Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   CTW00_RS03540 Genome accession   NZ_CP024843
Coordinates   715257..716273 (+) Length   338 a.a.
NCBI ID   WP_003098804.1    Uniprot ID   -
Organism   Streptococcus iniae strain FP5228     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710257..721273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTW00_RS03530 (CTW00_00714) - 713939..714304 (+) 366 WP_003098799.1 DUF1033 family protein -
  CTW00_RS03535 (CTW00_00715) comYA 714365..715306 (+) 942 WP_003098801.1 competence type IV pilus ATPase ComGA Machinery gene
  CTW00_RS03540 (CTW00_00716) comYB 715257..716273 (+) 1017 WP_003098804.1 competence type IV pilus assembly protein ComGB Machinery gene
  CTW00_RS03545 (CTW00_00717) comGC/cglC 716274..716600 (+) 327 WP_003098806.1 competence type IV pilus major pilin ComGC Machinery gene
  CTW00_RS10430 (CTW00_00718) comGD 716560..716988 (+) 429 WP_003098807.1 competence type IV pilus minor pilin ComGD -
  CTW00_RS03555 comGE 716960..717253 (+) 294 WP_031239176.1 competence type IV pilus minor pilin ComGE -
  CTW00_RS03560 (CTW00_00719) comGF 717282..717662 (+) 381 WP_003098811.1 competence type IV pilus minor pilin ComGF -
  CTW00_RS03565 (CTW00_00720) comGG 717652..718005 (+) 354 WP_003098812.1 competence type IV pilus minor pilin ComGG -
  CTW00_RS03570 (CTW00_00721) comYH 718067..719023 (+) 957 WP_100185175.1 class I SAM-dependent methyltransferase Machinery gene
  CTW00_RS03575 (CTW00_00722) - 719080..720276 (+) 1197 WP_003098816.1 acetate kinase -
  CTW00_RS03580 (CTW00_00723) - 720399..720602 (+) 204 WP_003098819.1 helix-turn-helix transcriptional regulator -
  CTW00_RS03585 (CTW00_00724) - 720615..721067 (+) 453 WP_003098820.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 39194.38 Da        Isoelectric Point: 9.6521

>NTDB_id=255811 CTW00_RS03540 WP_003098804.1 715257..716273(+) (comYB) [Streptococcus iniae strain FP5228]
MDISLSKKSKQRKLSAKKQYQLMQLFHNLFASGFSLSEIVAFLEKSHLLEAKYLDRIKENLISGRSLAEMTRSLGYPDSI
VTQISFADIHGNTKESLFKIMHYLDKVAQVRKKTLEVLTYPLILLSFLIAIMFGLRHYLLPQIEESNGLTQFLTYFPLVF
LIFLILGLLLTFYLSIRWRKHSQLKQVKQFSRIPFLKDYISLYITAYFAREWGSLMGQGLELSSILTVMAKEQSPLVKEI
GQDMQAYFLEGGALHDKVLQYPFFQKELSLMIEYGDIKAKLGQELEIYAQLTWERFFSRLFQATQWIQPIIFLFVALIIV
CIYAAMLLPMYHSIGGSI

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=255811 CTW00_RS03540 WP_003098804.1 715257..716273(+) (comYB) [Streptococcus iniae strain FP5228]
ATGGATATCTCACTGAGCAAGAAATCCAAACAGAGAAAATTATCAGCTAAAAAGCAGTATCAATTAATGCAACTCTTTCA
TAATTTATTTGCAAGTGGTTTTAGCTTATCTGAAATAGTGGCATTTTTAGAAAAAAGTCACTTATTGGAAGCAAAATACC
TTGATAGAATAAAAGAAAACCTCATCAGTGGACGAAGTTTAGCAGAAATGACTAGAAGTTTAGGTTATCCTGACAGTATT
GTAACGCAAATTAGTTTTGCAGATATTCATGGCAATACCAAGGAAAGCTTGTTTAAAATTATGCATTACCTTGATAAAGT
GGCACAAGTCAGGAAAAAAACATTAGAAGTCCTAACTTATCCTCTTATTTTGCTTAGTTTTTTAATAGCCATCATGTTTG
GCTTACGCCATTATCTGCTTCCTCAAATAGAGGAGTCTAATGGGCTTACTCAATTTTTGACTTACTTCCCTTTAGTTTTT
TTGATTTTCTTAATATTGGGATTGCTACTCACATTTTACTTGAGCATTAGGTGGCGAAAACACTCACAATTAAAACAAGT
CAAGCAGTTTAGTCGGATACCTTTTTTAAAAGACTATATTAGCCTATATATAACTGCCTATTTTGCGCGTGAATGGGGAA
GTTTAATGGGACAAGGCCTTGAGTTGAGTTCAATACTAACCGTTATGGCAAAAGAACAATCTCCTCTGGTAAAAGAAATC
GGCCAAGATATGCAGGCCTATTTTTTAGAGGGTGGTGCTTTGCATGACAAAGTGTTACAATACCCATTTTTTCAAAAGGA
ATTAAGCTTAATGATTGAATATGGAGATATCAAGGCTAAATTAGGTCAAGAATTAGAAATTTATGCTCAACTAACATGGG
AAAGATTCTTTAGCCGCTTATTTCAAGCGACACAATGGATTCAACCTATTATATTCTTATTTGTTGCACTTATTATTGTG
TGCATTTACGCGGCAATGCTTTTGCCCATGTATCATTCAATTGGAGGTTCTATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(208-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

52.252

98.521

0.515
  comYB Streptococcus mutans UA159

52.252

98.521

0.515
  comGB/cglB Streptococcus mitis NCTC 12261

48.673

100

0.488
  comGB/cglB Streptococcus mitis SK321

48.204

98.817

0.476
  comYB Streptococcus gordonii str. Challis substr. CH1

48.048

98.521

0.473
  comGB/cglB Streptococcus pneumoniae Rx1

46.407

98.817

0.459
  comGB/cglB Streptococcus pneumoniae D39

46.407

98.817

0.459
  comGB/cglB Streptococcus pneumoniae R6

46.407

98.817

0.459
  comGB/cglB Streptococcus pneumoniae TIGR4

46.407

98.817

0.459
  comGB Lactococcus lactis subsp. cremoris KW2

43.544

98.521

0.429

Multiple sequence alignment