Detailed information NTG0026569

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LACR_RS09395
• Genome accession: NC_008527
• Coordinates: 1836752..1837333 (-)
• Length: 193 a.a.
• NCBI ID: WP_231097919.1
• Uniprot ID: -
• Organism: Lactococcus cremoris subsp. cremoris SK11
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1831752..1842333

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LACR_RS09370 (LACR_1939)	-	1832096..1832311 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LACR_RS09375 (LACR_1940)	-	1832494..1833270 (-)	777	WP_011676769.1	alpha/beta hydrolase family protein	-
LACR_RS09380	-	1833554..1833859 (-)	306	Protein_1824	ComEC/Rec2 family competence protein	-
LACR_RS09385 (LACR_1943)	-	1833880..1834770 (-)	891	WP_011676771.1	IS982 family transposase	-
LACR_RS14585	comEC	1834847..1835356 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LACR_RS09390	comEC	1835353..1836624 (-)	1272	WP_324187454.1	ComEC/Rec2 family competence protein	Machinery gene
LACR_RS09395 (LACR_1944)	comEA	1836752..1837333 (-)	582	WP_231097919.1	helix-hairpin-helix domain-containing protein	Machinery gene
LACR_RS09400 (LACR_1945)	-	1837464..1838825 (-)	1362	WP_011676773.1	ABC transporter permease	-
LACR_RS09405 (LACR_1946)	-	1838822..1839751 (-)	930	WP_011676774.1	ABC transporter ATP-binding protein	-
LACR_RS09410 (LACR_1947)	-	1839857..1840255 (-)	399	WP_011676775.1	hypothetical protein	-
LACR_RS09415 (LACR_1948)	-	1840372..1840932 (-)	561	WP_011676776.1	GNAT family N-acetyltransferase	-
LACR_RS09420 (LACR_1949)	-	1841105..1842283 (-)	1179	WP_011676777.1	SLC13 family permease	-

Sequence

Protein

• Length: 193 a.a.
• Molecular weight: 21052.51 Da
• Isoelectric Point: 4.8079

>NTDB_id=26569 LACR_RS09395 WP_231097919.1 1836752..1837333(-) (comEA) [Lactococcus cremoris subsp. cremoris SK11]
MEFLYIFTNSPKQAENLSVENLSSSSTKSSVSKFNSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQ
ADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFR
MKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 582 bp

>NTDB_id=26569 LACR_RS09395 WP_231097919.1 1836752..1837333(-) (comEA) [Lactococcus cremoris subsp. cremoris SK11]
GTGGAATTTTTATATATTTTCACCAATTCACCAAAGCAAGCTGAAAATCTGTCAGTAGAAAATTTGAGCTCTAGTTCTAC
CAAAAGCTCTGTCAGTAAATTTAATAGCAGCAGTGAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTG
CAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAA
GCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTAATTTATGTACCAAAACTTGGGGAAAGTTC
AAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATA
TCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGC
ATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAA
AGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (46-101)

• Helix-hairpin-helix motif (HHH_3) (129-190)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	94.792	99.482	0.943
comEA/celA/cilE	Streptococcus mitis SK321	45.213	97.409	0.44
comEA	Latilactobacillus sakei subsp. sakei 23K	42.781	96.891	0.415
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	77.202	0.378
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	77.202	0.368
comEA	Staphylococcus aureus MW2	40	90.674	0.363
comEA	Streptococcus thermophilus LMD-9	43.478	83.42	0.363