Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CR152_RS08195 Genome accession   NZ_CP024608
Coordinates   1818467..1820194 (-) Length   575 a.a.
NCBI ID   WP_099874472.1    Uniprot ID   A0A2D2DHP1
Organism   Massilia violaceinigra strain B2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1813467..1825194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR152_RS08175 (CR152_08150) coaE 1814099..1814755 (-) 657 WP_099874468.1 dephospho-CoA kinase -
  CR152_RS08180 (CR152_08155) - 1814756..1815649 (-) 894 WP_099874469.1 A24 family peptidase -
  CR152_RS08185 (CR152_08160) - 1815760..1816890 (-) 1131 WP_099874470.1 hypothetical protein -
  CR152_RS08190 (CR152_08165) pilC 1817229..1818455 (-) 1227 WP_099874471.1 type II secretion system F family protein Machinery gene
  CR152_RS08195 (CR152_08170) pilB 1818467..1820194 (-) 1728 WP_099874472.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CR152_RS08200 (CR152_08175) - 1820406..1821686 (-) 1281 WP_099874473.1 HlyC/CorC family transporter -
  CR152_RS08205 (CR152_08180) - 1821888..1822448 (-) 561 WP_099874474.1 ATP-dependent Clp protease proteolytic subunit -
  CR152_RS08210 (CR152_08185) uraH 1822516..1822869 (-) 354 WP_099874475.1 hydroxyisourate hydrolase -
  CR152_RS08215 (CR152_08190) puuE 1822991..1823923 (+) 933 WP_099874476.1 allantoinase PuuE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62592.51 Da        Isoelectric Point: 5.5798

>NTDB_id=254198 CR152_RS08195 WP_099874472.1 1818467..1820194(-) (pilB) [Massilia violaceinigra strain B2]
MAAVLPNTTPGTPMPGLARALMQAGRLSAQQADTLHKKSLVDKSPFIDTLLLTGTFDARTLAAFCAETFGYPLMDLQSFN
IDALPPNAIDGKLMQTQRVIALAKRGNKMSVAISDPTNIQALDQIKFQSEASVEPVIVLHDALLILLASLGRNAEQNLND
MVGDQEDIQFGEDDAPAVVDQAAAADIEDAPIVKFLNKILMDAINLGASDIHFEPFEKFYRIRLRVDGMLKEHAQPPITI
RDKLVSRIKVLSKLDISEKRVPQDGRMRLILSPTKAIDFRVSTCPTLFGEKTVMRILDATQAQMGIDALGYDPDQKELLL
EAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNINERSGLTFPVALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVIMITAQRLARRLCTCKKPVETSREIM
LAAGFREEELEGGWKPYGPVGCDRCLGGGYKGRVGIYQIMPITSAIEKIILAHGNSMEIAAQSELEGVKSLRRSGLMKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=254198 CR152_RS08195 WP_099874472.1 1818467..1820194(-) (pilB) [Massilia violaceinigra strain B2]
ATGGCTGCAGTCCTCCCCAACACGACCCCCGGAACGCCCATGCCGGGCCTGGCCCGCGCCCTGATGCAGGCTGGCCGCCT
CAGTGCGCAGCAAGCCGATACGCTGCACAAGAAGTCGCTGGTCGACAAGAGTCCGTTCATCGATACCCTGCTGCTCACGG
GTACCTTCGACGCGCGCACCCTGGCCGCCTTTTGCGCGGAAACCTTCGGCTACCCGCTGATGGATTTGCAGAGCTTTAAT
ATCGACGCCCTGCCGCCGAACGCCATCGACGGCAAGTTGATGCAGACCCAGCGCGTGATCGCCCTGGCCAAGCGCGGCAA
CAAGATGTCGGTCGCCATTTCCGACCCCACCAATATCCAGGCGCTCGACCAGATCAAGTTCCAGAGCGAAGCGTCGGTCG
AGCCGGTGATCGTGCTGCACGACGCCCTGCTGATCCTGCTGGCCAGCCTGGGCCGCAACGCCGAACAGAATCTGAACGAC
ATGGTCGGCGACCAGGAAGACATCCAGTTCGGCGAGGACGATGCGCCCGCCGTGGTCGACCAGGCCGCGGCCGCCGATAT
CGAAGATGCGCCGATCGTCAAGTTCCTCAACAAGATCCTGATGGACGCGATCAACCTCGGCGCGTCCGATATCCACTTTG
AGCCATTCGAAAAGTTTTACCGCATCCGCCTGCGCGTGGATGGCATGCTGAAAGAACACGCCCAGCCGCCGATCACCATC
CGCGACAAGCTGGTCTCGCGCATCAAGGTACTCTCCAAGCTCGACATTTCCGAAAAGCGCGTGCCGCAGGATGGCCGCAT
GCGCCTGATCCTCTCGCCCACCAAGGCGATCGACTTCCGGGTCAGCACCTGCCCCACCCTGTTCGGCGAAAAGACCGTCA
TGCGGATTCTGGACGCCACCCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGATCCGGACCAGAAGGAATTGCTGCTC
GAAGCGATCCAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCCGGCAAGACGGTGTCGCTGTACACCTG
CCTGAACGTGATCAACAAGCCGGGCATTAACATTTCGACGGCGGAAGACCCGGCCGAGATTAACTTGCCGGGCGTGAACC
AGGTCAACATCAACGAACGCTCCGGCCTGACCTTTCCGGTCGCGCTCAAGTCCTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCCCAGACCGGCCACATGGTGTTCTCGAC
CCTGCACACCAACGATGCGCCGTCGACCCTGACGCGTTTGATGAACATGGGCGTGGCGCCGTTTAACATCGCCTCGTCGG
TGATCATGATCACGGCGCAGCGCCTGGCGCGGCGCTTGTGCACCTGCAAGAAGCCGGTGGAAACCAGCCGCGAAATCATG
CTCGCGGCCGGCTTTCGGGAAGAGGAACTGGAAGGCGGCTGGAAGCCTTACGGTCCGGTCGGCTGCGACCGTTGCCTGGG
CGGCGGTTACAAGGGCCGGGTCGGCATCTACCAGATCATGCCGATCACCTCGGCGATCGAAAAAATCATTCTGGCGCACG
GCAATTCGATGGAAATTGCCGCGCAATCGGAGCTGGAGGGCGTCAAATCCCTGCGCCGTTCCGGCCTGATGAAAGTAAAG
CAAGGCTTGACCAGCCTTGAAGAAGTTCTTGGCTGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D2DHP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.887

98.435

0.53
  pilB Acinetobacter baylyi ADP1

52.982

99.13

0.525
  pilF Neisseria gonorrhoeae MS11

52.575

97.913

0.515
  pilB Legionella pneumophila strain ERS1305867

48.399

97.739

0.473
  pilB Vibrio cholerae strain A1552

44.938

97.913

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

44.265

97.043

0.43
  pilB Vibrio campbellii strain DS40M4

43.339

97.913

0.424
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.943

98.087

0.372
  pilF Thermus thermophilus HB27

39.626

93.043

0.369

Multiple sequence alignment