Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   CMV25_RS06240 Genome accession   NZ_CP023392
Coordinates   1243643..1244038 (+) Length   131 a.a.
NCBI ID   WP_096039919.1    Uniprot ID   A0AAE6YLF4
Organism   Lactococcus raffinolactis strain WiKim0068     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1212148..1250286 1243643..1244038 within 0


Gene organization within MGE regions


Location: 1212148..1250286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV25_RS06090 (CMV25_06090) - 1212148..1213290 (+) 1143 WP_096039892.1 beta 1-4 rhamnosyltransferase Cps2T -
  CMV25_RS06095 (CMV25_06095) - 1213280..1214215 (+) 936 WP_096039893.1 glycosyltransferase family 2 protein -
  CMV25_RS06100 (CMV25_06100) - 1214212..1215009 (+) 798 WP_096039894.1 ABC transporter permease -
  CMV25_RS06105 (CMV25_06105) - 1215011..1216219 (+) 1209 WP_096039895.1 ABC transporter ATP-binding protein -
  CMV25_RS06110 (CMV25_06110) - 1216346..1217320 (+) 975 WP_096039896.1 glycosyltransferase family A protein -
  CMV25_RS06115 (CMV25_06115) - 1217329..1218579 (+) 1251 WP_096039897.1 glycosyl transferase -
  CMV25_RS06120 (CMV25_06120) - 1218580..1219536 (+) 957 WP_096039898.1 NAD-dependent epimerase/dehydratase family protein -
  CMV25_RS06125 (CMV25_06125) - 1219582..1220562 (+) 981 WP_096039899.1 glycosyltransferase family 2 protein -
  CMV25_RS06130 (CMV25_06130) - 1220604..1221992 (+) 1389 WP_157738507.1 DUF2142 domain-containing protein -
  CMV25_RS06135 (CMV25_06135) - 1221992..1222507 (+) 516 Protein_1196 hypothetical protein -
  CMV25_RS06140 (CMV25_06140) - 1222555..1223280 (+) 726 WP_096039901.1 glycosyltransferase family 2 protein -
  CMV25_RS06145 (CMV25_06145) - 1223285..1223653 (+) 369 WP_167372343.1 DUF2304 domain-containing protein -
  CMV25_RS06150 (CMV25_06150) - 1223705..1224547 (+) 843 WP_096039903.1 NAD(P)-dependent oxidoreductase -
  CMV25_RS06155 (CMV25_06155) - 1224537..1225334 (+) 798 WP_096039904.1 glycosyl transferase -
  CMV25_RS06160 (CMV25_06160) - 1225345..1226364 (+) 1020 WP_096039905.1 acyltransferase -
  CMV25_RS06165 (CMV25_06165) - 1226368..1227381 (+) 1014 WP_096039906.1 capsular polysaccharide synthesis protein -
  CMV25_RS06170 (CMV25_06170) - 1227400..1228227 (+) 828 WP_096039907.1 phosphorylcholine transferase LicD -
  CMV25_RS06175 (CMV25_06175) - 1228229..1228960 (+) 732 WP_096039908.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CMV25_RS06180 (CMV25_06180) - 1228954..1230012 (+) 1059 WP_208616179.1 NAD-dependent epimerase/dehydratase family protein -
  CMV25_RS06185 (CMV25_06185) - 1230014..1230952 (+) 939 WP_096039910.1 glycosyltransferase -
  CMV25_RS06190 (CMV25_06190) - 1231012..1232538 (+) 1527 WP_096039911.1 lipopolysaccharide biosynthesis protein -
  CMV25_RS06195 (CMV25_06195) - 1232759..1234585 (+) 1827 WP_096039912.1 glycosyltransferase family 39 protein -
  CMV25_RS06200 (CMV25_06200) - 1234582..1235517 (+) 936 WP_096039913.1 glycosyltransferase family 2 protein -
  CMV25_RS06205 (CMV25_06205) - 1235685..1237616 (+) 1932 WP_096039914.1 Ig-like domain-containing protein -
  CMV25_RS06210 (CMV25_06210) - 1237684..1238040 (+) 357 WP_096039915.1 hypothetical protein -
  CMV25_RS11700 (CMV25_06215) - 1238095..1241019 (+) 2925 WP_096039916.1 glucosaminidase domain-containing protein -
  CMV25_RS06220 (CMV25_06220) pepA 1241073..1242158 (-) 1086 WP_096039917.1 glutamyl aminopeptidase -
  CMV25_RS06225 (CMV25_06225) - 1242297..1242596 (+) 300 WP_061773650.1 hypothetical protein -
  CMV25_RS06230 (CMV25_06230) - 1242609..1242947 (+) 339 WP_096039918.1 thioredoxin family protein -
  CMV25_RS06235 (CMV25_06235) ytpR 1242956..1243579 (+) 624 WP_061773652.1 YtpR family tRNA-binding protein -
  CMV25_RS06240 (CMV25_06240) ssbA 1243643..1244038 (+) 396 WP_096039919.1 single-stranded DNA-binding protein Machinery gene
  CMV25_RS11575 - 1244072..1244377 (+) 306 WP_208616161.1 hypothetical protein -
  CMV25_RS11580 - 1244381..1244605 (+) 225 WP_208616162.1 CHAP domain-containing protein -
  CMV25_RS11585 - 1244668..1245231 (+) 564 WP_208616163.1 hypothetical protein -
  CMV25_RS06250 (CMV25_06250) groES 1245402..1245686 (+) 285 WP_061773656.1 co-chaperone GroES -
  CMV25_RS06255 (CMV25_06255) groL 1245754..1247385 (+) 1632 WP_096039920.1 chaperonin GroEL -
  CMV25_RS06260 (CMV25_06260) - 1247588..1247848 (+) 261 WP_096039921.1 SemiSWEET family transporter -
  CMV25_RS06265 (CMV25_06265) - 1247993..1248511 (-) 519 WP_061773659.1 hypothetical protein -
  CMV25_RS06270 (CMV25_06270) - 1248994..1250286 (+) 1293 WP_096039922.1 adenylosuccinate synthase -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14707.89 Da        Isoelectric Point: 7.1673

