Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CNR27_RS05335 Genome accession   NZ_CP023406
Coordinates   1172385..1174109 (-) Length   574 a.a.
NCBI ID   WP_096297261.1    Uniprot ID   A0A290XCR1
Organism   Luteimonas chenhongjianii strain 100111     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1167385..1179109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNR27_RS05315 (CNR27_05315) - 1168068..1169015 (+) 948 WP_096297257.1 Nudix family hydrolase -
  CNR27_RS05320 (CNR27_05320) coaE 1169442..1170089 (-) 648 WP_096297258.1 dephospho-CoA kinase -
  CNR27_RS05325 (CNR27_05325) pilD 1170102..1170965 (-) 864 WP_096297259.1 A24 family peptidase Machinery gene
  CNR27_RS05330 (CNR27_05330) pilC 1170979..1172256 (-) 1278 WP_096297260.1 type II secretion system F family protein Machinery gene
  CNR27_RS05335 (CNR27_05335) pilB 1172385..1174109 (-) 1725 WP_096297261.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CNR27_RS05340 (CNR27_05340) - 1174439..1174906 (-) 468 WP_096297262.1 pilin -
  CNR27_RS05345 (CNR27_05345) - 1175106..1175825 (-) 720 WP_096297263.1 hypothetical protein -
  CNR27_RS05350 (CNR27_05350) pilR 1176009..1177439 (-) 1431 WP_096297264.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62895.04 Da        Isoelectric Point: 5.7749

>NTDB_id=246732 CNR27_RS05335 WP_096297261.1 1172385..1174109(-) (pilB) [Luteimonas chenhongjianii strain 100111]
MNAVSSQNLVGITGIARRLVLDGALDEIAAREALDKATAARKQISQYLREHKLVTAAQMAAANSIEFGMPIFDPSAMDSQ
ASAIKLVSEELLQKHAVLPLFKRGPRLYVGIADPTDAQALDEIKFHTNLTVEPILVDEDSIRRTQDIWLQSSDNFGDSLD
DSDGLDALDVGSDDDAGKIENVADGKDDTPIVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQAAKMPVKLN
GRITARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIDKLGYEPDQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGKCKRRVDLPEHAL
LAEGFNEDQVRAGIELYEPAGCDECTNGYKGRVGVYQVMPMSEQIQEIILQGGNAIQIAEVAERAGVNDLRKSALEKARQ
GLTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=246732 CNR27_RS05335 WP_096297261.1 1172385..1174109(-) (pilB) [Luteimonas chenhongjianii strain 100111]
ATGAACGCTGTTTCCTCACAGAACCTCGTCGGCATCACCGGCATTGCCCGCCGTCTCGTGCTGGACGGCGCGCTCGACGA
AATCGCAGCGCGAGAAGCACTCGACAAAGCCACCGCCGCCCGCAAGCAGATCTCGCAGTACCTGCGCGAACACAAGCTGG
TCACGGCCGCGCAGATGGCCGCGGCCAATTCGATCGAGTTCGGAATGCCGATCTTCGATCCGTCGGCGATGGACAGCCAG
GCCAGCGCGATCAAGCTGGTCAGCGAGGAACTGCTGCAGAAGCACGCGGTGCTGCCGTTGTTCAAGCGTGGACCGCGCCT
CTATGTCGGCATCGCGGATCCGACCGACGCGCAGGCGCTGGACGAGATCAAGTTCCACACGAACCTGACCGTCGAGCCGA
TCCTGGTCGACGAGGACAGCATCCGCCGCACCCAGGACATCTGGCTGCAATCGTCCGACAATTTCGGCGACTCCCTGGAC
GACTCCGACGGCCTCGATGCGCTCGACGTGGGCAGCGATGACGATGCCGGCAAGATCGAGAACGTGGCGGACGGCAAGGA
CGACACGCCGATCGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCAAGCGCGGCGCCTCCGACATCCACTTCGAGC
CCTACGAGACCGATTATCGGGTGCGCCTGCGCATCGACGGCATTCTCAAGCAGGCGGCGAAGATGCCGGTCAAGCTCAAC
GGACGCATTACCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCGCATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGGTGGTGCTGC
GTATCCTCGATGCCAGTGCGGCGAAGCTGGGCATCGACAAGCTGGGTTACGAGCCCGACCAGCAGAAACTGTTCCTCGAC
GCCATCCATAAACCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATACAGCGCT
CGGCATCCTCAACGACGAGACCCGCAATATCTCGACCGCGGAGGACCCGGTGGAAATCCGCCTGCCCGGCGTGAACCAGG
TGCAGCAGAACAACAAGCGCGGCATGACATTTGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAGATCCGAGATCTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTCTCCACGCT
GCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGGATCGCGCCGTACAACATCACCAGCTCGGTGA
CCCTGGTCATCGCCCAGCGCCTGGCGCGCCGGCTCTGCGGCAAGTGCAAGCGCCGCGTCGACCTGCCCGAGCACGCGCTG
CTGGCCGAAGGATTCAACGAAGACCAGGTGCGTGCCGGCATTGAACTGTACGAACCCGCGGGCTGCGACGAGTGCACCAA
TGGCTACAAGGGTCGCGTCGGCGTGTACCAGGTGATGCCGATGAGCGAGCAGATCCAGGAGATCATCCTGCAGGGCGGCA
ACGCGATCCAGATCGCCGAAGTGGCCGAGCGCGCGGGCGTCAACGACCTGCGCAAGTCCGCGCTGGAGAAAGCGCGCCAG
GGCCTGACCAGCCTGGCCGAAATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-145)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A290XCR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.379

98.78

0.547
  pilB Acinetobacter baylyi ADP1

55.026

98.78

0.544
  pilB Legionella pneumophila strain ERS1305867

52.473

98.606

0.517
  pilB Vibrio cholerae strain A1552

49.912

99.477

0.497
  pilF Neisseria gonorrhoeae MS11

47.5

97.561

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

49.237

91.289

0.449
  pilB Vibrio campbellii strain DS40M4

51.434

85.017

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

37.739

100

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.349

94.948

0.364
  pilF Thermus thermophilus HB27

37.705

95.645

0.361

Multiple sequence alignment