Detailed information NTG0255196

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CT122_RS26035
• Genome accession: NZ_CP024712
• Coordinates: 5557488..5558360 (+)
• Length: 290 a.a.
• NCBI ID: WP_020305348.1
• Uniprot ID: A0A2G9L7U9
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF212063
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5552488..5563360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CT122_RS26020 (CT122_26020)	-	5553954..5554346 (-)	393	WP_020325889.1	pilin	-
CT122_RS26025 (CT122_26025)	pilB	5554572..5556266 (+)	1695	WP_003381201.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CT122_RS26030 (CT122_26030)	pilC	5556269..5557486 (+)	1218	WP_099978043.1	type II secretion system F family protein	Machinery gene
CT122_RS26035 (CT122_26035)	pilD	5557488..5558360 (+)	873	WP_020305348.1	prepilin peptidase	Machinery gene
CT122_RS26040 (CT122_26040)	coaE	5558357..5558980 (+)	624	WP_099978042.1	dephospho-CoA kinase	-
CT122_RS26045 (CT122_26045)	yacG	5558977..5559186 (+)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
CT122_RS26050 (CT122_26050)	-	5559453..5559779 (-)	327	WP_099978041.1	hypothetical protein	-
CT122_RS26055 (CT122_26055)	-	5559976..5560605 (+)	630	WP_003381213.1	DUF1780 domain-containing protein	-
CT122_RS26060 (CT122_26060)	-	5560602..5561135 (+)	534	WP_099978040.1	MOSC domain-containing protein	-
CT122_RS34465	-	5561020..5561256 (+)	237	WP_225877647.1	DUF3094 family protein	-
CT122_RS26070 (CT122_26070)	-	5561352..5562650 (+)	1299	WP_099978039.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31988.27 Da
• Isoelectric Point: 8.3782

>NTDB_id=255196 CT122_RS26035 WP_020305348.1 5557488..5558360(+) (pilD) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKAQSREMLGLPAVPDQPVFNLILPHSSCPHCAHKIR
PWENLPIISYLLLRGKCSHCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=255196 CT122_RS26035 WP_020305348.1 5557488..5558360(+) (pilD) [Pseudomonas syringae pv. actinidiae strain MAFF212063]
ATGCCCCTCCTCGACCTCCTGGCAAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCCTGCTCGGCCTCATCGTCGGAAG
CTTCCTCAACGTAGTCGTTTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGCGCAATCACGGGAAATGCTCGGCC
TCCCTGCCGTACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCCAGTTGCCCGCACTGCGCGCACAAGATCCGC
CCATGGGAAAATCTGCCGATCATCAGTTACCTGTTGCTGCGCGGCAAATGCTCACACTGCAAAGCACCGATCAGCAAGCG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTATCGGCCTACGTCGCCTGGCACTTCGGCTTTGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTGCCTGACTCTCTGGTG
CTGCCACTTCTGTGGCTGGGCCTGATCGTTAATGCCTTTGGGCTGTTCACCTCGCTGAACGATGCGCTGTGGGGTGCGGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAATTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGTTGGTGGGT
GCGGTACTTGGGGTGATCATGATGCGGGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A2G9L7U9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.77	97.586	0.534
pilD	Vibrio campbellii strain DS40M4	51.59	97.586	0.503
pilD	Acinetobacter baumannii D1279779	51.625	95.517	0.493
pilD	Acinetobacter nosocomialis M2	51.264	95.517	0.49
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483