Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABO_RS03170 Genome accession   NC_008260
Coordinates   674017..675723 (-) Length   568 a.a.
NCBI ID   WP_011587899.1    Uniprot ID   Q0VRY6
Organism   Alcanivorax borkumensis SK2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 669017..680723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABO_RS03145 (ABO_0609) - 669953..670891 (+) 939 WP_011587894.1 Nudix family hydrolase -
  ABO_RS03150 (ABO_0610) - 671005..671205 (-) 201 WP_011587895.1 DNA gyrase inhibitor YacG -
  ABO_RS03155 (ABO_0611) coaE 671227..671847 (-) 621 WP_011587896.1 dephospho-CoA kinase -
  ABO_RS03160 (ABO_0612) pilD 671910..672785 (-) 876 WP_011587897.1 prepilin peptidase Machinery gene
  ABO_RS03165 (ABO_0613) pilC 672788..674014 (-) 1227 WP_035460653.1 type II secretion system F family protein Machinery gene
  ABO_RS03170 (ABO_0614) pilB 674017..675723 (-) 1707 WP_011587899.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABO_RS14630 (ABO_0615) comP 676258..676704 (+) 447 WP_011587900.1 pilin Machinery gene
  ABO_RS03180 (ABO_0616) - 676720..678087 (+) 1368 WP_041704871.1 O-antigen ligase family protein -
  ABO_RS03185 (ABO_0617) nadC 678143..678985 (-) 843 WP_011587902.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABO_RS03190 (ABO_0618) - 679101..680438 (-) 1338 WP_011587903.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62722.46 Da        Isoelectric Point: 5.0177

>NTDB_id=26147 ABO_RS03170 WP_011587899.1 674017..675723(-) (pilB) [Alcanivorax borkumensis SK2]
MTSQTTSFSGLPHRLIQQGLLDAESIQLALQQARQEKTSLIKQIVASGKLTARQVASAVAEEFGDPIFDLNSFQRDSLVK
DLVDPKLINKHNVLPLYRRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEEDKLSQWIEALESETEDDAFSDLDAGL
ENLDISSNEETEDETQSGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGILQTVSKPPINIASKLAARL
KVMSRLDISERRVPQDGRIKLKISKTRAIDFRVNTCPTLFGEKIVLRILDPESAKMGIEALGYENDQKQMYLDALHKPQG
MILVTGPTGSGKTVSLYTGVNILNTPELNISTAEDPVEINLEGVNQVNVNPKQGMNFSSALRAFLRQDPDVILVGEIRDL
ETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVLLIIAQRLARRLCENCKTPVDIPKQSLLEMGFTE
DDIANEFTVYGPVGCDKCKNGYKGRVGIYEVVKITDGIARIIMEEGNSIEIADQCRKEGFNNLHRSGLVKAMQGVTSLEE
INRVTSGH

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=26147 ABO_RS03170 WP_011587899.1 674017..675723(-) (pilB) [Alcanivorax borkumensis SK2]
ATGACTAGCCAAACCACCTCATTTAGCGGCTTACCGCATCGATTAATACAGCAGGGCCTGCTAGACGCAGAGAGCATACA
GCTAGCCCTGCAACAGGCTCGCCAAGAAAAAACCAGCCTTATTAAACAGATCGTTGCTAGTGGGAAACTTACGGCTAGGC
AGGTAGCAAGCGCCGTTGCGGAAGAATTTGGCGACCCTATTTTTGACCTTAACAGCTTCCAGCGCGATAGCTTAGTAAAG
GACCTTGTCGACCCTAAGCTGATCAATAAACACAACGTACTCCCCCTTTACCGGCGCGGAAGCCGCCTCTTTGTTGCGGT
ATCCGATCCTACTAATTTACCTGCGCTAGAAGAAATCCGTTTTAATACTGGCCTTAATGTAGAAACGGTGATTGTTGAGG
AAGACAAGCTAAGCCAATGGATCGAAGCGTTGGAGTCCGAGACCGAAGATGATGCCTTTAGCGACCTGGATGCCGGACTA
GAAAACCTGGACATTAGTTCGAATGAAGAAACAGAAGATGAAACTCAATCTGGTGCCGATGATGCACCAATTGTCCGCTT
CGTGAATAAACTTCTTCTTGATGCCATTAAGGGCGGAGCATCAGATTTGCATTTCGAGCCCTATGAGAAAACCTATCGGG
TGCGCTTCCGTCAAGATGGCATCCTGCAAACCGTATCGAAACCACCTATCAATATCGCCAGCAAACTTGCTGCGCGCCTA
AAGGTCATGTCTCGGCTGGATATTTCAGAACGTCGAGTCCCTCAGGATGGCCGGATAAAGTTGAAAATTTCCAAGACACG
GGCCATCGATTTCCGGGTGAACACCTGCCCCACCTTATTTGGTGAAAAAATTGTATTGCGTATTCTGGACCCGGAATCCG
CAAAAATGGGCATCGAGGCTTTGGGTTATGAAAATGATCAGAAACAGATGTACCTGGATGCCCTGCATAAACCACAAGGC
ATGATACTAGTCACTGGCCCCACCGGCTCAGGCAAGACCGTTTCACTATACACCGGAGTAAACATTCTCAATACGCCAGA
ACTCAATATTTCCACCGCCGAAGACCCGGTTGAAATCAACTTGGAGGGGGTTAACCAGGTTAATGTGAACCCGAAACAAG
GGATGAATTTCTCATCAGCACTACGTGCCTTCCTACGCCAGGATCCCGACGTCATTCTGGTGGGAGAAATTCGTGACTTA
GAAACAGCAGAAATAGCTGTGAAAGCGGCCCAGACAGGTCACCTGGTACTTTCCACCCTCCACACTAACAGCGCACCGGA
AACTCTAACTCGTCTTCGCAACATGGGCGTTCCCTCCTTCAACATTGCCACCTCGGTATTATTAATTATTGCCCAACGTT
TAGCCCGGCGCCTGTGTGAAAACTGCAAAACTCCAGTGGATATTCCTAAACAATCCTTGCTTGAGATGGGTTTCACGGAA
GACGATATCGCCAACGAGTTTACTGTGTACGGACCGGTAGGGTGCGACAAATGCAAGAACGGCTACAAAGGGCGAGTGGG
GATTTATGAAGTCGTTAAAATCACAGACGGCATCGCGCGCATTATTATGGAAGAGGGTAACTCTATCGAAATTGCCGACC
AGTGCCGCAAGGAGGGCTTTAATAACCTTCACCGCTCCGGATTGGTAAAAGCAATGCAAGGGGTTACCAGCCTAGAAGAA
ATTAACCGTGTCACAAGCGGACATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0VRY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.632

100

0.629
  pilB Acinetobacter baylyi ADP1

60.839

100

0.613
  pilB Legionella pneumophila strain ERS1305867

55.07

100

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

50.176

100

0.502
  pilB Vibrio campbellii strain DS40M4

50.268

98.415

0.495
  pilB Vibrio cholerae strain A1552

49.821

98.592

0.491
  pilF Neisseria gonorrhoeae MS11

48.566

98.239

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

37.398

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

96.831

0.373
  pilF Thermus thermophilus HB27

37.658

97.711

0.368

Multiple sequence alignment