Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   C1O61_RS12025 Genome accession   NZ_CP025836
Coordinates   1971328..1972296 (-) Length   322 a.a.
NCBI ID   WP_261370611.1    Uniprot ID   -
Organism   Akkermansia muciniphila strain EB-AMDK-21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1966328..1977296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1O61_RS08370 (C1O61_08755) - 1966789..1967244 (+) 456 WP_102732154.1 nucleoside-diphosphate kinase -
  C1O61_RS08375 (C1O61_08760) - 1967392..1968213 (-) 822 WP_102732153.1 glycosyltransferase family 2 protein -
  C1O61_RS08380 (C1O61_08765) - 1968222..1969166 (-) 945 WP_012420617.1 glycosyltransferase family 2 protein -
  C1O61_RS08385 (C1O61_08770) - 1969301..1969969 (-) 669 WP_094139497.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C1O61_RS08390 (C1O61_08775) - 1969988..1971256 (-) 1269 WP_046435767.1 type II secretion system F family protein -
  C1O61_RS12025 pilB/pilB1 1971328..1972296 (-) 969 WP_261370611.1 GspE/PulE family protein Machinery gene
  C1O61_RS12030 - 1972284..1972985 (-) 702 WP_261370612.1 ATPase, T2SS/T4P/T4SS family -
  C1O61_RS08400 (C1O61_08785) - 1973013..1974653 (-) 1641 WP_022198743.1 ATPase, T2SS/T4P/T4SS family -
  C1O61_RS08405 (C1O61_08790) dusB 1974865..1975869 (-) 1005 WP_022198744.1 tRNA dihydrouridine synthase DusB -
  C1O61_RS08410 (C1O61_08795) - 1976132..1976641 (-) 510 WP_012420623.1 YlbF family regulator -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35493.67 Da        Isoelectric Point: 5.2219

>NTDB_id=264106 C1O61_RS12025 WP_261370611.1 1971328..1972296(-) (pilB/pilB1) [Akkermansia muciniphila strain EB-AMDK-21]
MANMNIAERRIPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILDTVHKPNGIFI
VTGPTGAGKTTTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAIGLDFPMVLRAFLRQDPDRILVGEMRDMATA
QIAIQASLTGHLVLSTLHTNDSAGAITRLVDMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYEL
GDKHFFTGRGCDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYDGNTTIEEVLK
YT

Nucleotide


Download         Length: 969 bp        

>NTDB_id=264106 C1O61_RS12025 WP_261370611.1 1971328..1972296(-) (pilB/pilB1) [Akkermansia muciniphila strain EB-AMDK-21]
ATGGCAAACATGAACATTGCGGAACGCCGCATTCCGCAGGACGGCCGCATCGTCAAGCAGATAGGAAACCGTTCCGTGGA
CATGCGCGTTTCCTCCCTTCCCACTCAATACGGAGAATCCGTGGTGCTCCGCGTTCTGGACCGCTCTTCCGTCAACTTGA
ACATGGACAACCTGGGGCTTCCCGCGCATATCCACGAATATATTCTGGATACGGTCCACAAGCCCAACGGCATTTTCATC
GTTACCGGCCCCACCGGCGCCGGCAAGACAACTACGCTGTATGCCGCCCTGCGTGAAATCAATACCATTGATTCCAAGGT
GCTGACGGCGGAAGACCCTGTTGAATACGATATTGACGGCATCATCCAGATTCCTATCAATGAAGCCATCGGCCTGGATT
TCCCAATGGTTCTCCGCGCCTTCCTGCGACAGGACCCGGACCGTATTCTGGTAGGGGAAATGCGAGACATGGCAACAGCG
CAGATCGCCATCCAGGCATCCCTGACGGGCCACCTAGTTCTCTCCACCCTGCACACGAACGACTCTGCCGGAGCCATTAC
GCGACTGGTGGACATGGGGTGCGAACCTTTCCTGGTAGCGGCCTCCCTGGAAGGGGTGCTTGCACAGCGCCTGGTGCGCA
CCATCTGTCCGGACTGCCGCACCCCGTATGAACCCTCATCCACCATCCTCTCCCAGCTTGGCGTATCTCCCTATGAATTG
GGGGACAAGCACTTTTTCACGGGCCGCGGCTGTGATAAATGCTCCAATTCCGGCTACAGGGGCCGCAAGGGGATTTATGA
GCTTCTGGATATTAACGACACCCTGCGTGACATGATTACGGATCGCGCTCCCTCCGTGGTGCTGAAACAGAAGGCTATTG
AAATGGGCATGTCCACGCTGAGAGAAGACGGGCTGAGAAATATTTATGACGGCAACACTACTATTGAAGAAGTGCTGAAA
TATACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(1-214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

47.493

100

0.5
  pilF Thermus thermophilus HB27

48.758

100

0.488
  pilB Legionella pneumophila strain ERS1305867

45.062

100

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.201

100

0.453
  pilB Vibrio campbellii strain DS40M4

45.171

99.689

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.312

99.379

0.45
  pilB Vibrio cholerae strain A1552

45.141

99.068

0.447
  pilF Neisseria gonorrhoeae MS11

42.945

100

0.435
  ctsE Campylobacter jejuni subsp. jejuni 81-176

43.438

99.379

0.432
  pilB Acinetobacter baylyi ADP1

42.769

100

0.432
  pilB Acinetobacter baumannii D1279779

42.154

100

0.425
  pilB Glaesserella parasuis strain SC1401

40.923

100

0.413
  pilB Haemophilus influenzae 86-028NP

40.312

99.379

0.401
  pilB Haemophilus influenzae Rd KW20

39.062

99.379

0.388

Multiple sequence alignment