Detailed information NTG0240749

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: CF389_RS16150
• Genome accession: NZ_CP022556
• Coordinates: 3267370..3268515 (-)
• Length: 381 a.a.
• NCBI ID: WP_003151067.1
• Uniprot ID: A0A6A8LBV3
• Organism: Bacillus velezensis strain NJAU-Z9
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3262370..3273515

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CF389_RS16100	speE	3262662..3263492 (+)	831	WP_003151052.1	spermidine synthase	-
CF389_RS16105	speB	3263552..3264424 (+)	873	WP_003151054.1	agmatinase	-
CF389_RS16110	-	3264594..3264812 (+)	219	WP_003151056.1	helix-turn-helix transcriptional regulator	-
CF389_RS16120	-	3265403..3265915 (-)	513	WP_003151059.1	stage II sporulation protein M	-
CF389_RS16125	-	3265959..3266240 (-)	282	WP_003151060.1	hypothetical protein	-
CF389_RS16135	-	3266648..3266851 (-)	204	WP_003151062.1	hypothetical protein	-
CF389_RS16140	-	3266865..3267068 (-)	204	WP_003151064.1	helix-turn-helix transcriptional regulator	-
CF389_RS16145	-	3267267..3267386 (-)	120	WP_003151066.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
CF389_RS16150	rapF	3267370..3268515 (-)	1146	WP_003151067.1	tetratricopeptide repeat protein	Regulator
CF389_RS16155	-	3268807..3269235 (+)	429	WP_003151069.1	DUF1934 domain-containing protein	-
CF389_RS16160	argS	3269232..3270902 (+)	1671	WP_003151071.1	arginine--tRNA ligase	-
CF389_RS16165	narK	3271037..3272227 (+)	1191	WP_003151073.1	nitrate transporter NarK	-
CF389_RS16170	-	3272325..3273041 (+)	717	WP_003151075.1	Crp/Fnr family transcriptional regulator	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45339.49 Da
• Isoelectric Point: 4.9293

>NTDB_id=240749 CF389_RS16150 WP_003151067.1 3267370..3268515(-) (rapF) [Bacillus velezensis strain NJAU-Z9]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide

• Length: 1146 bp

>NTDB_id=240749 CF389_RS16150 WP_003151067.1 3267370..3268515(-) (rapF) [Bacillus velezensis strain NJAU-Z9]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (223-287)

3D structure

• AlphaFold DB: A0A6A8LBV3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	74.803	100	0.748
rapC	Bacillus subtilis subsp. subtilis str. 168	53.158	99.738	0.53