Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CWI20_RS25430 Genome accession   NZ_CP025056
Coordinates   5444992..5446212 (+) Length   406 a.a.
NCBI ID   WP_003094649.1    Uniprot ID   A0AAQ3LMH2
Organism   Pseudomonas aeruginosa strain PB367     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5439992..5451212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI20_RS25410 (CWI20_25985) nadC 5441542..5442390 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI20_RS25420 (CWI20_25995) - 5442594..5443058 (-) 465 WP_031630343.1 pilin -
  CWI20_RS25425 (CWI20_26000) pilB 5443288..5444988 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI20_RS25430 (CWI20_26005) pilC 5444992..5446212 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  CWI20_RS25435 (CWI20_26010) pilD 5446216..5447088 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI20_RS25440 (CWI20_26015) coaE 5447085..5447696 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CWI20_RS25445 (CWI20_26020) yacG 5447693..5447893 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CWI20_RS25450 (CWI20_26025) - 5447930..5448139 (-) 210 WP_003094660.1 hypothetical protein -
  CWI20_RS25455 (CWI20_26030) - 5448245..5448934 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  CWI20_RS25460 (CWI20_26035) - 5448931..5449401 (-) 471 WP_003094664.1 hypothetical protein -
  CWI20_RS25465 (CWI20_26040) - 5449398..5449823 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  CWI20_RS25470 (CWI20_26045) - 5449956..5450585 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  CWI20_RS25475 (CWI20_26050) - 5450582..5451031 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44058.92 Da        Isoelectric Point: 9.8103

>NTDB_id=257879 CWI20_RS25430 WP_003094649.1 5444992..5446212(+) (pilC) [Pseudomonas aeruginosa strain PB367]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=257879 CWI20_RS25430 WP_003094649.1 5444992..5446212(+) (pilC) [Pseudomonas aeruginosa strain PB367]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCAGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCTTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

99.754

0.786
  pilC Acinetobacter baylyi ADP1

61.029

100

0.613
  pilC Acinetobacter baumannii D1279779

60.396

99.507

0.601
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria gonorrhoeae MS11

46.04

99.507

0.458
  pilG Neisseria meningitidis 44/76-A

45.522

99.015

0.451
  pilC Vibrio cholerae strain A1552

41.626

100

0.416
  pilC Vibrio campbellii strain DS40M4

40.796

99.015

0.404
  pilC Thermus thermophilus HB27

38.155

98.768

0.377

Multiple sequence alignment