Detailed information NTG0262103

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLG50_RS08995
• Genome accession: NZ_CP025500
• Coordinates: 1846993..1847640 (-)
• Length: 215 a.a.
• NCBI ID: WP_103055061.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain G50
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1841993..1852640

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLG50_RS08970 (LLG50_08970)	atpF	1842063..1842569 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLG50_RS08975 (LLG50_08975)	atpB	1842584..1843297 (-)	714	WP_103055058.1	F0F1 ATP synthase subunit A	-
LLG50_RS08980 (LLG50_08980)	-	1843342..1843557 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLG50_RS08985 (LLG50_08985)	-	1843742..1844518 (-)	777	WP_103055059.1	alpha/beta hydrolase	-
LLG50_RS08990 (LLG50_08990)	comEC	1844802..1847012 (-)	2211	WP_103055060.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLG50_RS08995 (LLG50_08995)	comEA	1846993..1847640 (-)	648	WP_103055061.1	ComEA family DNA-binding protein	Machinery gene
LLG50_RS09000 (LLG50_09000)	-	1847700..1849061 (-)	1362	WP_029344292.1	ABC transporter permease	-
LLG50_RS09005 (LLG50_09005)	-	1849058..1849990 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLG50_RS09010 (LLG50_09010)	-	1850095..1850493 (-)	399	WP_103055062.1	hypothetical protein	-
LLG50_RS09015 (LLG50_09015)	-	1850597..1851160 (-)	564	WP_103055063.1	GNAT family N-acetyltransferase	-
LLG50_RS09020 (LLG50_09020)	-	1851333..1852511 (-)	1179	WP_103055064.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23563.73 Da
• Isoelectric Point: 4.9882

>NTDB_id=262103 LLG50_RS08995 WP_103055061.1 1846993..1847640(-) (comEA) [Lactococcus lactis subsp. lactis strain G50]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENASTENTDSSANSVNSQVSTTTAKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=262103 LLG50_RS08995 WP_103055061.1 1846993..1847640(-) (comEA) [Lactococcus lactis subsp. lactis strain G50]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATGCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCAATAGTCAAGTCTCAACAACCACTGCAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (152-212)

• SLBB domain (SLBB) (70-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	74.771	100	0.758
comEA/celA/cilE	Streptococcus mitis SK321	43.519	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367
comEA	Staphylococcus aureus N315	36.62	99.07	0.363