 WP_011707387.1 pyrroline-5-carboxylate reductase -
  AHA_RS18510 (AHA_3664) - 4095070..4095771 (-) 702 WP_011707388.1 YggS family pyridoxal phosphate-dependent enzyme -
  AHA_RS18515 (AHA_3665) pilT 4095811..4096845 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  AHA_RS18520 (AHA_3666) pilU 4096874..4097983 (+) 1110 WP_011707390.1 type IVa pilus ATPase TapU Machinery gene
  AHA_RS18525 (AHA_3667) yaaA 4098015..4098788 (+) 774 WP_011707391.1 peroxide stress protein YaaA -
  AHA_RS18530 (AHA_3668) srmB 4098965..4100188 (-) 1224 WP_011707392.1 ATP-dependent RNA helicase SrmB -
  AHA_RS18535 (AHA_3669) - 4100317..4101027 (+) 711 WP_011707393.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  AHA_RS18540 (AHA_3670) brnQ 4101219..4102526 (+) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41077.25 Da        Isoelectric Point: 6.5035

>NTDB_id=26829 AHA_RS18520 WP_011707390.1 4096874..4097983(+) (pilU) [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MNLDVLLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=26829 AHA_RS18520 WP_011707390.1 4096874..4097983(+) (pilU) [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
ATGAATCTGGATGTCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTATCTGCATACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGTTAGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGCTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGTCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment