Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BPRO_RS04140 Genome accession   NC_007948
Coordinates   863324..865060 (+) Length   578 a.a.
NCBI ID   WP_011481799.1    Uniprot ID   -
Organism   Polaromonas sp. JS666     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858324..870060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPRO_RS04110 (Bpro_0836) proB 858876..860012 (-) 1137 WP_011481794.1 glutamate 5-kinase -
  BPRO_RS04115 (Bpro_0837) cgtA 860125..861210 (-) 1086 WP_011481795.1 Obg family GTPase CgtA -
  BPRO_RS04120 (Bpro_0838) rpmA 861329..861586 (-) 258 WP_011481796.1 50S ribosomal protein L27 -
  BPRO_RS04125 (Bpro_0839) rplU 861625..861936 (-) 312 WP_011481797.1 50S ribosomal protein L21 -
  BPRO_RS04130 (Bpro_0840) - 862114..863097 (+) 984 WP_011481798.1 polyprenyl synthetase family protein -
  BPRO_RS04140 (Bpro_0841) pilB 863324..865060 (+) 1737 WP_011481799.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BPRO_RS04145 (Bpro_0842) pilC 865142..866359 (+) 1218 WP_011481800.1 type II secretion system F family protein Machinery gene
  BPRO_RS04150 (Bpro_0843) - 866359..867216 (+) 858 WP_011481801.1 prepilin peptidase -
  BPRO_RS04155 (Bpro_0844) coaE 867221..867844 (+) 624 WP_011481802.1 dephospho-CoA kinase -
  BPRO_RS04160 (Bpro_0845) zapD 867888..868643 (+) 756 WP_011481803.1 cell division protein ZapD -
  BPRO_RS04165 (Bpro_0846) - 868685..868891 (+) 207 WP_011481804.1 DNA gyrase inhibitor YacG -
  BPRO_RS04170 (Bpro_0847) - 868986..869261 (+) 276 WP_011481805.1 hypothetical protein -
  BPRO_RS04175 (Bpro_0848) grxD 869314..869643 (-) 330 WP_011481806.1 Grx4 family monothiol glutaredoxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62518.03 Da        Isoelectric Point: 5.0770

>NTDB_id=25675 BPRO_RS04140 WP_011481799.1 863324..865060(+) (pilB) [Polaromonas sp. JS666]
MAAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSAFDLAHTMSIAFAAPLLDLEA
IDVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSDQEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAM
ESIIGDDFEFDESSVDSGAAEDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPP
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRILDPSSAKLGIDALGYEPVEKE
RLLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNIATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDP
DIIMVGEIRDIETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIP
RETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSALEIAAQAEAEGVRSLRQSGLS
KVKLGTTSLEEVLGCTNV

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=25675 BPRO_RS04140 WP_011481799.1 863324..865060(+) (pilB) [Polaromonas sp. JS666]
ATGGCCGCTGTCGATACTGCTATCAACGAACCGCCCGCCTTGGCATTGCCCGGATTGGGCCGGGCATTGGTGGTGGCAGG
CAAGCTGGGCCAGAAGGCCGCTGAAGACATTTTTCGCAAGGCCCAAAGCGGGCGCACCAGCTTCATTGCCGAGCTGACCG
GTTCAGGCGCCGTGTCTGCGTTTGACCTGGCGCACACGATGTCCATCGCGTTCGCCGCGCCTTTGCTCGATCTGGAGGCC
ATCGACGTCCAGCGTTTGCCCAAAGGCCTGCTCGACACCAAGATTTGCACGACTTACCGCATTGTCGTGCTGAGCAAGCG
CAATAACCGCCTGATCGTCGCAACGGCAGATCCCTCTGACCAGGAAGCGGCGGAAAAGATCAAGTTTTCCACCCAGATGG
GTGTGGACTGGGTGATTGCCGAGTATGACAAGCTGTCCAAACTGGTGGAGGCGTCGAGTACCACGGCTGCCGAGGCCATG
GAGAGCATCATCGGCGATGATTTCGAGTTCGATGAATCTTCGGTGGATTCAGGCGCTGCTGAAGACAGCGATGCCAGCGT
CTCTGAAGTCGAAGATGCACCGGTCGTCAAATTTCTCCAGAAGATGCTGCTCGATGCCTTCAGCATGCGGGCCTCTGATT
TGCACTTTGAGCCGTTCGAGCACACCTACCGTGTCCGTTTCCGGATCGACGGCGAATTGCGCGAAATTGCTTCGCCGCCC
GTGGCCATCAAGGAAAAACTCGCGGCACGTATCAAGGTGATCTCCAAGCTGGATATCTCGGAAAAGCGGGTGCCGCAGGA
TGGCAAGATGAAGCTCAAGATCGGCCCTGACCGCGTGATCGATTTTCGGGTCAGCACCTTGCCCACCATGTACGGTGAAA
AGGTCGTGATCCGTATTCTGGACCCGAGCAGTGCCAAACTGGGTATTGACGCGCTGGGTTACGAGCCGGTTGAGAAGGAG
CGCTTGCTGGCGGCCATCCAGCGGCCCTATGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACAGTGTCGCT
GTACACCTGCCTGAATATCCTGAACAAGCCGGGCGTCAACATTGCCACGGCTGAAGACCCTGCCGAGATCACCTTGCCAG
GCGTCAACCAGGTCAGCATGAACGACAAGGCGGGCATGACTTTCTCTGTCGCGCTCAAGGCCTTTTTGCGTCAGGATCCG
GACATCATCATGGTGGGTGAGATCCGCGACATCGAAACCGCCGACATCGCGATCAAGGCGGCCCAGACCGGCCACATGGT
CATGTCAACGCTGCATACCAACGACGCGCCAACCACCTTGACCCGCATGCGCAATATGGGCATTGCGCCCTTCAATATTG
CATCGAGCGTGATCCTGATCACGGCGCAGCGGCTGGCGCGGCGCCTGTGTGTTAACTGCAAGGCGCCCGCAGATATTCCG
CGCGAAACCTTGCTGGAGGCAGGATTCAAGGAAGAAGAAGTCGATGGTTCCTGGACGCCATATCGCCCGGTGGGCTGTTC
CATGTGTACAAATGGTTACAAGGGTCGCGTTGGCATCTACCAGGTCATGCCTATTTCCGAAGAGATCCAGCGGATAGTGT
TGCGCGACGGCAGCGCCCTTGAGATTGCGGCGCAGGCAGAAGCTGAGGGTGTCAGGAGTTTGCGCCAGTCCGGCCTGTCT
AAAGTTAAACTCGGCACGACCTCCCTGGAAGAGGTTTTGGGTTGCACCAATGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.68

96.367

0.517
  pilB Acinetobacter baylyi ADP1

51.937

98.27

0.51
  pilF Neisseria gonorrhoeae MS11

50.973

97.751

0.498
  pilB Legionella pneumophila strain ERS1305867

50.624

97.059

0.491
  pilB Vibrio campbellii strain DS40M4

43.85

97.059

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

44.364

95.156

0.422
  pilB Vibrio cholerae strain A1552

46.05

89.792

0.413
  pilB/pilB1 Synechocystis sp. PCC 6803

36.877

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.377

91.696

0.37
  pilF Thermus thermophilus HB27

37.766

97.578

0.369

Multiple sequence alignment