Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CG015_RS03620 Genome accession   NZ_CP022468
Coordinates   744762..746450 (-) Length   562 a.a.
NCBI ID   WP_129532244.1    Uniprot ID   -
Organism   Vibrio anguillarum strain MHK3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739762..751450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG015_RS03590 (CG015_03580) rplS 740480..740833 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  CG015_RS03595 (CG015_03585) yacG 740914..741111 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  CG015_RS03600 (CG015_03590) zapD 741201..741941 (-) 741 WP_013857689.1 cell division protein ZapD -
  CG015_RS03605 (CG015_03595) coaE 741974..742582 (-) 609 WP_047688854.1 dephospho-CoA kinase -
  CG015_RS03610 (CG015_03600) pilD 742573..743454 (-) 882 WP_129532242.1 prepilin peptidase Machinery gene
  CG015_RS03615 (CG015_03605) pilC 743493..744719 (-) 1227 WP_129532243.1 type II secretion system F family protein Machinery gene
  CG015_RS03620 (CG015_03610) pilB 744762..746450 (-) 1689 WP_129532244.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CG015_RS03625 (CG015_03615) pilA 746460..746885 (-) 426 WP_026027561.1 pilin Machinery gene
  CG015_RS03630 (CG015_03620) nadC 747145..748032 (-) 888 WP_017043701.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CG015_RS03635 (CG015_03625) ampD 748157..748708 (+) 552 WP_207204973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CG015_RS03640 (CG015_03630) fldB 748730..749248 (-) 519 WP_013857680.1 flavodoxin FldB -
  CG015_RS03645 (CG015_03635) xerD 749401..750309 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  CG015_RS03650 (CG015_03640) - 750323..751096 (+) 774 WP_013857678.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62222.32 Da        Isoelectric Point: 6.3242

>NTDB_id=239715 CG015_RS03620 WP_129532244.1 744762..746450(-) (pilB) [Vibrio anguillarum strain MHK3]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIANNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLHILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=239715 CG015_RS03620 WP_129532244.1 744762..746450(-) (pilB) [Vibrio anguillarum strain MHK3]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGAAGCTTTGCTTGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCAATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGACTTTCGCTTTGCGACAGGCTTACAAGTCGAACTTGTTTTAGCTGACTACAAAGCACTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACGATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCTAT
CGATATGCGCGTTTCCAGCCTCCCTACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGTGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACACATTTTAAATACTGCTGAAGTGAA
CATTTCTACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGCATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATTGCCGTAAAAGCAGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTAACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.096

100

0.831
  pilB Vibrio campbellii strain DS40M4

74.555

100

0.746
  pilB Vibrio parahaemolyticus RIMD 2210633

73.132

100

0.731
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.19

93.594

0.47
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.932

100

0.377

Multiple sequence alignment