Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSYR_RS04130 Genome accession   NC_007005
Coordinates   902289..903506 (-) Length   405 a.a.
NCBI ID   WP_011266635.1    Uniprot ID   A0AAD0IB66
Organism   Pseudomonas syringae pv. syringae B728a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897289..908506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYR_RS04100 (Psyr_0791) - 898715..899248 (-) 534 WP_003406554.1 MOSC domain-containing protein -
  PSYR_RS04105 (Psyr_0792) - 899245..899874 (-) 630 WP_011266631.1 DUF1780 domain-containing protein -
  PSYR_RS04110 (Psyr_0793) - 900001..900462 (+) 462 WP_024960617.1 hypothetical protein -
  PSYR_RS04115 (Psyr_0794) yacG 900589..900798 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYR_RS04120 (Psyr_0795) coaE 900795..901418 (-) 624 WP_011266633.1 dephospho-CoA kinase -
  PSYR_RS04125 (Psyr_0796) pilD 901415..902287 (-) 873 WP_011266634.1 prepilin peptidase Machinery gene
  PSYR_RS04130 (Psyr_0797) pilC 902289..903506 (-) 1218 WP_011266635.1 type II secretion system F family protein Machinery gene
  PSYR_RS04135 (Psyr_0798) pilB 903509..905203 (-) 1695 WP_011266636.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYR_RS04140 (Psyr_0799) - 905429..905848 (+) 420 WP_011266637.1 pilin -
  PSYR_RS04145 (Psyr_0800) - 906023..906568 (-) 546 WP_010415546.1 DUF1523 family protein -
  PSYR_RS04150 (Psyr_0801) - 906688..907686 (+) 999 WP_011266638.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44124.10 Da        Isoelectric Point: 10.1956

>NTDB_id=24324 PSYR_RS04130 WP_011266635.1 902289..903506(-) (pilC) [Pseudomonas syringae pv. syringae B728a]
MASKAAKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVALIVSGILLIKVVPQFQSVFASFGAQLPTFTLMVIGLSDVVQKWWLAIVGLFFVSFFIFK
RAYKQSQKFRDSLDRLLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVIKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=24324 PSYR_RS04130 WP_011266635.1 902289..903506(-) (pilC) [Pseudomonas syringae pv. syringae B728a]
ATGGCCAGCAAGGCAGCAAAAGTCATCGTTTACACTTGGGAAGGTGTTGACAAGAAAGGTACTAAAACCAGTGGGGAACT
GAGTGGCCATAACTTGGCCCTTGTAAAAGCCCAGCTGCGCAAGCAAGGCATCAACCCAACCAAGGTACGCAAAAAATCAG
TTTCGATCTTTGGCAAGGGCAAGAAGATCAAACCGCTGGACATTGCGTTCTTTTCTCGCCAGATGGCTACGATGATGAAG
GCCGGTGTGCCATTGCTGCAATCGTTTGACATCATTAGCGAAGGCGCTGAGAACCCTAACATGCGCGCATTGGTTGGTTC
GCTAAAGCAGGAAGTTTCGGCAGGTAATAGTTTTGCGACGGCTCTGCGACAAAAACCCGAATATTTCGATGATCTGTTTT
GTAACCTAGTAGATGCCGGCGAGCAAGCTGGCGCACTTGAAAGCCTGCTGGACCGTGTAGCGAGCTATAAAGAGAAGACC
GAAAAGCTAAAAGCTAAAATCAAGAAAGCCATGACATACCCGATTGCCGTATTGATTGTTGCATTGATTGTATCCGGCAT
TCTGCTGATCAAAGTGGTGCCGCAGTTCCAGTCAGTCTTCGCTAGCTTTGGCGCTCAGTTACCGACGTTTACCCTGATGG
TTATTGGTCTATCGGACGTCGTACAAAAATGGTGGCTGGCCATTGTCGGGCTATTTTTCGTCAGCTTTTTTATTTTTAAA
CGTGCTTACAAACAGTCTCAAAAGTTTCGAGATAGCCTCGACCGACTTCTGCTGAAAGTGCCCATCATTGGGCCGTTAAT
TTTCAAATCTTCGGTAGCACGCTACGCGCGCACGCTTGCAACAACGTTTGCCGCTGGCGTCCCGCTTGTGGAGGCTTTGG
ACTCAGTCGCCGGAGCGACTGGCAATGTAGTATTTAAAAATGCAGTCATCAAGGTAAAACAGGACGTCTCCACGGGTATG
CAACTGAATTTCTCGATGCGTTCAACAGGAGTGTTTCCGAGCTTGGCGATTCAGATGACAGCCATTGGCGAAGAGTCCGG
CGCGCTGGACACCATGCTCGACAAGGTAGCTACCTATTACGAGGACGAAGTCGATAACATGGTCGACAATCTGACCAGCC
TTATGGAACCGATGATCATGGCCTTTTTGGGCGTGATAGTTGGCGGGCTGGTAATCGCGATGTATCTACCTATCTTCCAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Legionella pneumophila strain ERS1305867

50.985

100

0.511
  pilC Vibrio campbellii strain DS40M4

42.998

100

0.432
  pilG Neisseria gonorrhoeae MS11

42.821

98.025

0.42
  pilC Vibrio cholerae strain A1552

42.677

97.778

0.417
  pilG Neisseria meningitidis 44/76-A

42.569

98.025

0.417
  pilC Thermus thermophilus HB27

39.401

99.012

0.39

Multiple sequence alignment