Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CR152_RS08190 Genome accession   NZ_CP024608
Coordinates   1817229..1818455 (-) Length   408 a.a.
NCBI ID   WP_099874471.1    Uniprot ID   A0A2D2DHN7
Organism   Massilia violaceinigra strain B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1812229..1823455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR152_RS08165 (CR152_08140) yacG 1813083..1813268 (-) 186 WP_099874466.1 DNA gyrase inhibitor YacG -
  CR152_RS08170 (CR152_08145) zapD 1813282..1814037 (-) 756 WP_099874467.1 cell division protein ZapD -
  CR152_RS08175 (CR152_08150) coaE 1814099..1814755 (-) 657 WP_099874468.1 dephospho-CoA kinase -
  CR152_RS08180 (CR152_08155) - 1814756..1815649 (-) 894 WP_099874469.1 A24 family peptidase -
  CR152_RS08185 (CR152_08160) - 1815760..1816890 (-) 1131 WP_099874470.1 hypothetical protein -
  CR152_RS08190 (CR152_08165) pilC 1817229..1818455 (-) 1227 WP_099874471.1 type II secretion system F family protein Machinery gene
  CR152_RS08195 (CR152_08170) pilB 1818467..1820194 (-) 1728 WP_099874472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CR152_RS08200 (CR152_08175) - 1820406..1821686 (-) 1281 WP_099874473.1 HlyC/CorC family transporter -
  CR152_RS08205 (CR152_08180) - 1821888..1822448 (-) 561 WP_099874474.1 ATP-dependent Clp protease proteolytic subunit -
  CR152_RS08210 (CR152_08185) uraH 1822516..1822869 (-) 354 WP_099874475.1 hydroxyisourate hydrolase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44186.19 Da        Isoelectric Point: 10.0526

>NTDB_id=254197 CR152_RS08190 WP_099874471.1 1817229..1818455(-) (pilC) [Massilia violaceinigra strain B2]
MALAQSKTAAAVKESVYAWEGKDKTGKTVRGELRAGGEAVVNVTLRRQGVMVTKVKKKTYRSGKKITDKDISLFTRQLAT
MMKAGVPLLQSFDIVSKGHANPSMAKLILDLRSDIETGTSLNNAFRKYPLYFDPLFCNLVGAGEQAGILEDLLTRLAIYK
EKTLAIKAKIKSALMYPVSILGIAFIVTAVIMIWVVPAFKSVFTSFGANLPTPTVIVMDISDFFVSYWWAIFGGLFAGLY
FFFQTWQRSAKMQRFMDLALLKAPIFGSVIRKAAIARWTRTLATMFAAGVPLVEALDSVGGAAGNALYLEATKRIQNEVS
TGTSLTAAMQNANVFPPIVTQMVAIGEESGALDSMLGKVAGFFEDEVDEAVATLASLMEPLIMVILGVIIGGLVVAMYLP
IFKLGAVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=254197 CR152_RS08190 WP_099874471.1 1817229..1818455(-) (pilC) [Massilia violaceinigra strain B2]
ATGGCACTCGCTCAATCTAAAACCGCCGCCGCCGTCAAGGAGTCGGTCTACGCCTGGGAAGGCAAGGACAAGACCGGCAA
GACCGTGCGCGGCGAACTGCGCGCCGGCGGCGAAGCCGTGGTCAATGTGACCTTGCGGCGCCAGGGTGTCATGGTGACCA
AGGTAAAGAAAAAGACTTATCGCAGCGGCAAGAAGATCACGGACAAGGATATTTCCCTGTTCACGCGCCAGCTGGCCACG
ATGATGAAGGCCGGCGTGCCGCTGCTGCAATCGTTCGACATCGTCAGCAAGGGCCACGCGAATCCATCGATGGCCAAGCT
GATTCTCGACCTGCGTTCGGACATCGAGACCGGTACCAGCCTGAACAATGCTTTCCGCAAGTACCCGCTCTACTTCGACC
CGCTGTTCTGTAATCTGGTCGGCGCCGGCGAACAGGCCGGTATTCTGGAAGACTTGCTCACGCGTCTGGCGATCTACAAG
GAAAAAACCCTGGCCATCAAGGCCAAGATCAAGTCGGCGCTGATGTATCCGGTGTCGATCCTGGGCATCGCCTTCATCGT
CACCGCCGTGATCATGATCTGGGTGGTACCGGCGTTCAAATCGGTGTTTACCAGCTTCGGCGCCAACCTGCCGACGCCGA
CCGTGATCGTGATGGATATTTCCGACTTCTTCGTCTCGTACTGGTGGGCGATTTTCGGCGGTCTGTTTGCCGGCCTGTAC
TTCTTCTTCCAGACCTGGCAGCGTTCGGCCAAGATGCAGCGCTTCATGGACCTGGCCCTGCTCAAGGCGCCGATTTTCGG
CAGCGTGATCCGCAAGGCGGCCATTGCCCGCTGGACCCGGACCCTGGCCACCATGTTCGCGGCCGGCGTGCCGCTGGTCG
AAGCGCTCGACTCGGTCGGCGGCGCGGCCGGCAATGCCCTGTACCTGGAAGCGACCAAGCGCATCCAGAACGAGGTGAGC
ACCGGTACCAGCCTGACCGCCGCCATGCAGAACGCCAATGTGTTTCCGCCGATCGTGACCCAGATGGTCGCCATCGGTGA
AGAATCGGGCGCGCTCGATTCGATGCTGGGCAAGGTGGCCGGCTTCTTCGAAGATGAGGTCGACGAGGCCGTCGCCACCC
TGGCGTCGCTGATGGAACCGTTGATCATGGTGATTTTGGGCGTGATCATCGGCGGCTTGGTCGTCGCCATGTACCTGCCG
ATCTTCAAACTGGGCGCGGTGGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D2DHN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

51.87

98.284

0.51
  pilG Neisseria gonorrhoeae MS11

51.88

97.794

0.507
  pilG Neisseria meningitidis 44/76-A

51.378

97.794

0.502
  pilC Acinetobacter baumannii D1279779

51.134

97.304

0.498
  pilC Legionella pneumophila strain ERS1305867

50.758

97.059

0.493
  pilC Acinetobacter baylyi ADP1

50

97.549

0.488
  pilC Vibrio cholerae strain A1552

38.958

98.775

0.385
  pilC Vibrio campbellii strain DS40M4

39.447

97.549

0.385
  pilC Thermus thermophilus HB27

37.343

97.794

0.365

Multiple sequence alignment