Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   CMV25_RS04345 Genome accession   NZ_CP023392
Coordinates   875132..876073 (-) Length   313 a.a.
NCBI ID   WP_061774317.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain WiKim0068     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870132..881073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV25_RS04300 (CMV25_04300) - 870132..870581 (-) 450 WP_096039675.1 zinc-dependent MarR family transcriptional regulator -
  CMV25_RS04305 (CMV25_04305) - 870657..871505 (-) 849 WP_096039676.1 aminotransferase class IV -
  CMV25_RS04310 (CMV25_04310) ispE 871589..872440 (-) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  CMV25_RS04315 (CMV25_04315) comGG 872445..872729 (-) 285 WP_096039677.1 competence type IV pilus minor pilin ComGG -
  CMV25_RS04320 (CMV25_04320) comGF 872741..873184 (-) 444 WP_244165991.1 competence type IV pilus minor pilin ComGF -
  CMV25_RS04325 (CMV25_04325) comGE 873159..873464 (-) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  CMV25_RS11735 (CMV25_04330) comGD 873436..873921 (-) 486 WP_341865229.1 competence type IV pilus minor pilin ComGD -
  CMV25_RS04335 (CMV25_04335) comYC 873836..874156 (-) 321 WP_061774318.1 competence type IV pilus major pilin ComGC Machinery gene
  CMV25_RS04340 (CMV25_04340) comYB 874165..875250 (-) 1086 WP_310592697.1 competence type IV pilus assembly protein ComGB Machinery gene
  CMV25_RS04345 (CMV25_04345) comGA/cglA/cilD 875132..876073 (-) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  CMV25_RS04350 (CMV25_04350) - 876136..877980 (-) 1845 WP_096039681.1 acyltransferase family protein -
  CMV25_RS04355 (CMV25_04355) - 877977..878339 (-) 363 WP_096039682.1 hypothetical protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34933.21 Da        Isoelectric Point: 9.1747

>NTDB_id=246598 CMV25_RS04345 WP_061774317.1 875132..876073(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain WiKim0068]
MIKEIAQRMLKKASDFGASDIYILPARTGFSVVFRKSAHREYDQLLSDAEGQSLISHFKFTAGMNVGEKRRPQLGSCLYE
LADRKCRLRLSSAGDFESRESLVIRILHDTKQPLKFWIEADLPQVKKLVARRGLYLFAGPVGSGKTTLMHHIAKEKFSGQ
QVITIEDPVEIVAPDLLQFQLNEAIGNTYDSLIKLSLRHMPDLVIVGEIRDQETARAVMRASLTGYTVFSTIHAKSIAGV
YARLLELGVTKEEINNSLSGVVYQRLIAGKGVLDSAEKAFERHANDKWHAKIEKLVTEGHLTPDQATAEKVSD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=246598 CMV25_RS04345 WP_061774317.1 875132..876073(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain WiKim0068]
ATGATAAAGGAAATAGCGCAAAGGATGTTAAAGAAAGCAAGCGATTTTGGGGCAAGTGATATTTATATATTACCCGCCAG
AACAGGATTTTCGGTTGTTTTCAGAAAGTCAGCACATCGTGAATATGATCAGCTCCTGTCAGATGCTGAAGGTCAAAGTC
TCATTTCCCATTTCAAATTTACAGCAGGGATGAATGTAGGTGAGAAGCGCCGGCCACAACTAGGATCTTGCTTGTATGAG
CTTGCAGATAGAAAGTGTCGCTTGCGTCTATCCTCAGCGGGTGACTTTGAAAGTCGCGAAAGTTTGGTGATTCGAATTTT
GCATGACACGAAACAACCACTCAAGTTTTGGATTGAGGCAGATTTACCTCAGGTAAAAAAGTTAGTAGCTAGACGCGGTC
TTTATTTATTTGCGGGGCCAGTTGGTTCTGGGAAAACGACGCTGATGCATCACATTGCCAAAGAAAAGTTTTCTGGTCAG
CAAGTCATTACGATTGAAGATCCAGTTGAAATCGTTGCACCAGATCTGCTCCAATTCCAACTGAATGAAGCGATTGGCAA
CACCTATGATAGCTTGATTAAATTATCCTTGCGCCATATGCCAGATCTTGTGATTGTCGGAGAAATTCGAGATCAGGAAA
CTGCGCGAGCAGTCATGAGGGCCAGTCTGACGGGCTATACTGTTTTTTCGACGATTCATGCTAAGTCGATTGCGGGTGTT
TATGCGCGATTATTGGAGCTTGGCGTAACAAAAGAAGAAATCAATAATTCGTTGTCAGGTGTTGTCTATCAACGGTTGAT
TGCAGGAAAGGGGGTGCTAGATAGTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAA
AATTGGTTACAGAAGGACATCTCACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae R6

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae D39

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

56.731

99.681

0.565
  comGA Lactococcus lactis subsp. cremoris KW2

56.23

100

0.562
  comYA Streptococcus gordonii str. Challis substr. CH1

55.911

100

0.559
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

53.674

100

0.537
  comYA Streptococcus mutans UA140

52.548

100

0.527
  comYA Streptococcus mutans UA159

52.548

100

0.527

Multiple sequence alignment