Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BPRO_RS04145 Genome accession   NC_007948
Coordinates   865142..866359 (+) Length   405 a.a.
NCBI ID   WP_011481800.1    Uniprot ID   Q12F94
Organism   Polaromonas sp. JS666     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860142..871359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPRO_RS04120 (Bpro_0838) rpmA 861329..861586 (-) 258 WP_011481796.1 50S ribosomal protein L27 -
  BPRO_RS04125 (Bpro_0839) rplU 861625..861936 (-) 312 WP_011481797.1 50S ribosomal protein L21 -
  BPRO_RS04130 (Bpro_0840) - 862114..863097 (+) 984 WP_011481798.1 polyprenyl synthetase family protein -
  BPRO_RS04140 (Bpro_0841) pilB 863324..865060 (+) 1737 WP_011481799.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BPRO_RS04145 (Bpro_0842) pilC 865142..866359 (+) 1218 WP_011481800.1 type II secretion system F family protein Machinery gene
  BPRO_RS04150 (Bpro_0843) - 866359..867216 (+) 858 WP_011481801.1 prepilin peptidase -
  BPRO_RS04155 (Bpro_0844) coaE 867221..867844 (+) 624 WP_011481802.1 dephospho-CoA kinase -
  BPRO_RS04160 (Bpro_0845) zapD 867888..868643 (+) 756 WP_011481803.1 cell division protein ZapD -
  BPRO_RS04165 (Bpro_0846) - 868685..868891 (+) 207 WP_011481804.1 DNA gyrase inhibitor YacG -
  BPRO_RS04170 (Bpro_0847) - 868986..869261 (+) 276 WP_011481805.1 hypothetical protein -
  BPRO_RS04175 (Bpro_0848) grxD 869314..869643 (-) 330 WP_011481806.1 Grx4 family monothiol glutaredoxin -
  BPRO_RS04180 (Bpro_0849) prmC 869833..870696 (-) 864 WP_011481807.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44074.84 Da        Isoelectric Point: 9.3396

>NTDB_id=25676 BPRO_RS04145 WP_011481800.1 865142..866359(+) (pilC) [Polaromonas sp. JS666]
MATAVSKGVKEFVFEWEGKDRGGKPVRGEIRASGENQVKSSLRRQGVLPTKIKKRSMRSGKAIKPKDIAIFTRQLATMMK
AGVPLLQSFDIVGRGNPNGSVTKLLNDVRTDVETGTSLSAAFRKYPLYFNALYCNLVEAGEAAGILESLLDRLAVYMEKT
EAIKSKIKSALMYPISVVVVAFVVVAVIMIFVIPAFKEVFTSFGADLPAPTLFVIAVSEFFVAYWWLIFGGIGGSLYFFM
QAWKRNEKMQKFMDRLLLKVPVFGPLIEKSCIARWTRTLSTMFAAGVPLVEALDSVGGASGNSLYADATEKIQQEVSTGT
SLTAAMGNANLFPTMVLQMCAIGEESGSVDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=25676 BPRO_RS04145 WP_011481800.1 865142..866359(+) (pilC) [Polaromonas sp. JS666]
ATGGCAACTGCAGTATCCAAAGGCGTCAAGGAATTTGTTTTCGAGTGGGAGGGCAAGGACCGGGGGGGTAAACCGGTCCG
TGGCGAGATCCGTGCCTCTGGCGAGAACCAGGTCAAGTCTTCCTTGCGCCGGCAGGGTGTGTTGCCCACCAAAATCAAGA
AGCGCAGCATGCGCTCGGGCAAGGCCATCAAGCCCAAGGATATTGCCATTTTCACCCGCCAGCTCGCCACGATGATGAAG
GCGGGTGTGCCCCTGTTGCAGTCATTTGACATCGTCGGTCGCGGCAACCCCAACGGCAGCGTGACCAAACTGCTCAATGA
TGTACGCACCGATGTGGAAACCGGGACTTCGCTGAGCGCTGCGTTTCGCAAATATCCGCTGTATTTCAATGCGCTGTATT
GCAACCTTGTCGAGGCCGGTGAAGCCGCCGGTATTCTTGAGTCCCTGCTGGATCGCCTGGCGGTCTACATGGAAAAGACC
GAGGCCATCAAGTCAAAAATCAAGTCCGCCCTGATGTACCCGATCTCGGTGGTTGTGGTGGCCTTTGTGGTGGTGGCGGT
GATCATGATTTTTGTGATCCCGGCCTTCAAGGAGGTGTTTACCTCGTTTGGTGCCGATTTGCCGGCACCCACGCTGTTTG
TGATAGCCGTCAGCGAGTTCTTCGTGGCTTACTGGTGGCTGATTTTTGGAGGCATTGGTGGCTCCCTGTATTTTTTCATG
CAGGCGTGGAAGCGCAACGAAAAGATGCAGAAGTTCATGGACCGGCTCCTGCTCAAGGTGCCCGTGTTTGGGCCCCTCAT
TGAAAAGTCATGTATTGCCCGCTGGACCCGTACGCTCTCCACCATGTTTGCGGCCGGCGTGCCGCTCGTGGAGGCGCTGG
ATTCCGTGGGAGGCGCATCCGGCAATTCGCTCTATGCTGATGCCACGGAAAAAATCCAGCAGGAAGTTTCCACCGGCACC
AGCCTGACGGCGGCGATGGGCAATGCCAACCTGTTTCCCACCATGGTGCTGCAGATGTGCGCCATCGGTGAGGAGTCCGG
CTCGGTCGACCACATGCTGGGCAAGGCCGCCGATTTCTATGAGGCGGAAGTCGATGAAATGGTGGCCGGCCTGTCCAGCC
TGATGGAGCCTATCATCATCGTGTTTCTGGGCACGTTGATTGGCGGTATCGTGGTTTCCATGTACCTGCCCATTTTCAAG
CTGGGTCAAGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q12F94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.478

99.259

0.541
  pilC Legionella pneumophila strain ERS1305867

51.508

98.272

0.506
  pilG Neisseria gonorrhoeae MS11

51.25

98.765

0.506
  pilG Neisseria meningitidis 44/76-A

51

98.765

0.504
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Acinetobacter baumannii D1279779

48.157

100

0.484
  pilC Vibrio cholerae strain A1552

40.704

98.272

0.4
  pilC Vibrio campbellii strain DS40M4

39.9

99.012

0.395
  pilC Thermus thermophilus HB27

39.109

99.753

0.39

Multiple sequence alignment