Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MFLA_RS05680 Genome accession   NC_007947
Coordinates   1155262..1156434 (-) Length   390 a.a.
NCBI ID   WP_011479333.1    Uniprot ID   Q1H2A8
Organism   Methylobacillus flagellatus KT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1150262..1161434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS05650 (Mfla_1105) dhaL 1150849..1151484 (-) 636 WP_011479326.1 dihydroxyacetone kinase subunit DhaL -
  MFLA_RS05655 (Mfla_1106) rpiA 1151485..1152177 (-) 693 WP_011479327.1 ribose-5-phosphate isomerase RpiA -
  MFLA_RS05660 (Mfla_1107) - 1152270..1153052 (+) 783 WP_011479329.1 TrmH family RNA methyltransferase -
  MFLA_RS05665 (Mfla_1108) hslO 1153064..1153945 (-) 882 WP_011479330.1 Hsp33 family molecular chaperone HslO -
  MFLA_RS05670 (Mfla_1109) gluQRS 1154025..1154924 (-) 900 WP_011479331.1 tRNA glutamyl-Q(34) synthetase GluQRS -
  MFLA_RS05675 (Mfla_1110) - 1154936..1155262 (-) 327 WP_011479332.1 DUF3301 domain-containing protein -
  MFLA_RS05680 (Mfla_1111) pilU 1155262..1156434 (-) 1173 WP_011479333.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MFLA_RS05685 (Mfla_1112) - 1156729..1157400 (+) 672 WP_011479334.1 hypothetical protein -
  MFLA_RS05690 (Mfla_1113) pap 1157563..1159056 (-) 1494 WP_011479335.1 polyphosphate:AMP phosphotransferase -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43779.11 Da        Isoelectric Point: 6.3431

>NTDB_id=25652 MFLA_RS05680 WP_011479333.1 1155262..1156434(-) (pilU) [Methylobacillus flagellatus KT]
MNQEQALAYMHTLLRSMLEKKASDLFISADFPPAMKIDGKMTPVTQQKLTGEHTRAFAHALMSDKQRQEFEQNKECNFAI
WPKDIGRFRVNVFIQQEKVGMVLRTITTQIPRFDDLGLPPVLKDVIMAKRGLVILVGGTGSGKSTTLAALIDYRNEHSHG
HIITVEDPVEYVHASKNCLITHREVGRDTNDWFSALKNTLRQAPDVILIGEIRDRETMEFALAFAETGHLCMATLHANSA
NQAIDRIINFFPEERHAQLHMDLSLNLRGFISQRLVPKKGGGRCAAIEILLNSPLVADLILKGETNMIKDAMAKSVELGM
QTFDQALFALCEEGRITEEDALRNADSYNELRLRFKLHGKHASAEDNSARIDSLSLHEDEPEEAPDSLKP

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=25652 MFLA_RS05680 WP_011479333.1 1155262..1156434(-) (pilU) [Methylobacillus flagellatus KT]
ATGAATCAGGAACAGGCTCTAGCCTATATGCATACGCTGTTGCGCTCCATGCTGGAGAAGAAGGCATCTGACCTGTTCAT
CTCCGCGGACTTTCCGCCGGCAATGAAGATAGACGGCAAAATGACGCCGGTGACCCAGCAGAAGCTGACCGGCGAGCACA
CCAGAGCATTTGCCCATGCGCTCATGAGCGACAAGCAGCGCCAGGAATTCGAACAGAACAAGGAATGCAATTTCGCTATC
TGGCCAAAAGACATAGGCCGCTTCCGCGTCAACGTCTTCATCCAACAGGAAAAAGTCGGTATGGTGTTGCGCACCATCAC
GACACAGATCCCGCGCTTCGATGATCTGGGCCTGCCACCCGTGCTCAAGGACGTCATCATGGCCAAGCGCGGCCTGGTGA
TCCTCGTCGGCGGTACCGGCTCGGGCAAATCCACCACCCTCGCGGCCTTGATCGACTACCGCAACGAACACAGCCATGGC
CACATCATCACCGTGGAAGACCCGGTGGAATATGTGCACGCGAGCAAAAACTGCCTGATCACACACCGCGAAGTCGGTCG
CGACACCAACGATTGGTTCAGCGCGCTCAAGAACACTCTGCGCCAGGCCCCGGATGTCATCCTCATCGGCGAGATCCGGG
ACCGCGAAACCATGGAATTCGCCCTGGCATTTGCCGAGACCGGCCACCTGTGCATGGCGACGCTGCATGCCAACAGCGCC
AACCAGGCTATCGACCGCATCATCAATTTCTTCCCGGAAGAGCGCCATGCGCAGCTGCATATGGACTTGTCGCTCAACCT
GCGCGGGTTCATCTCGCAACGCCTGGTGCCCAAGAAAGGGGGCGGACGCTGCGCTGCGATCGAAATCCTGCTCAACTCTC
CCTTGGTCGCCGACCTCATCCTCAAGGGAGAAACCAATATGATCAAGGACGCCATGGCGAAATCGGTCGAGCTGGGCATG
CAGACCTTCGACCAGGCCTTGTTTGCACTGTGCGAGGAAGGCCGCATCACAGAAGAGGACGCATTGCGCAATGCTGACTC
CTACAACGAGCTGCGCCTGCGCTTCAAGCTGCACGGCAAGCATGCCTCTGCGGAGGACAATAGCGCGCGCATCGACAGCC
TTTCCCTGCACGAAGACGAACCCGAAGAAGCACCCGACTCACTGAAGCCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1H2A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.219

95.897

0.549
  pilU Acinetobacter baylyi ADP1

55.22

93.333

0.515
  pilU Vibrio cholerae strain A1552

54.598

89.231

0.487
  pilT Pseudomonas aeruginosa PAK

43.544

85.385

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.413

85.641

0.372
  pilT Acinetobacter baumannii strain A118

43.465

84.359

0.367
  pilT Acinetobacter baumannii D1279779

43.465

84.359

0.367
  pilT Acinetobacter nosocomialis M2

43.465

84.359

0.367
  pilT Acinetobacter baylyi ADP1

43.827

83.077

0.364

Multiple sequence alignment