Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CT122_RS26030 Genome accession   NZ_CP024712
Coordinates   5556269..5557486 (+) Length   405 a.a.
NCBI ID   WP_099978043.1    Uniprot ID   A0AAU8XM69
Organism   Pseudomonas syringae pv. actinidiae strain MAFF212063     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5551269..5562486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CT122_RS26015 (CT122_26015) - 5552476..5553753 (-) 1278 WP_232538452.1 O-antigen ligase family protein -
  CT122_RS26020 (CT122_26020) - 5553954..5554346 (-) 393 WP_020325889.1 pilin -
  CT122_RS26025 (CT122_26025) pilB 5554572..5556266 (+) 1695 WP_003381201.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CT122_RS26030 (CT122_26030) pilC 5556269..5557486 (+) 1218 WP_099978043.1 type II secretion system F family protein Machinery gene
  CT122_RS26035 (CT122_26035) pilD 5557488..5558360 (+) 873 WP_020305348.1 prepilin peptidase Machinery gene
  CT122_RS26040 (CT122_26040) coaE 5558357..5558980 (+) 624 WP_099978042.1 dephospho-CoA kinase -
  CT122_RS26045 (CT122_26045) yacG 5558977..5559186 (+) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  CT122_RS26050 (CT122_26050) - 5559453..5559779 (-) 327 WP_099978041.1 hypothetical protein -
  CT122_RS26055 (CT122_26055) - 5559976..5560605 (+) 630 WP_003381213.1 DUF1780 domain-containing protein -
  CT122_RS26060 (CT122_26060) - 5560602..5561135 (+) 534 WP_099978040.1 MOSC domain-containing protein -
  CT122_RS34465 - 5561020..5561256 (+) 237 WP_225877647.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44264.24 Da        Isoelectric Point: 10.1599

>NTDB_id=255195 CT122_RS26030 WP_099978043.1 5556269..5557486(+) (pilC) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
MASKAVKISIYTWEGVDKKGGKLSGEVSGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIAEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVALFFVSFFMFK
RAYKQSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDNMTSLMEPMIMAILGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=255195 CT122_RS26030 WP_099978043.1 5556269..5557486(+) (pilC) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
ATGGCCAGCAAGGCAGTTAAAATCAGTATTTACACATGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGAGAAGT
GAGCGGGCACAACCTCGCGCTCGTGAAGGCTCAGTTACGCAAGCAAGGAATCAATTTCACCAAAGTACGTAAAAAGCCCG
TATCGATTTTTGGCAAAGGTAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCCCGGCAGATGGCTACCATGATGAAG
GCAGGAGTGCCACTGCTGCAGTCATTTGACATCATTGCCGAAGGTGCAGAAAACCCGAACATGCGTGCGCTGGTCGGCTC
TCTAAAGCAGGAGGTTTCAGCAGGGAACAGCTTTGCGACGGCTCTGCGAAAGAAACCGGAATATTTCGACGACCTGTTCT
GCAATCTGGTCGATGCCGGTGAGCAAGCGGGGGCGCTCGAAAGCCTGCTGGACCGCGTGGCGAGCTACAAGGAAAAGACG
GAAAGGCTAAAGGCCAAAATCAAGAAGGCGATGACTTATCCTACCGCCGTATTAATTGTTGCTTTTATCGTGTCAGGCAT
TTTGCTGATTAAGGTCGTGCCGCAGTTCCAGTCTGTTTTCGCTGGTTTTAACGCAGAATTACCAGCGTTCACGCTGATGG
TCATCGCCCTTTCGGAAGTCGTTCAACAGTGGTGGCTGGCAATAGTTGCGTTATTTTTTGTCAGCTTTTTCATGTTCAAG
CGGGCTTACAAACAGTCTCAAAAGTTTCGTGACAGCCTCGACCGCTTCCTTCTGAAAGTCCCCATTATCGGACCACTGAT
TTTCAAGTCGTCCGTCGCGCGCTATGCGCGGACCCTTGCGACAACCTTTGCCGCAGGTGTCCCGCTGGTGGAGGCACTTG
ACTCGGTCGCCGGCGCCACCGGTAACGTCGTATTCAGGAACGCAGTCAACCAGGTCAAACAGGATGTCTCCACTGGTATG
CAATTGAATTTCTCGATGCGTTCAACGGGTGTATTTCCAAGTCTGGCGATACAAATGACAGCAATCGGCGAAGAGTCCGG
TGCGCTTGATGCCATGCTCGATAAAGTCGCGACCTACTATGAAGAAGAAGTCGACAACATGGTCGACAATATGACCAGCC
TGATGGAACCGATGATCATGGCGATACTGGGTGTCATCGTCGGCGGCCTGGTTATTGCCATGTACCTCCCCATCTTCAAA
CTCGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

59.158

99.753

0.59
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilC Vibrio campbellii strain DS40M4

41.769

100

0.42
  pilG Neisseria gonorrhoeae MS11

42.821

98.025

0.42
  pilG Neisseria meningitidis 44/76-A

42.821

98.025

0.42
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Thermus thermophilus HB27

37.624

99.753

0.375

Multiple sequence alignment