Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC72_RS01750 Genome accession   NZ_CP023472
Coordinates   335342..336358 (+) Length   338 a.a.
NCBI ID   WP_031821574.1    Uniprot ID   A0AA47L644
Organism   Vibrio parahaemolyticus strain FORC_072     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330342..341358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC72_RS01735 (FORC72_0269) - 330766..331494 (-) 729 WP_015297397.1 redoxin family protein -
  FORC72_RS01740 (FORC72_0270) oxyR 331665..332573 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC72_RS01745 (FORC72_0271) - 332629..335199 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  FORC72_RS01750 (FORC72_0272) pilM 335342..336358 (+) 1017 WP_031821574.1 type IV pilus assembly protein PilM Machinery gene
  FORC72_RS01755 (FORC72_0273) pilN 336342..336920 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  FORC72_RS01760 (FORC72_0274) pilO 336913..337506 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC72_RS01765 (FORC72_0275) pilP 337496..338011 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  FORC72_RS01770 (FORC72_0276) pilQ 338043..339791 (+) 1749 WP_162935422.1 type IV pilus secretin PilQ family protein Machinery gene
  FORC72_RS01775 (FORC72_0277) aroK 339980..340498 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37720.16 Da        Isoelectric Point: 6.6631

>NTDB_id=247322 FORC72_RS01750 WP_031821574.1 335342..336358(+) (pilM) [Vibrio parahaemolyticus strain FORC_072]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWNLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=247322 FORC72_RS01750 WP_031821574.1 335342..336358(+) (pilM) [Vibrio parahaemolyticus strain FORC_072]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAATCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCAT
GCGGTACTCGTATTTCAATGGGAACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.32

100

0.843
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment