Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC72_RS01770 Genome accession   NZ_CP023472
Coordinates   338043..339791 (+) Length   582 a.a.
NCBI ID   WP_162935422.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_072     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333043..344791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC72_RS01750 (FORC72_0272) pilM 335342..336358 (+) 1017 WP_031821574.1 type IV pilus assembly protein PilM Machinery gene
  FORC72_RS01755 (FORC72_0273) pilN 336342..336920 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  FORC72_RS01760 (FORC72_0274) pilO 336913..337506 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC72_RS01765 (FORC72_0275) pilP 337496..338011 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  FORC72_RS01770 (FORC72_0276) pilQ 338043..339791 (+) 1749 WP_162935422.1 type IV pilus secretin PilQ family protein Machinery gene
  FORC72_RS01775 (FORC72_0277) aroK 339980..340498 (+) 519 WP_005381319.1 shikimate kinase AroK -
  FORC72_RS01780 (FORC72_0278) aroB 340530..341630 (+) 1101 WP_120458557.1 3-dehydroquinate synthase -
  FORC72_RS01785 (FORC72_0279) - 341683..343200 (+) 1518 WP_029851669.1 AAA family ATPase -
  FORC72_RS01790 (FORC72_0280) - 343270..344109 (+) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63853.00 Da        Isoelectric Point: 4.4411

>NTDB_id=247326 FORC72_RS01770 WP_162935422.1 338043..339791(+) (pilQ) [Vibrio parahaemolyticus strain FORC_072]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDISTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEDNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGSDGEGEEGGDGVGIDDFLNVNLAATNKSA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=247326 FORC72_RS01770 WP_162935422.1 338043..339791(+) (pilQ) [Vibrio parahaemolyticus strain FORC_072]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAGCAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATATCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGATTCAGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAACAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCAACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGTGAGCTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATTAATGGTGAAGACAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTAAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAATGAGGGGAACATGGATGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTTGCGACAATCGGCTTGTACGAAGGTGGTAGTGACG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTCCTTAATGTGAACTTAGCTGCAACTAATAAAAGTGCT
GCAAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTACAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCTGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGGCCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGCACCCAAGTTCTGGTTAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTCCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGTAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.065

100

0.911
  pilQ Vibrio cholerae strain A1552

76.232

97.595

0.744
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.232

97.595

0.744
  pilQ Pseudomonas aeruginosa PAK

39.049

100

0.395
  pilQ Legionella pneumophila strain Lp02

41.273

94.502

0.39
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.502

0.39
  pilQ Acinetobacter baumannii strain A118

37.291

100

0.383
  pilQ Acinetobacter baumannii D1279779

37.291

100

0.383
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment