Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   CTW00_RS03535 Genome accession   NZ_CP024843
Coordinates   714365..715306 (+) Length   313 a.a.
NCBI ID   WP_003098801.1    Uniprot ID   -
Organism   Streptococcus iniae strain FP5228     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 709365..720306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTW00_RS03525 (CTW00_00713) rpoC 710188..713820 (+) 3633 WP_003098797.1 DNA-directed RNA polymerase subunit beta' -
  CTW00_RS03530 (CTW00_00714) - 713939..714304 (+) 366 WP_003098799.1 DUF1033 family protein -
  CTW00_RS03535 (CTW00_00715) comYA 714365..715306 (+) 942 WP_003098801.1 competence type IV pilus ATPase ComGA Machinery gene
  CTW00_RS03540 (CTW00_00716) comYB 715257..716273 (+) 1017 WP_003098804.1 competence type IV pilus assembly protein ComGB Machinery gene
  CTW00_RS03545 (CTW00_00717) comGC/cglC 716274..716600 (+) 327 WP_003098806.1 competence type IV pilus major pilin ComGC Machinery gene
  CTW00_RS10430 (CTW00_00718) comGD 716560..716988 (+) 429 WP_003098807.1 competence type IV pilus minor pilin ComGD -
  CTW00_RS03555 comGE 716960..717253 (+) 294 WP_031239176.1 competence type IV pilus minor pilin ComGE -
  CTW00_RS03560 (CTW00_00719) comGF 717282..717662 (+) 381 WP_003098811.1 competence type IV pilus minor pilin ComGF -
  CTW00_RS03565 (CTW00_00720) comGG 717652..718005 (+) 354 WP_003098812.1 competence type IV pilus minor pilin ComGG -
  CTW00_RS03570 (CTW00_00721) comYH 718067..719023 (+) 957 WP_100185175.1 class I SAM-dependent methyltransferase Machinery gene
  CTW00_RS03575 (CTW00_00722) - 719080..720276 (+) 1197 WP_003098816.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35927.07 Da        Isoelectric Point: 7.4276

>NTDB_id=255810 CTW00_RS03535 WP_003098801.1 714365..715306(+) (comYA) [Streptococcus iniae strain FP5228]
MIQSLGKEVIKMADDLKAQDIYIIPEGDDYKIHMRIDGERRRIESFDGAQMPQLISHFKYLSGMNVGEKRRCQLGACDYQ
YGAKNDISLRLSTVGDYRGKESLVIRLLHHQNRSLSFWFDREKELAEQVTRRGLYLFSGPVGSGKTSLMYQLLAKDSKQK
QIITIEDPVEIKDTRMLQLQVNDSIGTTYDNLIKLSLRHRPDVLIIGEIRDTETARAAIRASLTGSVVFSTIHAKSIKGV
YERLIELGISREEVTQTLQLVVYQRLLGGRGLIDFTRKGFENYQQEQWNKDLDQLFADGYLTEQEIQTEKIIS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=255810 CTW00_RS03535 WP_003098801.1 714365..715306(+) (comYA) [Streptococcus iniae strain FP5228]
ATGATTCAAAGCTTAGGAAAAGAAGTGATTAAAATGGCAGATGACTTGAAGGCTCAGGACATATATATTATTCCAGAGGG
CGATGATTACAAGATTCATATGCGTATTGATGGAGAAAGAAGACGCATTGAATCTTTTGATGGCGCACAGATGCCTCAAC
TCATCAGTCACTTTAAATATCTATCGGGAATGAATGTTGGCGAAAAACGTCGATGCCAACTTGGCGCCTGTGATTACCAA
TATGGTGCTAAAAATGACATATCGCTTCGACTATCAACAGTTGGAGATTATCGTGGGAAGGAAAGTTTAGTAATCAGGCT
TTTACACCACCAAAACAGGTCCTTATCTTTTTGGTTTGATAGGGAAAAGGAATTGGCCGAGCAAGTCACCAGACGAGGAC
TTTATTTATTTTCAGGCCCCGTTGGTTCCGGTAAAACGAGTTTAATGTATCAACTTCTAGCAAAGGATTCTAAACAAAAG
CAAATCATCACTATTGAAGATCCCGTTGAGATTAAGGACACAAGAATGCTACAGTTGCAAGTAAATGACAGTATTGGAAC
GACATATGATAATTTAATTAAATTATCTTTGCGTCATCGTCCAGATGTTCTCATTATTGGTGAAATTAGAGACACTGAGA
CAGCTAGAGCAGCCATTAGAGCAAGTTTAACTGGTAGTGTCGTCTTTTCCACCATACATGCTAAAAGCATCAAGGGTGTC
TATGAGAGATTAATTGAATTAGGAATCTCTAGAGAAGAAGTGACACAAACACTTCAACTTGTTGTTTATCAACGGCTACT
TGGTGGTCGAGGCTTAATTGACTTTACACGAAAGGGGTTTGAAAACTATCAACAAGAGCAGTGGAATAAAGACTTGGATC
AACTTTTTGCGGATGGATATCTCACTGAGCAAGAAATCCAAACAGAGAAAATTATCAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

62.058

99.361

0.617
  comYA Streptococcus mutans UA159

62.058

99.361

0.617
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.29

99.042

0.607
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comYA Streptococcus gordonii str. Challis substr. CH1

58.842

99.361

0.585
  comGA Lactococcus lactis subsp. cremoris KW2

53.376

99.361

0.53

Multiple sequence alignment