Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CW735_RS14555 Genome accession   NZ_CP025115
Coordinates   3272292..3273503 (+) Length   403 a.a.
NCBI ID   WP_100972785.1    Uniprot ID   -
Organism   Alteromonas sp. MB-3u-76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3267292..3278503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CW735_RS14530 (CW735_14530) ampE 3268123..3269022 (-) 900 WP_100972781.1 beta-lactamase regulator AmpE -
  CW735_RS14535 (CW735_14535) ampD 3269132..3269671 (-) 540 WP_100972782.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CW735_RS14540 (CW735_14540) - 3269935..3270453 (+) 519 WP_100972783.1 TIGR02281 family clan AA aspartic protease -
  CW735_RS14545 (CW735_14545) nadC 3270487..3271341 (+) 855 WP_198033618.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CW735_RS14550 (CW735_14550) - 3271788..3272210 (+) 423 WP_100972784.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CW735_RS14555 (CW735_14555) pilC 3272292..3273503 (+) 1212 WP_100972785.1 type II secretion system F family protein Machinery gene
  CW735_RS14560 (CW735_14560) pilD 3273666..3274574 (+) 909 WP_100972786.1 A24 family peptidase Machinery gene
  CW735_RS14565 (CW735_14565) coaE 3274575..3275180 (+) 606 WP_198520976.1 dephospho-CoA kinase -
  CW735_RS14570 (CW735_14570) zapD 3275579..3276331 (+) 753 WP_071980369.1 cell division protein ZapD -
  CW735_RS14575 (CW735_14575) yacG 3276581..3276808 (+) 228 WP_071980370.1 DNA gyrase inhibitor YacG -
  CW735_RS14580 (CW735_14580) - 3276830..3277726 (+) 897 WP_071980371.1 aspartoacylase -
  CW735_RS14585 (CW735_14585) mutT 3277916..3278308 (-) 393 WP_071980372.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43706.36 Da        Isoelectric Point: 9.4405

>NTDB_id=258406 CW735_RS14555 WP_100972785.1 3272292..3273503(+) (pilC) [Alteromonas sp. MB-3u-76]
MARAPAVFTWQGKDRRGQSRKGEISAVSLSEAKNLLRRQGISANKVKKLSKPLFGSGAAKITPADISVTSRQIATMLSAG
VTLIQSLEMIAQGHEKVSMRKLLGEITEEVKAGNPLSSALRKHPNYFDDLYCDLVYTGEQSGALELIYDRIATYKEKAEA
LKSKIKKAMFYPIAVVVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLGISRFVQDYGIFIAIGVGVAGFLFGRA
YKKSQNLRDRCDRLVLKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYREAILYMRKEVAGGIPM
HVAMRATNVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIIAMYLPIFDMG
NVV

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=258406 CW735_RS14555 WP_100972785.1 3272292..3273503(+) (pilC) [Alteromonas sp. MB-3u-76]
ATGGCAAGAGCACCAGCAGTTTTTACATGGCAGGGCAAAGACAGAAGGGGCCAGTCGCGGAAAGGTGAAATTTCCGCAGT
TTCACTTTCAGAAGCCAAGAATCTTCTCCGACGACAGGGTATTTCCGCTAACAAGGTCAAGAAGTTAAGCAAACCTTTAT
TCGGAAGCGGCGCAGCAAAAATAACACCAGCAGACATCTCTGTTACCTCTCGTCAAATAGCAACCATGCTAAGCGCTGGT
GTTACGCTTATACAGTCTCTTGAAATGATTGCCCAAGGCCATGAAAAAGTTAGCATGCGCAAGTTGCTTGGCGAGATTAC
CGAGGAAGTAAAGGCCGGAAATCCTCTATCTTCAGCCCTTAGAAAACATCCCAACTATTTCGATGATTTGTATTGCGACC
TGGTTTATACCGGTGAGCAGTCAGGTGCCCTAGAGCTTATTTACGACCGAATAGCCACATACAAGGAAAAAGCCGAAGCC
TTAAAATCTAAAATCAAAAAGGCGATGTTTTACCCTATTGCGGTAGTCGTAGTTGCTTTTATTGTTACTACTATTCTTCT
TATTTTTGTAGTGCCTCAATTTGAAGAGATTTTTAGCAGTTTCGGTGCTGAGTTGCCTGCGTTTACGCAATTTGTATTGG
GTATTTCTCGCTTTGTACAAGATTACGGTATTTTTATCGCGATTGGTGTGGGTGTTGCTGGCTTTTTATTTGGCCGTGCT
TATAAAAAGAGCCAAAACTTACGTGATCGTTGCGATAGATTGGTATTGAAAATTCCTGTGATTGGGGAAATACTAAAAAA
GGCCAGCATCGCACGTTTTACCCGCACCCTTGCAACAACATTCGCCGCTGGTGTACCTCTGATAGGCGCACTTGAATCTG
CCGCCGGCGCATCTGGCAATGCCGTATATAGGGAAGCGATATTATATATGCGTAAAGAAGTGGCTGGCGGTATTCCCATG
CATGTGGCTATGCGTGCGACTAACGTATTCCCCGACATGGTGACGCAGATGATTGCTATTGGCGAGGAGTCTGGTGCTGT
AGATGAAATGCTAAGTAAGATTGCCACAATATACGAAGCGGAAGTAGATGACATGGTAGACGGTTTAACCAGCTTACTAG
AGCCTATGATTATGGCTGTACTAGGTGTGGTAATCGGCGGCCTAATTATTGCCATGTACCTACCTATATTTGATATGGGT
AACGTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.045

98.759

0.583
  pilC Acinetobacter baylyi ADP1

53.75

99.256

0.533
  pilC Acinetobacter baumannii D1279779

52.897

98.511

0.521
  pilC Legionella pneumophila strain ERS1305867

52.01

98.759

0.514
  pilC Vibrio cholerae strain A1552

46.212

98.263

0.454
  pilG Neisseria gonorrhoeae MS11

44.226

100

0.447
  pilC Vibrio campbellii strain DS40M4

44.081

98.511

0.434
  pilG Neisseria meningitidis 44/76-A

44.909

95.037

0.427
  pilC Thermus thermophilus HB27

37.562

99.752

0.375

Multiple sequence alignment