Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CN538_RS19280 Genome accession   NZ_CP023559
Coordinates   4201772..4203088 (+) Length   438 a.a.
NCBI ID   WP_340638195.1    Uniprot ID   -
Organism   Salinicola tamaricis strain F01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4196772..4208088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN538_RS19260 - 4197509..4198711 (+) 1203 WP_106420470.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  CN538_RS19265 - 4198887..4200367 (-) 1481 Protein_4274 TrkH family potassium uptake protein -
  CN538_RS19270 - 4200591..4200875 (+) 285 WP_106420471.1 hypothetical protein -
  CN538_RS19275 - 4201021..4201521 (-) 501 WP_106420472.1 pilin -
  CN538_RS19280 pilB 4201772..4203088 (+) 1317 WP_340638195.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CN538_RS25715 pilB 4203103..4203501 (+) 399 WP_340638196.1 hypothetical protein Machinery gene
  CN538_RS19285 pilC 4203694..4204812 (+) 1119 WP_242495401.1 type II secretion system F family protein Machinery gene
  CN538_RS19290 pilD 4204898..4205758 (+) 861 WP_242495402.1 A24 family peptidase Machinery gene
  CN538_RS19295 coaE 4205755..4206420 (+) 666 WP_106420473.1 dephospho-CoA kinase -
  CN538_RS19300 yacG 4206417..4206659 (+) 243 WP_106420474.1 DNA gyrase inhibitor YacG -
  CN538_RS19305 - 4206750..4207703 (-) 954 WP_106420475.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 438 a.a.        Molecular weight: 48393.33 Da        Isoelectric Point: 5.2573

>NTDB_id=248396 CN538_RS19280 WP_340638195.1 4201772..4203088(+) (pilB) [Salinicola tamaricis strain F01]
MPTTSSPGSHSGIARRLIDRGLIEPAACERALHAARDAEQTLLEFLVRQGRVDPRQAGEAAAHEYGLPFLDLQHVVWDRL
PIIEPIAEKLISRFQVLPLARDDRRLTVAIAFPATLARLDELQFTTGLAIDGVLVPFDQLERALARRLQHQDTSGMLEAL
DEASDAVTLDTSEIDLEEEAAALDAGGDNDAPIVRFVNRVLRDAIQRGASDVHFEPYESSFRVRFRIDGMLLEVTRPPLN
LRTRIAARLKVMARLDISERRLPQDGAMKLRLEDQRAIDFRVNSLPTVFGEKIVLRLLDARATGWGIDALGFSAPQRELF
ESALARPQGMILVTGPTGSGKTVTLYTGLNRLNTEERNISTAEDPVELKIPGINQVNVQTRIGLDFASALRAFLRQDPDV
VMVGEIRDLETAEIAVKAAQTGHLVLSTPCTPTLPPRR

Nucleotide


Download         Length: 1317 bp        

>NTDB_id=248396 CN538_RS19280 WP_340638195.1 4201772..4203088(+) (pilB) [Salinicola tamaricis strain F01]
GTGCCGACCACCTCCTCCCCTGGCAGCCACAGCGGTATCGCCCGCCGTCTGATCGACCGCGGTCTGATCGAGCCAGCTGC
CTGCGAGCGCGCACTGCACGCGGCGAGAGACGCCGAGCAGACGCTGCTCGAGTTCCTGGTACGCCAAGGTCGGGTCGACC
CGCGTCAGGCCGGTGAGGCGGCAGCGCACGAGTACGGGCTGCCGTTTCTCGATCTGCAGCACGTGGTGTGGGACCGCCTG
CCGATCATCGAACCGATCGCCGAGAAATTGATCAGCCGTTTCCAGGTGCTGCCGCTGGCGCGTGACGACCGCCGACTGAC
AGTGGCGATCGCCTTCCCCGCCACCCTGGCCAGGCTCGATGAGTTGCAATTCACCACTGGCCTTGCGATCGACGGCGTGC
TGGTGCCGTTCGACCAGCTCGAGCGCGCCCTGGCCCGCCGCCTGCAGCACCAGGACACTTCCGGCATGCTCGAAGCGCTG
GATGAAGCCAGCGATGCGGTGACGCTGGATACCAGCGAGATCGACCTGGAAGAGGAGGCCGCGGCCCTGGATGCCGGTGG
CGACAATGACGCGCCGATCGTTCGTTTCGTCAACCGGGTGCTGCGTGATGCGATCCAGCGCGGTGCCTCGGACGTGCACT
TCGAACCCTACGAGTCGAGCTTTCGGGTGCGTTTTCGGATCGACGGCATGCTGCTGGAGGTGACCCGCCCGCCGCTCAAC
CTGCGCACCCGCATCGCCGCCCGCCTCAAGGTGATGGCACGCCTGGACATCTCCGAGCGCCGCCTGCCCCAGGACGGCGC
GATGAAACTGCGTCTGGAGGATCAGCGCGCCATCGACTTCCGCGTCAACAGCCTGCCCACGGTATTCGGCGAGAAGATCG
TGCTGCGCCTGCTCGATGCCCGCGCCACCGGATGGGGCATCGATGCGCTGGGCTTTTCAGCGCCCCAGCGCGAGCTGTTC
GAAAGCGCCCTGGCGCGCCCCCAGGGGATGATTCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGACTCTCTACAC
CGGGCTCAATCGGCTCAACACCGAAGAGCGCAACATCTCCACCGCCGAAGATCCGGTGGAACTCAAGATACCGGGGATCA
ACCAGGTCAACGTGCAGACACGCATCGGGCTCGATTTCGCCAGTGCCCTGCGCGCCTTTCTGCGCCAGGACCCGGACGTG
GTGATGGTCGGCGAGATTCGCGATCTGGAGACCGCCGAGATCGCGGTCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTC
CACCCCCTGCACACCAACTCTGCCGCCGAGACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-428)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.35

97.945

0.493
  pilB Acinetobacter baylyi ADP1

48.394

99.543

0.482
  pilB Legionella pneumophila strain ERS1305867

52.25

91.324

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

47.032

100

0.47
  pilB Vibrio campbellii strain DS40M4

48.544

94.064

0.457
  pilB Vibrio cholerae strain A1552

51.316

86.758

0.445
  pilF Neisseria gonorrhoeae MS11

43.357

97.945

0.425
  pilF Thermus thermophilus HB27

37.915

96.347

0.365

Multiple sequence alignment