Detailed information NTG0024323

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSYR_RS04125
• Genome accession: NC_007005
• Coordinates: 901415..902287 (-)
• Length: 290 a.a.
• NCBI ID: WP_011266634.1
• Uniprot ID: Q4ZYB8
• Organism: Pseudomonas syringae pv. syringae B728a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 896415..907287

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYR_RS04095 (Psyr_0790)	-	897200..898498 (-)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-
PSYR_RS04100 (Psyr_0791)	-	898715..899248 (-)	534	WP_003406554.1	MOSC domain-containing protein	-
PSYR_RS04105 (Psyr_0792)	-	899245..899874 (-)	630	WP_011266631.1	DUF1780 domain-containing protein	-
PSYR_RS04110 (Psyr_0793)	-	900001..900462 (+)	462	WP_024960617.1	hypothetical protein	-
PSYR_RS04115 (Psyr_0794)	yacG	900589..900798 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
PSYR_RS04120 (Psyr_0795)	coaE	900795..901418 (-)	624	WP_011266633.1	dephospho-CoA kinase	-
PSYR_RS04125 (Psyr_0796)	pilD	901415..902287 (-)	873	WP_011266634.1	prepilin peptidase	Machinery gene
PSYR_RS04130 (Psyr_0797)	pilC	902289..903506 (-)	1218	WP_011266635.1	type II secretion system F family protein	Machinery gene
PSYR_RS04135 (Psyr_0798)	pilB	903509..905203 (-)	1695	WP_011266636.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSYR_RS04140 (Psyr_0799)	-	905429..905848 (+)	420	WP_011266637.1	pilin	-
PSYR_RS04145 (Psyr_0800)	-	906023..906568 (-)	546	WP_010415546.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32009.19 Da
• Isoelectric Point: 8.3734

>NTDB_id=24323 PSYR_RS04125 WP_011266634.1 901415..902287(-) (pilD) [Pseudomonas syringae pv. syringae B728a]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVIVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQATAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=24323 PSYR_RS04125 WP_011266634.1 901415..902287(-) (pilD) [Pseudomonas syringae pv. syringae B728a]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGATCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCTGCTGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCTTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
TTACCCACTGGTCGAGCTGACCTGCGCAGTGTTATCGGCATACGTGGCCTGGCATTTCGGGTTCGGCTGGCAGGCGACGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCCGATCACCAACTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAATGATGCGCTGTGGGGTGCAGT
GGCCGGGTATCTGGCACTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGGTTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: Q4ZYB8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.19	94.483	0.493
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472