Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CPC19_RS11165 Genome accession   NZ_CP023664
Coordinates   2299189..2300898 (+) Length   569 a.a.
NCBI ID   WP_016390929.1    Uniprot ID   -
Organism   Cycloclasticus sp. PY97N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2294189..2305898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPC19_RS11145 (CPC19_11145) - 2295322..2296128 (-) 807 WP_016390925.1 FkbM family methyltransferase -
  CPC19_RS11150 (CPC19_11150) - 2296146..2297246 (-) 1101 WP_016390926.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  CPC19_RS11155 (CPC19_11155) - 2297243..2297830 (-) 588 WP_016390927.1 acyltransferase -
  CPC19_RS11160 (CPC19_11160) - 2297838..2298893 (-) 1056 WP_016390928.1 Gfo/Idh/MocA family protein -
  CPC19_RS11165 (CPC19_11165) pilB 2299189..2300898 (+) 1710 WP_016390929.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CPC19_RS11170 (CPC19_11170) pilC 2300904..2302124 (+) 1221 WP_015005194.1 type II secretion system F family protein Machinery gene
  CPC19_RS11175 (CPC19_11175) pilD 2302154..2303029 (+) 876 WP_016390930.1 A24 family peptidase Machinery gene
  CPC19_RS11180 (CPC19_11180) coaE 2303036..2303641 (+) 606 WP_096910803.1 dephospho-CoA kinase -
  CPC19_RS11185 (CPC19_11185) zapD 2303682..2304449 (+) 768 WP_020933068.1 cell division protein ZapD -
  CPC19_RS11190 (CPC19_11190) yacG 2304451..2304639 (+) 189 WP_016390933.1 DNA gyrase inhibitor YacG -
  CPC19_RS11195 (CPC19_11195) - 2304663..2305598 (-) 936 WP_016390934.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63016.56 Da        Isoelectric Point: 4.8960

