Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PNC201_RS04220 Genome accession   NZ_CP022522
Coordinates   1040752..1042428 (-) Length   558 a.a.
NCBI ID   WP_010607343.1    Uniprot ID   A0A2K9JV15
Organism   Pseudoalteromonas sp. NC201     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1035752..1047428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNC201_RS04195 (PNC201_04165) kdsA 1036366..1037220 (+) 855 WP_010370475.1 3-deoxy-8-phosphooctulonate synthase -
  PNC201_RS04200 (PNC201_04170) - 1037306..1038211 (+) 906 WP_088531408.1 transcriptional regulator GcvA -
  PNC201_RS04205 (PNC201_04175) - 1038208..1038846 (+) 639 WP_045989837.1 alpha/beta family hydrolase -
  PNC201_RS04210 (PNC201_04180) - 1038839..1039207 (+) 369 WP_102056260.1 DUF423 domain-containing protein -
  PNC201_RS04215 (PNC201_04185) rlmM 1039221..1040306 (+) 1086 WP_102056261.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  PNC201_RS04220 (PNC201_04190) pilB 1040752..1042428 (-) 1677 WP_010607343.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PNC201_RS04225 (PNC201_04195) coaE 1042538..1043167 (-) 630 WP_102056262.1 dephospho-CoA kinase -
  PNC201_RS04230 (PNC201_04200) pilD 1043177..1044055 (-) 879 WP_102056263.1 A24 family peptidase Machinery gene
  PNC201_RS04235 (PNC201_04205) pilC 1044189..1045421 (-) 1233 WP_102056264.1 type II secretion system F family protein Machinery gene
  PNC201_RS04240 (PNC201_04210) - 1045425..1045898 (-) 474 WP_102056265.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PNC201_RS04245 (PNC201_04215) - 1045885..1046304 (-) 420 WP_102056266.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62014.26 Da        Isoelectric Point: 5.5806

>NTDB_id=240203 PNC201_RS04220 WP_010607343.1 1040752..1042428(-) (pilB) [Pseudoalteromonas sp. NC201]
MEHHSPLLRKFITLGRVTAEQIKSRQSEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCENCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=240203 PNC201_RS04220 WP_010607343.1 1040752..1042428(-) (pilB) [Pseudoalteromonas sp. NC201]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTTACTGCTGAGCAAATAAAGTCTCGCCAGTC
AGAAGCAAACACCACCGCCGAGTTGATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTCAAGGTGCCCTTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCGGCGCTAGTCAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTATGTAGCGGCTTCCGATCCTACTGATTTTGGCGC
ATTTGAGAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTCTTAGTCGCCGACTACAAAAAGCTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGTCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGATAAAGACGATGCGCCAATTATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAGAAAGGCGCGTCGGATCTGCACTTTGAGCCCTACGAAAAAAAATACCGCGTGCGTTTTCGTATCGATGGCCTGC
TGCATGAGATGGCAAGTCCACCAAACACACTGGCTACTCGCCTCGCCGCCCGTATCAAAGTGATGGCGCACTTAGACATA
GCGGAAAAACGTAAACCGCAAGATGGCCGCATTAAATTAAAGATCTCCGAGCGCAAAAGTATCGATTTTCGTGTGAGTAC
ACTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATTGACGTGCTAG
GTTATGAACCAGAGCAAAAGCAGCTTTATCTCGACGCACTAGAGCAGCCTCAGGGCATGATTTTGGTAACCGGCCCAACG
GGTTCCGGTAAAACCGTGTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACGGCGGAAGA
CCCTGTCGAAATAAACCTTGAGGGTATTAACCAAGTACAAATCAATACCAAAGCAGATATGACTTTCGCCAATGCCTTGA
GAGCATTTTTGCGTCAGGATCCGGATGTGGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAGATCTCGATAAAA
GCCGCCCAAACCGGTCACTTAGTATTGAGTACGCTGCACACTAACTCTGCGCCTGAAACACTCACTCGCCTATTAAACAT
GGGCGTGCCAGCATACAACGTAGCAAGCTCGGTGAGTTTGATTATTGCCCAGCGTCTCGCAAGACGCCTTTGTCCTAAGT
GTAAAACACCTGAAACGCTGCCGGAAGAAGCCTTGTTAGGGCAAGGCTTTACTGCCGAGCAAATTAAAGAGATTACCCTT
TTCGCGCCTAAAGGCTGTGAAAACTGTACCGACGGTTACAAAGGCCGTGTGGGTATTTATGAGGTGGTAAAAATCACGCC
AGAGCTTTCGCACCTGATCATGGAAGGGGGCAATTCTCTTGAGATTGCCGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAATCTGGCCTGAAAAAAGCCGCCGCAGGGGTGACATCGCTTACAGAAATCAACCGCGTAACGAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JV15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

53.977

94.624

0.511
  pilB Vibrio campbellii strain DS40M4

53.497

94.803

0.507
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

36.013

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362

Multiple sequence alignment