Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CYK59_RS06850 Genome accession   NZ_CP025476
Coordinates   1308960..1309835 (-) Length   291 a.a.
NCBI ID   WP_126133420.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain IRG2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1303960..1314835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYK59_RS06820 (CYK59_06845) - 1305061..1306071 (-) 1011 WP_054644024.1 class I SAM-dependent methyltransferase -
  CYK59_RS06825 (CYK59_06850) - 1306196..1306519 (-) 324 WP_039099486.1 hypothetical protein -
  CYK59_RS06830 (CYK59_06855) - 1306497..1306877 (-) 381 WP_004270914.1 ComGF family competence protein -
  CYK59_RS10660 - 1306899..1306982 (-) 84 Protein_1313 type II secretion system protein -
  CYK59_RS10280 - 1306948..1307253 (-) 306 WP_039099485.1 hypothetical protein -
  CYK59_RS06835 (CYK59_06860) - 1307240..1307683 (-) 444 WP_039099484.1 type II secretion system protein -
  CYK59_RS06840 (CYK59_06865) comGC 1307664..1307972 (-) 309 WP_004270901.1 competence type IV pilus major pilin ComGC Machinery gene
  CYK59_RS06845 (CYK59_06870) comGB 1307969..1308970 (-) 1002 WP_076786758.1 type II secretion system F family protein Machinery gene
  CYK59_RS06850 (CYK59_06875) comGA 1308960..1309835 (-) 876 WP_126133420.1 competence type IV pilus ATPase ComGA Machinery gene
  CYK59_RS06855 (CYK59_06880) - 1309939..1310670 (-) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CYK59_RS06860 (CYK59_06885) - 1310760..1311260 (-) 501 WP_004270893.1 VanZ family protein -
  CYK59_RS06865 (CYK59_06890) - 1311363..1311740 (+) 378 WP_039099519.1 hypothetical protein -
  CYK59_RS06875 (CYK59_06900) - 1311921..1312256 (-) 336 WP_076786752.1 hypothetical protein -
  CYK59_RS06880 (CYK59_06905) - 1312451..1312690 (+) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  CYK59_RS06885 (CYK59_06910) tnpA 1312817..1313128 (-) 312 Protein_1324 IS200/IS605 family transposase -
  CYK59_RS06895 (CYK59_06920) - 1314315..1314461 (-) 147 Protein_1326 transposase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32061.88 Da        Isoelectric Point: 5.3292

>NTDB_id=261910 CYK59_RS06850 WP_126133420.1 1308960..1309835(-) (comGA) [Latilactobacillus curvatus strain IRG2]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQIMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVEGQNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=261910 CYK59_RS06850 WP_126133420.1 1308960..1309835(-) (comGA) [Latilactobacillus curvatus strain IRG2]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAATCATGCAAGATAAAATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCGTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTCTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCAGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGAAGGCCAAAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368

Multiple sequence alignment