>NTDB_id=248995 CPC19_RS11165 WP_016390929.1 2299189..2300898(+) (pilB) [Cycloclasticus sp. PY97N]
MAVSGEKFYLGGLARRLVLDGLITEDVALKYSEEAYKKKRPFVSFLVEKKVLDSQIIALSASEEFGVPLFDITALSKDVI
PKGIVDEKLVRKHHALPIFKRGNKLFVAVSDPTNLQALDEIKFQTRMSTEAILVEEDKLARVIDTAMDDEDTSMSDLLDD
DLENINFDDPDAEPVDDLDSEIDDAPVVRFVNKILLDAIKKGASDIHFEPYEKSFRIRTRQDGILKEIASPPINISNKLI
SRIKVMSRMNISERRIPQDGRIKMALSKKRAIDFRVNTCPTLYGEKIVLRILDPTSAQVGIEQLGFDETQKEAFLTAIKK
PYGMILVTGPTGSGKTVSLYTALNLLNDPNINISTAEDPVEITVSGINQVNVNPKTGLTFAEALRAFLRQDPDIIMVGEI
RDLETAEISVKAAQTGHLVLSTLHTNDAPQTLNRLNQMGVPPYNIAASVLLIMAQRLARKLCDKCKKEETLPTELLIKEG
FSPEEAEGLTTYKAVGCDHCTNGYKGRIGIFQVMPVSEAMENLIIKGGLTTELEEQAAQEGVLTLRESGFKKVIAGITSI
EEINRITKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=248995 CPC19_RS11165 WP_016390929.1 2299189..2300898(+) (pilB) [Cycloclasticus sp. PY97N]
ATGGCTGTATCCGGCGAAAAATTTTATTTAGGTGGCTTAGCAAGACGATTGGTCTTAGACGGTCTAATTACTGAAGACGT
TGCACTCAAATATTCTGAGGAGGCTTATAAAAAGAAAAGACCTTTTGTTAGCTTTCTTGTAGAGAAAAAAGTTTTAGATA
GTCAAATTATTGCTCTTTCTGCTTCTGAAGAGTTTGGCGTTCCATTATTTGATATTACAGCACTGAGCAAGGACGTTATT
CCCAAAGGTATTGTTGACGAAAAATTAGTTCGTAAACACCATGCATTACCGATATTTAAACGTGGCAATAAGCTGTTTGT
TGCCGTATCTGACCCGACTAACCTTCAAGCGCTTGATGAAATAAAGTTCCAAACAAGGATGAGCACCGAAGCCATTCTTG
TTGAAGAGGATAAGTTAGCACGTGTTATTGACACCGCCATGGATGATGAAGATACATCCATGAGTGACTTATTGGATGAC
GACCTTGAAAATATCAATTTCGATGACCCCGATGCAGAACCGGTTGACGATCTTGACAGTGAAATTGATGACGCACCCGT
TGTGCGATTTGTTAATAAAATCTTATTGGATGCCATTAAAAAAGGTGCATCAGACATTCATTTTGAACCGTATGAGAAAA
GTTTTAGAATCCGAACTCGGCAAGATGGTATTTTAAAAGAAATTGCTTCGCCACCAATTAATATCTCAAACAAACTGATA
TCACGAATTAAGGTGATGTCGCGGATGAATATTTCAGAACGCCGTATTCCACAGGACGGTCGTATTAAAATGGCTTTATC
GAAAAAAAGGGCCATCGACTTCCGTGTTAATACTTGCCCTACTCTATATGGTGAAAAAATCGTGCTGCGTATTTTAGACC
CAACCAGCGCACAAGTCGGTATTGAGCAACTTGGGTTTGACGAAACTCAAAAGGAAGCTTTTCTAACCGCTATCAAAAAG
CCATACGGTATGATTTTGGTGACAGGACCTACCGGTAGTGGTAAGACCGTTAGCCTTTATACCGCGCTTAACTTATTAAA
CGACCCAAACATCAACATTTCAACAGCTGAAGACCCGGTTGAAATTACTGTATCGGGTATTAACCAAGTCAACGTAAACC
CAAAAACTGGCTTAACCTTTGCTGAAGCGCTTCGAGCTTTTTTACGCCAAGATCCAGATATAATCATGGTCGGCGAGATT
CGAGACCTTGAAACCGCTGAAATTTCTGTTAAAGCCGCACAAACCGGTCACCTTGTTCTATCTACATTACACACAAACGA
TGCCCCACAAACCTTAAACCGTTTAAACCAAATGGGCGTTCCACCCTACAATATTGCAGCATCTGTGTTATTAATTATGG
CGCAACGTTTAGCAAGAAAACTATGCGATAAATGTAAAAAAGAAGAGACTTTACCAACAGAGTTATTAATCAAAGAAGGC
TTTAGCCCTGAAGAAGCTGAAGGATTAACAACTTACAAGGCTGTAGGCTGTGATCATTGCACTAACGGCTATAAGGGCCG
TATTGGCATTTTCCAGGTAATGCCTGTTTCTGAAGCCATGGAAAACCTTATCATTAAAGGTGGTTTAACAACTGAACTTG
AAGAACAAGCTGCACAAGAGGGCGTTTTAACACTCAGGGAGTCTGGCTTTAAAAAGGTCATCGCTGGTATTACCAGCATA
GAAGAAATAAACCGAATCACTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.304

100

0.559
  pilB Acinetobacter baylyi ADP1

50.26

100

0.51
  pilB Acinetobacter baumannii D1279779

51.693

98.594

0.51
  pilF Neisseria gonorrhoeae MS11

48.148

99.649

0.48
  pilB Vibrio cholerae strain A1552

50.095

92.619

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

48.289

92.443

0.446
  pilB Vibrio campbellii strain DS40M4

51.346

84.886

0.436
  pilF Thermus thermophilus HB27

39.227

95.431

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.037

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

92.443

0.36

Multiple sequence alignment