Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PNC201_RS04235 Genome accession   NZ_CP022522
Coordinates   1044189..1045421 (-) Length   410 a.a.
NCBI ID   WP_102056264.1    Uniprot ID   A0A2K9JXJ7
Organism   Pseudoalteromonas sp. NC201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1039189..1050421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNC201_RS04215 (PNC201_04185) rlmM 1039221..1040306 (+) 1086 WP_102056261.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  PNC201_RS04220 (PNC201_04190) pilB 1040752..1042428 (-) 1677 WP_010607343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PNC201_RS04225 (PNC201_04195) coaE 1042538..1043167 (-) 630 WP_102056262.1 dephospho-CoA kinase -
  PNC201_RS04230 (PNC201_04200) pilD 1043177..1044055 (-) 879 WP_102056263.1 A24 family peptidase Machinery gene
  PNC201_RS04235 (PNC201_04205) pilC 1044189..1045421 (-) 1233 WP_102056264.1 type II secretion system F family protein Machinery gene
  PNC201_RS04240 (PNC201_04210) - 1045425..1045898 (-) 474 WP_102056265.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PNC201_RS04245 (PNC201_04215) - 1045885..1046304 (-) 420 WP_102056266.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PNC201_RS04250 (PNC201_04220) nadC 1046964..1047806 (-) 843 WP_102057843.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PNC201_RS04255 (PNC201_04225) ampD 1048512..1049054 (+) 543 WP_102056267.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PNC201_RS04260 (PNC201_04230) ampE 1049061..1049912 (+) 852 WP_095727228.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44761.71 Da        Isoelectric Point: 9.7568

>NTDB_id=240205 PNC201_RS04235 WP_102056264.1 1044189..1045421(-) (pilC) [Pseudoalteromonas sp. NC201]
MKSDKNNANNLDTFIWSGVNNRGKKLQGEMTGQSVALVKAQLRKQGVIPSKVKRKPKPLFGFGGAKKITAADIATTTRQI
ATMLMAGVSLVQTLEMLSKGIKNKSLSKLIGHICDEVKAGQPLARSLRAHPRYFDELYCDLVHSGEQSGALDTIFDRVAL
YKEKSEALKSKIKKALFYPVAVIAVALIVTGILLIFVVPQFQDIFNGFGAELPAFTLMVIAISEFMQEYWWVFVLGIGLF
GYTYKEMLIRSPAVKHFNDRMMLRIPVIGEILKKAAVARYARTLSTTFAAGVPLGDALDSAAGASGNIIYKNAIKEIKAE
VNSGNQMHWAMHNTKVFPDMVVQMVSIGEESGSLDGMLAKVANIYEQEVDDAVDGLSSLLEPLIMVVLGVLIGGLIIAMY
LPIFQLGSVI

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=240205 PNC201_RS04235 WP_102056264.1 1044189..1045421(-) (pilC) [Pseudoalteromonas sp. NC201]
ATGAAATCAGATAAGAATAATGCTAATAACTTAGACACTTTTATATGGTCCGGTGTCAATAACCGAGGAAAAAAGCTTCA
AGGTGAAATGACAGGTCAGAGTGTCGCTTTGGTGAAAGCTCAACTTCGCAAGCAAGGCGTTATTCCGTCAAAAGTAAAGC
GAAAACCCAAACCCTTATTTGGTTTTGGTGGCGCAAAGAAAATAACCGCCGCAGATATTGCGACTACCACCAGACAAATT
GCCACCATGTTGATGGCTGGTGTTTCTTTGGTACAGACCTTAGAAATGCTCTCAAAAGGAATAAAAAATAAAAGCCTATC
AAAGTTAATTGGCCACATTTGCGATGAAGTTAAAGCAGGACAACCTCTTGCTAGATCACTGCGTGCTCATCCAAGATATT
TCGATGAACTTTATTGCGATCTTGTCCACTCAGGCGAACAATCGGGAGCACTAGATACTATTTTTGATCGAGTAGCTCTA
TATAAAGAAAAATCAGAAGCACTAAAGTCCAAAATTAAAAAAGCATTATTTTATCCAGTCGCTGTTATTGCTGTAGCCCT
AATTGTTACTGGTATTTTGCTTATTTTCGTTGTACCTCAGTTTCAGGATATATTTAACGGTTTTGGTGCCGAACTCCCAG
CTTTTACGCTAATGGTTATCGCGATTTCCGAATTTATGCAGGAATATTGGTGGGTATTTGTACTTGGTATTGGCCTTTTT
GGTTACACCTATAAAGAAATGCTTATTCGCAGCCCTGCGGTAAAGCATTTTAATGACCGTATGATGTTACGTATCCCAGT
GATTGGCGAGATCCTTAAAAAGGCAGCCGTAGCGCGTTATGCTCGCACACTTTCTACTACCTTTGCTGCGGGTGTTCCCC
TTGGTGATGCGCTCGATTCTGCCGCAGGTGCATCGGGCAATATTATTTATAAAAACGCAATTAAAGAAATTAAAGCAGAG
GTTAACTCGGGTAACCAAATGCACTGGGCAATGCATAACACCAAGGTCTTCCCTGATATGGTGGTGCAAATGGTGTCTAT
CGGTGAAGAATCGGGCTCGCTAGATGGTATGCTGGCAAAGGTTGCCAACATTTATGAGCAAGAAGTCGATGATGCGGTGG
ATGGCCTTTCTAGCCTATTAGAACCATTAATTATGGTGGTCCTCGGCGTACTGATCGGTGGCCTTATCATTGCCATGTAC
TTACCGATATTCCAACTAGGCTCTGTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(77-199)

Type II secretion system (T2SS), protein F (T2SSF)

(281-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JXJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.942

96.829

0.571
  pilC Legionella pneumophila strain ERS1305867

55.037

99.268

0.546
  pilC Acinetobacter baumannii D1279779

51.852

98.78

0.512
  pilC Acinetobacter baylyi ADP1

51.25

97.561

0.5
  pilC Vibrio campbellii strain DS40M4

43.133

100

0.437
  pilC Vibrio cholerae strain A1552

43.284

98.049

0.424
  pilG Neisseria meningitidis 44/76-A

42.965

97.073

0.417
  pilG Neisseria gonorrhoeae MS11

42.965

97.073

0.417

Multiple sequence alignment