>NTDB_id=246603 CMV25_RS06240 WP_096039919.1 1243643..1244038(+) (ssbA) [Lactococcus raffinolactis strain WiKim0068]
MLNKSLLIGRLTAQPEVKKTPTDKSVLRVTLAVSRQFKNADGQRDADFLTIIIWGKPAELFASYGKKGSLVSIEGELRTR
RYEDKAGVTHYVTEVLCHQFNLLESRAAVALRENQVETVTDVMLEAEDLPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=246603 CMV25_RS06240 WP_096039919.1 1243643..1244038(+) (ssbA) [Lactococcus raffinolactis strain WiKim0068]
ATGTTAAATAAAAGTTTACTAATTGGTCGTTTGACCGCACAGCCGGAAGTGAAGAAAACACCAACGGATAAATCTGTCTT
GCGCGTGACCTTAGCTGTTAGCCGACAATTTAAAAACGCTGATGGGCAACGGGATGCAGATTTTTTGACCATTATTATTT
GGGGCAAACCCGCAGAGCTATTTGCATCCTACGGTAAAAAAGGCAGTCTAGTTTCGATTGAGGGAGAACTCCGAACGCGT
CGTTATGAGGACAAAGCTGGTGTAACTCACTATGTGACAGAAGTCCTATGTCATCAGTTCAATCTGTTAGAAAGTCGAGC
AGCAGTTGCGCTTAGAGAGAATCAGGTTGAGACGGTGACCGATGTCATGCTAGAGGCTGAAGATTTGCCGTTTTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Streptococcus mutans UA159

62.879

100

0.634
  ssbB Streptococcus sobrinus strain NIDR 6715-7

60.606

100

0.611
  ssbB/cilA Streptococcus pneumoniae TIGR4

56.061

100

0.565
  ssbB/cilA Streptococcus mitis NCTC 12261

56.061

100

0.565
  ssbB/cilA Streptococcus pneumoniae Rx1

55.303

100

0.557
  ssbB/cilA Streptococcus pneumoniae R6

55.303

100

0.557
  ssbB/cilA Streptococcus mitis SK321

55.303

100

0.557
  ssbB/cilA Streptococcus pneumoniae D39

55.303

100

0.557
  ssbB Lactococcus lactis subsp. cremoris KW2

59.829

89.313

0.534
  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405

Multiple sequence alignment