Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CG419_RS03640 Genome accession   NZ_CP022474
Coordinates   728247..729122 (+) Length   291 a.a.
NCBI ID   WP_089556484.1    Uniprot ID   A0AAC9UP44
Organism   Latilactobacillus curvatus strain MRS6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723247..734122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG419_RS03600 (CG419_03600) - 723354..724181 (-) 828 WP_054644033.1 LysR family transcriptional regulator -
  CG419_RS03605 (CG419_03605) - 724290..725303 (+) 1014 WP_089556482.1 YeiH family protein -
  CG419_RS03610 (CG419_03610) - 725394..725633 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  CG419_RS03615 (CG419_03615) - 725826..726161 (+) 336 WP_089556483.1 hypothetical protein -
  CG419_RS03625 (CG419_03625) - 726342..726719 (-) 378 WP_039099519.1 hypothetical protein -
  CG419_RS03630 (CG419_03630) - 726822..727322 (+) 501 WP_004270893.1 VanZ family protein -
  CG419_RS03635 (CG419_03635) - 727412..728143 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CG419_RS03640 (CG419_03640) comGA 728247..729122 (+) 876 WP_089556484.1 competence type IV pilus ATPase ComGA Machinery gene
  CG419_RS03645 (CG419_03645) comGB 729112..730113 (+) 1002 WP_089556485.1 type II secretion system F family protein Machinery gene
  CG419_RS03650 (CG419_03650) comGC 730110..730418 (+) 309 WP_004270901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CG419_RS03655 (CG419_03655) - 730450..730842 (+) 393 WP_157776705.1 hypothetical protein -
  CG419_RS10460 - 730907..731134 (+) 228 WP_167371741.1 hypothetical protein -
  CG419_RS03660 (CG419_03660) - 731082..731585 (+) 504 WP_167371766.1 competence type IV pilus minor pilin ComGF -
  CG419_RS03665 (CG419_03665) - 731563..731886 (+) 324 WP_039099486.1 hypothetical protein -
  CG419_RS03670 (CG419_03670) - 732002..733021 (+) 1020 WP_338417482.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 31985.83 Da        Isoelectric Point: 5.5900

>NTDB_id=239795 CG419_RS03640 WP_089556484.1 728247..729122(+) (comGA) [Latilactobacillus curvatus strain MRS6]
MTEALTTLLQKCATEKAQDLYFRPLSTGWVIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDPQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVEGQNVAAHYELATAQQLEGAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=239795 CG419_RS03640 WP_089556484.1 728247..729122(+) (comGA) [Latilactobacillus curvatus strain MRS6]
ATGACCGAAGCATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCAAC
GGGTTGGGTAATTATTGAGCGTTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACTGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTCGTTCGTTTCATTTA
TCCCATTGGACAGACTTATCATTGCGACGACCCGCAGATTTTAAGTCGCTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCATTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGAAGGCCAAAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGGGGCTGTTCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.382

93.471

0.405
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

43.019

91.065

0.392
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.852

92.784

0.388
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.852

92.784

0.388
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus gordonii str. Challis substr. CH1

39.925

92.096

0.368
  comYA Streptococcus mutans UA140

39.7

91.753

0.364
  comYA Streptococcus mutans UA159

39.7

91.753

0.364

Multiple sequence alignment