Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CWI20_RS25425 Genome accession   NZ_CP025056
Coordinates   5443288..5444988 (+) Length   566 a.a.
NCBI ID   WP_003094647.1    Uniprot ID   A0AAQ3LJE5
Organism   Pseudomonas aeruginosa strain PB367     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5438288..5449988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI20_RS25400 - 5438608..5438850 (-) 243 Protein_5043 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CWI20_RS25405 (CWI20_25980) - 5439001..5441256 (-) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  CWI20_RS25410 (CWI20_25985) nadC 5441542..5442390 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI20_RS25420 (CWI20_25995) - 5442594..5443058 (-) 465 WP_031630343.1 pilin -
  CWI20_RS25425 (CWI20_26000) pilB 5443288..5444988 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI20_RS25430 (CWI20_26005) pilC 5444992..5446212 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  CWI20_RS25435 (CWI20_26010) pilD 5446216..5447088 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI20_RS25440 (CWI20_26015) coaE 5447085..5447696 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CWI20_RS25445 (CWI20_26020) yacG 5447693..5447893 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CWI20_RS25450 (CWI20_26025) - 5447930..5448139 (-) 210 WP_003094660.1 hypothetical protein -
  CWI20_RS25455 (CWI20_26030) - 5448245..5448934 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  CWI20_RS25460 (CWI20_26035) - 5448931..5449401 (-) 471 WP_003094664.1 hypothetical protein -
  CWI20_RS25465 (CWI20_26040) - 5449398..5449823 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62329.20 Da        Isoelectric Point: 5.5814

>NTDB_id=257878 CWI20_RS25425 WP_003094647.1 5443288..5444988(+) (pilB) [Pseudomonas aeruginosa strain PB367]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=257878 CWI20_RS25425 WP_003094647.1 5443288..5444988(+) (pilB) [Pseudomonas aeruginosa strain PB367]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCTCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGGGC
ATGATTCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.551
  pilB Vibrio cholerae strain A1552

51.444

97.88

0.504
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.622

94.346

0.496
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.091

97.173

0.38

Multiple sequence alignment