Detailed information NTG0258407

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CW735_RS14560
• Genome accession: NZ_CP025115
• Coordinates: 3273666..3274574 (+)
• Length: 302 a.a.
• NCBI ID: WP_100972786.1
• Uniprot ID: -
• Organism: Alteromonas sp. MB-3u-76
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3268666..3279574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CW735_RS14535 (CW735_14535)	ampD	3269132..3269671 (-)	540	WP_100972782.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
CW735_RS14540 (CW735_14540)	-	3269935..3270453 (+)	519	WP_100972783.1	TIGR02281 family clan AA aspartic protease	-
CW735_RS14545 (CW735_14545)	nadC	3270487..3271341 (+)	855	WP_198033618.1	carboxylating nicotinate-nucleotide diphosphorylase	-
CW735_RS14550 (CW735_14550)	-	3271788..3272210 (+)	423	WP_100972784.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CW735_RS14555 (CW735_14555)	pilC	3272292..3273503 (+)	1212	WP_100972785.1	type II secretion system F family protein	Machinery gene
CW735_RS14560 (CW735_14560)	pilD	3273666..3274574 (+)	909	WP_100972786.1	A24 family peptidase	Machinery gene
CW735_RS14565 (CW735_14565)	coaE	3274575..3275180 (+)	606	WP_198520976.1	dephospho-CoA kinase	-
CW735_RS14570 (CW735_14570)	zapD	3275579..3276331 (+)	753	WP_071980369.1	cell division protein ZapD	-
CW735_RS14575 (CW735_14575)	yacG	3276581..3276808 (+)	228	WP_071980370.1	DNA gyrase inhibitor YacG	-
CW735_RS14580 (CW735_14580)	-	3276830..3277726 (+)	897	WP_071980371.1	aspartoacylase	-
CW735_RS14585 (CW735_14585)	mutT	3277916..3278308 (-)	393	WP_071980372.1	8-oxo-dGTP diphosphatase MutT	-

Sequence

Protein

• Length: 302 a.a.
• Molecular weight: 33255.02 Da
• Isoelectric Point: 5.2493

>NTDB_id=258407 CW735_RS14560 WP_100972786.1 3273666..3274574(+) (pilD) [Alteromonas sp. MB-3u-76]
MDAMITLSQLHPEFFYLLVFSVSLMVGSFLNVVIYRLPIMMERSWQEEYESYFADTDEGTNSTHGSKSDSESSSAPFNLV
KPDSTCPKCGHTIRAWENIPVISYVLLKGKCASCKTGISPRYPLVELFTAIACTFTAFHFGPTPQALWAVLFTYILIALI
FIDIDKMLLPDQLTLPLLWLGLLLSTNSIFVSTTDAIIGAAAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAALGAYTGW
QGLPVIILLSSFVGAVVGIAIMVAQNKGKSLAIPFGPYLAVAGWLTFLYKDTIISNYLELVL

Nucleotide

• Length: 909 bp

>NTDB_id=258407 CW735_RS14560 WP_100972786.1 3273666..3274574(+) (pilD) [Alteromonas sp. MB-3u-76]
ATGGATGCAATGATCACCCTTAGCCAACTTCACCCAGAGTTCTTCTATCTACTCGTGTTTTCAGTCAGCTTAATGGTTGG
CAGCTTTTTGAATGTGGTTATTTATCGCCTACCCATTATGATGGAGCGTAGTTGGCAAGAGGAATACGAAAGTTATTTTG
CCGACACTGATGAAGGGACCAATTCTACACATGGTTCTAAGAGTGACTCCGAGAGCAGTTCAGCCCCTTTCAACCTCGTT
AAACCAGACAGCACCTGCCCCAAATGTGGCCACACTATTCGCGCTTGGGAAAATATTCCGGTAATAAGCTATGTGCTATT
AAAAGGTAAATGCGCCAGTTGTAAAACAGGTATATCTCCTCGATATCCATTGGTAGAACTGTTTACAGCCATTGCATGTA
CCTTTACCGCTTTTCATTTTGGTCCTACTCCTCAGGCGCTTTGGGCAGTTTTATTTACCTACATACTTATAGCGCTTATT
TTTATCGACATAGATAAAATGCTGCTGCCCGACCAACTCACCCTGCCCCTGCTATGGCTTGGCTTACTGCTAAGTACTAA
TAGCATTTTTGTGAGCACCACCGACGCTATTATAGGCGCTGCTGCTGGGTATCTTAGTTTATGGTCTGTGTATTGGCTGT
TTAAGCTTGTTACTGGCAAAGAAGGAATGGGCTATGGCGATTTTAAATTACTTGCAGCGCTTGGTGCCTACACGGGCTGG
CAAGGTTTGCCAGTAATAATATTATTATCATCTTTCGTTGGTGCGGTGGTAGGTATCGCCATAATGGTGGCGCAAAATAA
AGGCAAGTCACTGGCTATCCCCTTTGGCCCTTATTTAGCAGTGGCAGGTTGGCTTACCTTTTTGTACAAAGATACTATTA
TTTCTAACTATCTCGAGCTTGTATTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (23-140)

• Type IV leader peptidase family (Peptidase_A24) (151-259)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.041	97.351	0.507
pilD	Vibrio campbellii strain DS40M4	51.864	97.682	0.507
pilD	Neisseria gonorrhoeae MS11	48.966	96.026	0.47
pilD	Acinetobacter baumannii D1279779	49.296	94.04	0.464
pilD	Acinetobacter nosocomialis M2	48.944	94.04	0.46