Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   C1H71_RS08405 Genome accession   NZ_CP025781
Coordinates   1879138..1879551 (-) Length   137 a.a.
NCBI ID   WP_130106150.1    Uniprot ID   A0A7G3G8Q8
Organism   Iodobacter fluviatilis strain PCH194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1874138..1884551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1H71_RS08395 (C1H71_08470) - 1875381..1878767 (-) 3387 WP_130106148.1 fused MFS/spermidine synthase -
  C1H71_RS08400 - 1878764..1879033 (-) 270 WP_130106149.1 hypothetical protein -
  C1H71_RS08405 (C1H71_08480) pilA2 1879138..1879551 (-) 414 WP_130106150.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  C1H71_RS08415 (C1H71_08490) - 1879990..1880592 (-) 603 WP_130106151.1 nucleoside triphosphate pyrophosphatase -
  C1H71_RS08420 (C1H71_08495) rlmH 1880595..1881065 (-) 471 WP_130106152.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  C1H71_RS08425 (C1H71_08500) - 1881062..1882609 (-) 1548 WP_130106153.1 EAL domain-containing protein -
  C1H71_RS08430 (C1H71_08505) - 1882679..1883353 (+) 675 WP_130106154.1 TIGR01621 family pseudouridine synthase -
  C1H71_RS08435 (C1H71_08510) - 1883390..1884157 (-) 768 WP_130106155.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14065.17 Da        Isoelectric Point: 8.0945

>NTDB_id=263744 C1H71_RS08405 WP_130106150.1 1879138..1879551(-) (pilA2) [Iodobacter fluviatilis strain PCH194]
MKNIQKGFTLIELMIVVAIIGILAAVAIPSYQDYTKKAKYSEVLSISDSYKQAVALCQSDLNTFAGCNAGTQGIPATPTV
ATTGLATGGTVTNGVINFSGTAAAGGYTFILTPSLNAEGTAITWAQSGNCLAAKYCK

Nucleotide


Download         Length: 414 bp        

>NTDB_id=263744 C1H71_RS08405 WP_130106150.1 1879138..1879551(-) (pilA2) [Iodobacter fluviatilis strain PCH194]
ATGAAAAACATACAAAAGGGTTTTACCCTGATCGAACTGATGATTGTTGTGGCTATTATTGGTATCTTAGCTGCTGTAGC
TATTCCGTCTTATCAGGATTACACCAAGAAAGCGAAGTACTCTGAAGTATTAAGTATTTCTGATTCTTACAAACAAGCTG
TTGCATTATGCCAATCTGATTTGAATACCTTTGCCGGATGTAATGCAGGCACTCAGGGTATTCCTGCAACTCCCACTGTT
GCTACTACTGGTTTGGCTACTGGTGGTACTGTTACTAATGGCGTGATTAATTTTTCTGGAACAGCCGCAGCAGGTGGATA
TACTTTTATTTTAACTCCTTCTTTGAATGCTGAAGGTACGGCAATTACTTGGGCACAATCAGGTAATTGTTTAGCTGCTA
AGTACTGCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G3G8Q8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.632

97.08

0.511
  pilA2 Legionella pneumophila strain ERS1305867

51.145

95.62

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

45.522

97.81

0.445
  comP Acinetobacter baylyi ADP1

44.275

95.62

0.423
  pilA Haemophilus influenzae 86-028NP

47.154

89.781

0.423
  pilA Haemophilus influenzae Rd KW20

44.355

90.511

0.401
  pilA Glaesserella parasuis strain SC1401

41.538

94.891

0.394
  pilA/pilA1 Eikenella corrodens VA1

39.394

96.35

0.38

Multiple sequence alignment